Resazurin assay for assessment of antimicrobial properties of
electrospun nanofiber filtration membranes

J 2019

Resazurin assay for assessment of antimicrobial properties of electrospun nanofiber filtration membranes

TRÁVNÍČKOVÁ, Eva; Přemysl MIKULA; J. OPRSAL; M. BOHACOVA; L. KUBAC et al.

Základní údaje

Originální název

Resazurin assay for assessment of antimicrobial properties of electrospun nanofiber filtration membranes

Autoři

TRÁVNÍČKOVÁ, Eva; Přemysl MIKULA; J. OPRSAL; M. BOHACOVA; L. KUBAC; D. KIMMER; J. SOUKUPOVA a Michal BITTNER

Vydání

AMB EXPRESS, LONDON, SPRINGEROPEN, 2019, 2191-0855

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20801 Environmental biotechnology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.499

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00107010

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Resazurin; Antimicrobial activity; Electrospun nanofiber filtration membranes; Silver nanoparticles; Quaternary ammonium salts

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 3. 2020 12:57, RNDr. Kateřina Nováková, Ph.D.

Anotace

V originále

We developed a simple and fast microplate assay for evaluation of the antimicrobial activity of electrospun nanofiber filtration membranes or similar porous materials for water treatment technologies. Resazurin (alamarBlue (R)) was used as an indicator of the amount of viable experimental microorganisms Gram-negative Escherichia coli, Gram-positive Enterococcus faecalis, and natural wastewater treatment plant effluent bacteria. A bacterial inoculum of concentration 1-3 x 10(5) CFU mL(-1) was pipetted onto the surface of assessed both functionalized and respective control membranes and incubated in 12-well plates for 4 h at 37 degrees C. Kinetics of resazurin metabolization, i.e. its reduction to fluorescent resorufin, was evaluated fluorimetrically (lambda(ex)520/lambda(em)590 nm). A number of viable bacteria on the membranes expressed as CFU mL(-1) was calculated from the kinetic curves by using calibration curves that were constructed for both experimental bacterial species. Antimicrobial activities of the membranes were evaluated by either resazurin assay or modified ISO 20743 plate count assay. Results of both assays showed the significant antimicrobial activity of membranes functionalized with silver nanoparticles for both bacterial species and wastewater treatment plant effluent bacteria as well (log CFU reduction compared to control membrane > 4), while membranes containing specific quaternary ammonium salts were inefficient (log CFU reduction < 1). The suitability of resazurin microplate assay for testing nanofiber filtration membranes and analogous matrices has proven to be a faster and less demanding alternative to the traditionally used approach providing comparable results.

Návaznosti

EF16_013/0001761, projekt VaV

Název: RECETOX RI

FV10323, projekt VaV

Název: Funkční polymery pro aditivaci nanovlákenných filtračních membrán

Investor: Ministerstvo průmyslu a obchodu ČR, Funkční polymery pro aditivaci nanovlákenných filtračních membrán

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

Citovat

TRÁVNÍČKOVÁ, Eva; Přemysl MIKULA; J. OPRSAL; M. BOHACOVA; L. KUBAC; D. KIMMER; J. SOUKUPOVA a Michal BITTNER. Resazurin assay for assessment of antimicrobial properties of electrospun nanofiber filtration membranes. AMB EXPRESS. LONDON: SPRINGEROPEN, 2019, roč. 9, č. 1, s. 1-11. ISSN 2191-0855. Dostupné z: https://doi.org/10.1186/s13568-019-0909-z.

@article{1640456,
   author = {Trávníčková, Eva and Mikula, Přemysl and Oprsal, J. and Bohacova, M. and Kubac, L. and Kimmer, D. and Soukupova, J. and Bittner, Michal},
   article_location = {LONDON},
   article_number = {1},
   doi = {https://doi.org/10.1186/s13568-019-0909-z},
   keywords = {Resazurin; Antimicrobial activity; Electrospun nanofiber filtration membranes; Silver nanoparticles; Quaternary ammonium salts},
   language = {eng},
   issn = {2191-0855},
   journal = {AMB EXPRESS},
   title = {Resazurin assay for assessment of antimicrobial properties of electrospun nanofiber filtration membranes},
   url = {https://link.springer.com/article/10.1186%2Fs13568-019-0909-z},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1640456
AU  - Trávníčková, Eva - Mikula, Přemysl - Oprsal, J. - Bohacova, M. - Kubac, L. - Kimmer, D. - Soukupova, J. - Bittner, Michal
PY  - 2019
TI  - Resazurin assay for assessment of antimicrobial properties of electrospun nanofiber filtration membranes
JF  - AMB EXPRESS
VL  - 9
IS  - 1
SP  - 1-11
EP  - 1-11
PB  - SPRINGEROPEN
SN  - 21910855
KW  - Resazurin
KW  - Antimicrobial activity
KW  - Electrospun nanofiber filtration membranes
KW  - Silver nanoparticles
KW  - Quaternary ammonium salts
UR  - https://link.springer.com/article/10.1186%2Fs13568-019-0909-z
L2  - https://link.springer.com/article/10.1186%2Fs13568-019-0909-z
N2  - We developed a simple and fast microplate assay for evaluation of the antimicrobial activity of electrospun nanofiber filtration membranes or similar porous materials for water treatment technologies. Resazurin (alamarBlue (R)) was used as an indicator of the amount of viable experimental microorganisms Gram-negative Escherichia coli, Gram-positive Enterococcus faecalis, and natural wastewater treatment plant effluent bacteria. A bacterial inoculum of concentration 1-3 x 10(5) CFU mL(-1) was pipetted onto the surface of assessed both functionalized and respective control membranes and incubated in 12-well plates for 4 h at 37 degrees C. Kinetics of resazurin metabolization, i.e. its reduction to fluorescent resorufin, was evaluated fluorimetrically (lambda(ex)520/lambda(em)590 nm). A number of viable bacteria on the membranes expressed as CFU mL(-1) was calculated from the kinetic curves by using calibration curves that were constructed for both experimental bacterial species. Antimicrobial activities of the membranes were evaluated by either resazurin assay or modified ISO 20743 plate count assay. Results of both assays showed the significant antimicrobial activity of membranes functionalized with silver nanoparticles for both bacterial species and wastewater treatment plant effluent bacteria as well (log CFU reduction compared to control membrane > 4), while membranes containing specific quaternary ammonium salts were inefficient (log CFU reduction < 1). The suitability of resazurin microplate assay for testing nanofiber filtration membranes and analogous matrices has proven to be a faster and less demanding alternative to the traditionally used approach providing comparable results.
ER  -

TRÁVNÍČKOVÁ, Eva; Přemysl MIKULA; J. OPRSAL; M. BOHACOVA; L. KUBAC; D. KIMMER; J. SOUKUPOVA a Michal BITTNER. Resazurin assay for assessment of antimicrobial properties of electrospun nanofiber filtration membranes. \textit{AMB EXPRESS}. LONDON: SPRINGEROPEN, 2019, roč.~9, č.~1, s.~1-11. ISSN~2191-0855. Dostupné z: https://doi.org/10.1186/s13568-019-0909-z.

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