Další formáty:
BibTeX
LaTeX
RIS
@inproceedings{1640876,
author = {Vlčnovská, Marcela and Tvrdoňová, Michaela and Vaculovičová, Markéta and Vaculovič, Tomáš},
address = {BRNO},
booktitle = {PROCEEDINGS OF 25TH INTERNATIONAL PHD STUDENTS CONFERENCE (MENDELNET 2018)},
editor = {Cerkal, R.; Belcredi, N.B.; Prokesova, L.},
keywords = {Immunochemistry; dot-blot; gold nanoparticles; LA-ICP-MS},
howpublished = {tištěná verze "print"},
language = {eng},
location = {BRNO},
isbn = {978-80-7509-597-8},
pages = {548-552},
publisher = {MENDEL UNIV BRNO, FAC AGRONOMY},
title = {Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection},
url = {https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf},
year = {2018}
}
TY - JOUR
ID - 1640876
AU - Vlčnovská, Marcela - Tvrdoňová, Michaela - Vaculovičová, Markéta - Vaculovič, Tomáš
PY - 2018
TI - Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection
PB - MENDEL UNIV BRNO, FAC AGRONOMY
CY - BRNO
SN - 9788075095978
KW - Immunochemistry
KW - dot-blot
KW - gold nanoparticles
KW - LA-ICP-MS
UR - https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf
L2 - https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf
N2 - Immunoanalytical techniques are key methods of application in clinical diagnostics, genomics, proteomics and other biochemical and molecular biology disciplines. Most often, they are based on the ability of labeled antibodies to bind specific antigens. It is possible to use a large variety of nanomaterials that are designed, synthesized and adapted to allow highly sensitive detection of advanced immunoassays. Detection can be a highly efficient analytical method of laser ablation followed by inductively coupled plasma mass spectrometry (LA-ICP-MS), which allows the detection of elemental tags suitably conjugated to antibodies. The aim of this work was to conjugate model anti-mouse antibody on a surface of 10nm and 60nm gold nanoparticles and choose the better one for conjugation experimentally by using dot-blot immunobinding assay followed by LA-ICP-MS. It has been experimentally proven that 10nm gold nanoparticles are more suitable for conjugation with antibodies because of lower non-specific sorption on a membrane.
ER -
VLČNOVSKÁ, Marcela, Michaela TVRDOŇOVÁ, Markéta VACULOVIČOVÁ a Tomáš VACULOVIČ. Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection. In Cerkal, R.; Belcredi, N.B.; Prokesova, L. \textit{PROCEEDINGS OF 25TH INTERNATIONAL PHD STUDENTS CONFERENCE (MENDELNET 2018)}. BRNO: MENDEL UNIV BRNO, FAC AGRONOMY, 2018, s.~548-552. ISBN~978-80-7509-597-8.
|
