Utilization of antibody-nanoparticle conjugates as a tool for
immunochemistry with ICP-MS detection

D 2018

Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection

VLČNOVSKÁ, Marcela; Michaela TVRDOŇOVÁ; Markéta VACULOVIČOVÁ a Tomáš VACULOVIČ

Základní údaje

Originální název

Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection

Autoři

VLČNOVSKÁ, Marcela; Michaela TVRDOŇOVÁ; Markéta VACULOVIČOVÁ a Tomáš VACULOVIČ

Vydání

BRNO, PROCEEDINGS OF 25TH INTERNATIONAL PHD STUDENTS CONFERENCE (MENDELNET 2018), od s. 548-552, 5 s. 2018

Nakladatel

MENDEL UNIV BRNO, FAC AGRONOMY

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00113396

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-7509-597-8

UT WoS

000462205300104

Klíčová slova anglicky

Immunochemistry; dot-blot; gold nanoparticles; LA-ICP-MS

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 3. 2020 10:31, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Immunoanalytical techniques are key methods of application in clinical diagnostics, genomics, proteomics and other biochemical and molecular biology disciplines. Most often, they are based on the ability of labeled antibodies to bind specific antigens. It is possible to use a large variety of nanomaterials that are designed, synthesized and adapted to allow highly sensitive detection of advanced immunoassays. Detection can be a highly efficient analytical method of laser ablation followed by inductively coupled plasma mass spectrometry (LA-ICP-MS), which allows the detection of elemental tags suitably conjugated to antibodies. The aim of this work was to conjugate model anti-mouse antibody on a surface of 10nm and 60nm gold nanoparticles and choose the better one for conjugation experimentally by using dot-blot immunobinding assay followed by LA-ICP-MS. It has been experimentally proven that 10nm gold nanoparticles are more suitable for conjugation with antibodies because of lower non-specific sorption on a membrane.

Citovat

VLČNOVSKÁ, Marcela; Michaela TVRDOŇOVÁ; Markéta VACULOVIČOVÁ a Tomáš VACULOVIČ. Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection. In Cerkal, R.; Belcredi, N.B.; Prokesova, L. PROCEEDINGS OF 25TH INTERNATIONAL PHD STUDENTS CONFERENCE (MENDELNET 2018). BRNO: MENDEL UNIV BRNO, FAC AGRONOMY, 2018, s. 548-552. ISBN 978-80-7509-597-8.

@inproceedings{1640876,
   author = {Vlčnovská, Marcela and Tvrdoňová, Michaela and Vaculovičová, Markéta and Vaculovič, Tomáš},
   address = {BRNO},
   booktitle = {PROCEEDINGS OF 25TH INTERNATIONAL PHD STUDENTS CONFERENCE (MENDELNET 2018)},
   editor = {Cerkal, R.; Belcredi, N.B.; Prokesova, L.},
   keywords = {Immunochemistry; dot-blot; gold nanoparticles; LA-ICP-MS},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {BRNO},
   isbn = {978-80-7509-597-8},
   pages = {548-552},
   publisher = {MENDEL UNIV BRNO, FAC AGRONOMY},
   title = {Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection},
   url = {https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf},
   year = {2018}
}

TY  - CONF
ID  - 1640876
AU  - Vlčnovská, Marcela - Tvrdoňová, Michaela - Vaculovičová, Markéta - Vaculovič, Tomáš
PY  - 2018
TI  - Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection
PB  - MENDEL UNIV BRNO, FAC AGRONOMY
CY  - BRNO
SN  - 9788075095978
KW  - Immunochemistry
KW  - dot-blot
KW  - gold nanoparticles
KW  - LA-ICP-MS
UR  - https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf
L2  - https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf
N2  - Immunoanalytical techniques are key methods of application in clinical diagnostics, genomics, proteomics and other biochemical and molecular biology disciplines. Most often, they are based on the ability of labeled antibodies to bind specific antigens. It is possible to use a large variety of nanomaterials that are designed, synthesized and adapted to allow highly sensitive detection of advanced immunoassays. Detection can be a highly efficient analytical method of laser ablation followed by inductively coupled plasma mass spectrometry (LA-ICP-MS), which allows the detection of elemental tags suitably conjugated to antibodies. The aim of this work was to conjugate model anti-mouse antibody on a surface of 10nm and 60nm gold nanoparticles and choose the better one for conjugation experimentally by using dot-blot immunobinding assay followed by LA-ICP-MS. It has been experimentally proven that 10nm gold nanoparticles are more suitable for conjugation with antibodies because of lower non-specific sorption on a membrane.
ER  -

VLČNOVSKÁ, Marcela; Michaela TVRDOŇOVÁ; Markéta VACULOVIČOVÁ a Tomáš VACULOVIČ. Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection. In Cerkal, R.; Belcredi, N.B.; Prokesova, L. \textit{PROCEEDINGS OF 25TH INTERNATIONAL PHD STUDENTS CONFERENCE (MENDELNET 2018)}. BRNO: MENDEL UNIV BRNO, FAC AGRONOMY, 2018, s.~548-552. ISBN~978-80-7509-597-8.

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