MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic
Stem Cells

J 2019

MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells

ŠTEFKOVÁ, Kateřina; Markéta HANÁČKOVÁ; Jan KUČERA; Katarzyna Anna RADASZKIEWICZ; Barbora AMBRŮZOVÁ et al.

Základní údaje

Originální název

MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells

Autoři

ŠTEFKOVÁ, Kateřina; Markéta HANÁČKOVÁ; Jan KUČERA; Katarzyna Anna RADASZKIEWICZ; Barbora AMBRŮZOVÁ; Lukáš KUBALA a Jiří PACHERNÍK

Vydání

Stem Cells International, London, HINDAWI LTD, 2019, 1687-966X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.869

Kód RIV

RIV/00216224:14310/19:00108234

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1155/2019/5128135

UT WoS

000472008000001

EID Scopus

2-s2.0-85071471748

Klíčová slova anglicky

C/EBP

Štítky

rivok

Změněno: 2. 4. 2020 10:21, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The activation of p38alpha kinase mediates cell response to various extracellular factors including many interleukins and growth factors important for haematopoiesis. The role of p38alpha kinase was previously analysed in particular haematopoietic cells. In this study and for the first time, the role of p38alpha kinase in haematopoiesis was studied using a model of continuous haematopoietic development in pluripotent embryonic stem cells in vitro. The expression of transcripts associated with haematopoiesis and the potential for the formation of specific haematopoietic cell colonies were compared between wild-type and mutant p38alpha gene-depleted cells. The absence of p38alpha kinase led to the inhibition of hemangioblast formation during the first step of haematopoiesis. Later, during differentiation, due to the lack of p38alpha kinase, erythrocyte maturation was impaired. Mutant p38-/- cells also exhibited decreased potential with respect to the expansion of granulocyte colony-forming units. This effect was reversed in the absence of erythropoietin as shown by colony-forming unit assay in media for colony-forming unit granulocytes/macrophages. p38alpha kinase thus plays an important role in the differentiation of common myeloid precursor cells into granulocyte lineages.

Návaznosti

GA17-05466S, projekt VaV

Název: Role kanonické signální dráhy Wnt v neurogenezi. (Akronym: WntNeuro)

Investor: Grantová agentura ČR, Role kanonické signální dráhy Wnt v neurogenezi.

MUNI/A/1145/2017, interní kód MU

Název: Podpora výzkumné činnosti studentů v oblasti fyziologie, vývojové biologie a imunologie živočichů v roce 2018 (Akronym: OFIŽ 2018)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů v oblasti fyziologie, vývojové biologie a imunologie živočichů v roce 2018, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

ŠTEFKOVÁ, Kateřina; Markéta HANÁČKOVÁ; Jan KUČERA; Katarzyna Anna RADASZKIEWICZ; Barbora AMBRŮZOVÁ; Lukáš KUBALA a Jiří PACHERNÍK. MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells. Stem Cells International. London: HINDAWI LTD, 2019, roč. 2019, č. 2019, s. 1-16. ISSN 1687-966X. Dostupné z: https://doi.org/10.1155/2019/5128135.

@article{1642040,
   author = {Štefková, Kateřina and Hanáčková, Markéta and Kučera, Jan and Radaszkiewicz, Katarzyna Anna and Ambrůzová, Barbora and Kubala, Lukáš and Pacherník, Jiří},
   article_location = {London},
   article_number = {2019},
   doi = {https://doi.org/10.1155/2019/5128135},
   keywords = {C/EBP},
   language = {eng},
   issn = {1687-966X},
   journal = {Stem Cells International},
   title = {MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells},
   url = {https://www.hindawi.com/journals/sci/2019/5128135/},
   volume = {2019},
   year = {2019}
}

TY  - JOUR
ID  - 1642040
AU  - Štefková, Kateřina - Hanáčková, Markéta - Kučera, Jan - Radaszkiewicz, Katarzyna Anna - Ambrůzová, Barbora - Kubala, Lukáš - Pacherník, Jiří
PY  - 2019
TI  - MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells
JF  - Stem Cells International
VL  - 2019
IS  - 2019
SP  - 1-16
EP  - 1-16
PB  - HINDAWI LTD
SN  - 1687966X
KW  - C/EBP
UR  - https://www.hindawi.com/journals/sci/2019/5128135/
L2  - https://www.hindawi.com/journals/sci/2019/5128135/
N2  - The activation of p38alpha kinase mediates cell response to various extracellular factors including many interleukins and growth factors important for haematopoiesis. The role of p38alpha kinase was previously analysed in particular haematopoietic cells. In this study and for the first time, the role of p38alpha kinase in haematopoiesis was studied using a model of continuous haematopoietic development in pluripotent embryonic stem cells in vitro. The expression of transcripts associated with haematopoiesis and the potential for the formation of specific haematopoietic cell colonies were compared between wild-type and mutant p38alpha gene-depleted cells. The absence of p38alpha kinase led to the inhibition of hemangioblast formation during the first step of haematopoiesis. Later, during differentiation, due to the lack of p38alpha kinase, erythrocyte maturation was impaired. Mutant p38-/- cells also exhibited decreased potential with respect to the expansion of granulocyte colony-forming units. This effect was reversed in the absence of erythropoietin as shown by colony-forming unit assay in media for colony-forming unit granulocytes/macrophages. p38alpha kinase thus plays an important role in the differentiation of common myeloid precursor cells into granulocyte lineages.
ER  -

ŠTEFKOVÁ, Kateřina; Markéta HANÁČKOVÁ; Jan KUČERA; Katarzyna Anna RADASZKIEWICZ; Barbora AMBRŮZOVÁ; Lukáš KUBALA a Jiří PACHERNÍK. MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells. \textit{Stem Cells International}. London: HINDAWI LTD, 2019, roč.~2019, č.~2019, s.~1-16. ISSN~1687-966X. Dostupné z: https://doi.org/10.1155/2019/5128135.

Přehled o publikaci