A Distinct, Sequence-Induced Conformation Is Required for
Recognition of the Constitutive Decay Element RNA by Roquin

J 2015

A Distinct, Sequence-Induced Conformation Is Required for Recognition of the Constitutive Decay Element RNA by Roquin

CODUTTI, L., K. LEPPEK, Jan ZÁLEŠÁK, V. WINDEISEN, P. MASIEWICZ et. al.

Základní údaje

Originální název

A Distinct, Sequence-Induced Conformation Is Required for Recognition of the Constitutive Decay Element RNA by Roquin

Autoři

CODUTTI, L., K. LEPPEK, Jan ZÁLEŠÁK, V. WINDEISEN, P. MASIEWICZ, G. STOECKLIN a T. CARLOMAGNO

Vydání

Structure, CAMBRIDGE, CELL PRESS, 2015, 0969-2126

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.237

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.str.2015.06.001

UT WoS

000361112300009

Změněno: 21. 4. 2020 14:37, Mgr. Marie Šípková, DiS.

Anotace

V originále

The constitutive decay element (CDE) of tumor necrosis factor alpha (TNF-alpha) mRNA (Tnf) represents the prototype of a class of RNA motifs that mediate rapid degradation of mRNAs encoding regulators of the immune response and development. CDE-type RNAs are hairpin structures featuring a tri-nucleotide loop. The protein Roquin recognizes CDE-type stem loops and recruits the Ccr4-Caf1-Not deadenylase complex to the mRNA, thereby inducing its decay. Stem recognition does not involve nucleotide bases; however, there is a strong stem sequence requirement for functional CDEs. Here, we present the solution structures of the natural Tnf CDE and of a CDE mutant with impaired Roquin binding. We find that the two CDEs adopt unique and distinct structures in both the loop and the stem, which explains the ability of Roquin to recognize stem loops in a sequence-specific manner. Our findings result in a relaxed consensus motif for prediction of new CDE stem loops.

Citovat

CODUTTI, L., K. LEPPEK, Jan ZÁLEŠÁK, V. WINDEISEN, P. MASIEWICZ, G. STOECKLIN a T. CARLOMAGNO. A Distinct, Sequence-Induced Conformation Is Required for Recognition of the Constitutive Decay Element RNA by Roquin. Structure. CAMBRIDGE: CELL PRESS, 2015, roč. 23, č. 8, s. 1437-1447. ISSN 0969-2126. Dostupné z: https://dx.doi.org/10.1016/j.str.2015.06.001.

@article{1647977,
   author = {Codutti, L. and Leppek, K. and Zálešák, Jan and Windeisen, V. and Masiewicz, P. and Stoecklin, G. and Carlomagno, T.},
   article_location = {CAMBRIDGE},
   article_number = {8},
   doi = {http://dx.doi.org/10.1016/j.str.2015.06.001},
   language = {eng},
   issn = {0969-2126},
   journal = {Structure},
   note = {Žádný z autorů nemá afiliaci pro MU.},
   title = {A Distinct, Sequence-Induced Conformation Is Required for Recognition of the Constitutive Decay Element RNA by Roquin},
   volume = {23},
   year = {2015}
}

TY  - JOUR
ID  - 1647977
AU  - Codutti, L. - Leppek, K. - Zálešák, Jan - Windeisen, V. - Masiewicz, P. - Stoecklin, G. - Carlomagno, T.
PY  - 2015
TI  - A Distinct, Sequence-Induced Conformation Is Required for Recognition of the Constitutive Decay Element RNA by Roquin
JF  - Structure
VL  - 23
IS  - 8
SP  - 1437-1447
EP  - 1437-1447
PB  - CELL PRESS
SN  - 09692126
N1  - Žádný z autorů nemá afiliaci pro MU.
N2  - The constitutive decay element (CDE) of tumor necrosis factor alpha (TNF-alpha) mRNA (Tnf) represents the prototype of a class of RNA motifs that mediate rapid degradation of mRNAs encoding regulators of the immune response and development. CDE-type RNAs are hairpin structures featuring a tri-nucleotide loop. The protein Roquin recognizes CDE-type stem loops and recruits the Ccr4-Caf1-Not deadenylase complex to the mRNA, thereby inducing its decay. Stem recognition does not involve nucleotide bases; however, there is a strong stem sequence requirement for functional CDEs. Here, we present the solution structures of the natural Tnf CDE and of a CDE mutant with impaired Roquin binding. We find that the two CDEs adopt unique and distinct structures in both the loop and the stem, which explains the ability of Roquin to recognize stem loops in a sequence-specific manner. Our findings result in a relaxed consensus motif for prediction of new CDE stem loops.
ER  -

CODUTTI, L., K. LEPPEK, Jan ZÁLEŠÁK, V. WINDEISEN, P. MASIEWICZ, G. STOECKLIN a T. CARLOMAGNO. A Distinct, Sequence-Induced Conformation Is Required for Recognition of the Constitutive Decay Element RNA by Roquin. \textit{Structure}. CAMBRIDGE: CELL PRESS, 2015, roč.~23, č.~8, s.~1437-1447. ISSN~0969-2126. Dostupné z: https://dx.doi.org/10.1016/j.str.2015.06.001.

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