Plasma-Coated Polycaprolactone Nanofibers with Covalently
Bonded Platelet-Rich Plasma Enhance Adhesion and Growth of
Human Fibroblasts

J 2019

Plasma-Coated Polycaprolactone Nanofibers with Covalently Bonded Platelet-Rich Plasma Enhance Adhesion and Growth of Human Fibroblasts

MIROSHNICHENKO, S.; V. TIMOFEEVA; E. PERMYAKOVA; S. ERSHOV; P. KIRYUKHANTSEV-KORNEEV et al.

Základní údaje

Originální název

Plasma-Coated Polycaprolactone Nanofibers with Covalently Bonded Platelet-Rich Plasma Enhance Adhesion and Growth of Human Fibroblasts

Autoři

MIROSHNICHENKO, S.; V. TIMOFEEVA; E. PERMYAKOVA; S. ERSHOV; P. KIRYUKHANTSEV-KORNEEV; E. DVORAKOVA; D.V. SHTANSKY; Lenka ZAJÍČKOVÁ; A. SOLOVIEVA a A. MANAKHOV

Vydání

Nanomaterials, Basel, MDPI, 2019, 2079-4991

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

21001 Nano-materials

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.324

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/19:00113753

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

polycaprolactone; nanofibers; COOH plasma; cell adhesion and spreading; cell viability; freeze-thawed platelet-rich plasma immobilization

Změněno: 23. 4. 2020 15:42, Mgr. Michal Petr

Anotace

V originále

Biodegradable nanofibers are extensively employed in different areas of biology and medicine, particularly in tissue engineering. The electrospun polycaprolactone (PCL) nanofibers are attracting growing interest due to their good mechanical properties and a low-cost structure similar to the extracellular matrix. However, the unmodified PCL nanofibers exhibit an inert surface, hindering cell adhesion and negatively affecting their further fate. The employment of PCL nanofibrous scaffolds for wound healing requires a certain modification of the PCL surface. In this work, the morphology of PCL nanofibers is optimized by the careful tuning of electrospinning parameters. It is shown that the modification of the PCL nanofibers with the COOH plasma polymers and the subsequent binding of NH2 groups of protein molecules is a rather simple and technologically accessible procedure allowing the adhesion, early spreading, and growth of human fibroblasts to be boosted. The behavior of fibroblasts on the modified PCL surface was found to be very different when compared to the previously studied cultivation of mesenchymal stem cells on the PCL nanofibrous meshes. It is demonstrated by X-ray photoelectron spectroscopy (XPS) that the freeze-thawed platelet-rich plasma (PRP) immobilization can be performed via covalent and non-covalent bonding and that it does not affect biological activity. The covalently bound components of PRP considerably reduce the fibroblast apoptosis and increase the cell proliferation in comparison to the unmodified PCL nanofibers or the PCL nanofibers with non-covalent bonding of PRP. The reported research findings reveal the potential of PCL matrices for application in tissue engineering, while the plasma modification with COOH groups and their subsequent covalent binding with proteins expand this potential even further. The use of such matrices with covalently immobilized PRP for wound healing leads to prolonged biological activity of the immobilized molecules and protects these biomolecules from the aggressive media of the wound.

Citovat

MIROSHNICHENKO, S.; V. TIMOFEEVA; E. PERMYAKOVA; S. ERSHOV; P. KIRYUKHANTSEV-KORNEEV; E. DVORAKOVA; D.V. SHTANSKY; Lenka ZAJÍČKOVÁ; A. SOLOVIEVA a A. MANAKHOV. Plasma-Coated Polycaprolactone Nanofibers with Covalently Bonded Platelet-Rich Plasma Enhance Adhesion and Growth of Human Fibroblasts. Nanomaterials. Basel: MDPI, 2019, roč. 9, č. 4, s. 1-19. ISSN 2079-4991. Dostupné z: https://doi.org/10.3390/nano9040637.

@article{1648900,
   author = {Miroshnichenko, S. and Timofeeva, V. and Permyakova, E. and Ershov, S. and KiryukhantsevandKorneev, P. and Dvorakova, E. and Shtansky, D.V. and Zajíčková, Lenka and Solovieva, A. and Manakhov, A.},
   article_location = {Basel},
   article_number = {4},
   doi = {https://doi.org/10.3390/nano9040637},
   keywords = {polycaprolactone; nanofibers; COOH plasma; cell adhesion and spreading; cell viability; freeze-thawed platelet-rich plasma immobilization},
   language = {eng},
   issn = {2079-4991},
   journal = {Nanomaterials},
   title = {Plasma-Coated Polycaprolactone Nanofibers with Covalently Bonded Platelet-Rich Plasma Enhance Adhesion and Growth of Human Fibroblasts},
   url = {http://dx.doi.org/10.3390/nano9040637},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1648900
AU  - Miroshnichenko, S. - Timofeeva, V. - Permyakova, E. - Ershov, S. - Kiryukhantsev-Korneev, P. - Dvorakova, E. - Shtansky, D.V. - Zajíčková, Lenka - Solovieva, A. - Manakhov, A.
PY  - 2019
TI  - Plasma-Coated Polycaprolactone Nanofibers with Covalently Bonded Platelet-Rich Plasma Enhance Adhesion and Growth of Human Fibroblasts
JF  - Nanomaterials
VL  - 9
IS  - 4
SP  - 1-19
EP  - 1-19
PB  - MDPI
SN  - 20794991
KW  - polycaprolactone
KW  - nanofibers
KW  - COOH plasma
KW  - cell adhesion and spreading
KW  - cell viability
KW  - freeze-thawed platelet-rich plasma immobilization
UR  - http://dx.doi.org/10.3390/nano9040637
L2  - http://dx.doi.org/10.3390/nano9040637
N2  - Biodegradable nanofibers are extensively employed in different areas of biology and medicine, particularly in tissue engineering. The electrospun polycaprolactone (PCL) nanofibers are attracting growing interest due to their good mechanical properties and a low-cost structure similar to the extracellular matrix. However, the unmodified PCL nanofibers exhibit an inert surface, hindering cell adhesion and negatively affecting their further fate. The employment of PCL nanofibrous scaffolds for wound healing requires a certain modification of the PCL surface. In this work, the morphology of PCL nanofibers is optimized by the careful tuning of electrospinning parameters. It is shown that the modification of the PCL nanofibers with the COOH plasma polymers and the subsequent binding of NH2 groups of protein molecules is a rather simple and technologically accessible procedure allowing the adhesion, early spreading, and growth of human fibroblasts to be boosted. The behavior of fibroblasts on the modified PCL surface was found to be very different when compared to the previously studied cultivation of mesenchymal stem cells on the PCL nanofibrous meshes. It is demonstrated by X-ray photoelectron spectroscopy (XPS) that the freeze-thawed platelet-rich plasma (PRP) immobilization can be performed via covalent and non-covalent bonding and that it does not affect biological activity. The covalently bound components of PRP considerably reduce the fibroblast apoptosis and increase the cell proliferation in comparison to the unmodified PCL nanofibers or the PCL nanofibers with non-covalent bonding of PRP. The reported research findings reveal the potential of PCL matrices for application in tissue engineering, while the plasma modification with COOH groups and their subsequent covalent binding with proteins expand this potential even further. The use of such matrices with covalently immobilized PRP for wound healing leads to prolonged biological activity of the immobilized molecules and protects these biomolecules from the aggressive media of the wound.
ER  -

MIROSHNICHENKO, S.; V. TIMOFEEVA; E. PERMYAKOVA; S. ERSHOV; P. KIRYUKHANTSEV-KORNEEV; E. DVORAKOVA; D.V. SHTANSKY; Lenka ZAJÍČKOVÁ; A. SOLOVIEVA a A. MANAKHOV. Plasma-Coated Polycaprolactone Nanofibers with Covalently Bonded Platelet-Rich Plasma Enhance Adhesion and Growth of Human Fibroblasts. \textit{Nanomaterials}. Basel: MDPI, 2019, roč.~9, č.~4, s.~1-19. ISSN~2079-4991. Dostupné z: https://doi.org/10.3390/nano9040637.

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