PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE
LABEL IN IMMUNOASSAYS

D 2018

PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS

FARKA, Zdeněk; A. HLAVACEK; M.J. MICKERT; Petr SKLÁDAL; Hans-Heiner GORRIS et al.

Základní údaje

Originální název

PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS

Autoři

FARKA, Zdeněk; A. HLAVACEK; M.J. MICKERT; Petr SKLÁDAL a Hans-Heiner GORRIS

Vydání

SLEZSKA, 9TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2017), od s. 455-460, 6 s. 2018

Nakladatel

TANGER LTD

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/18:00113779

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-87294-81-9

UT WoS

000452823300074

EID Scopus

2-s2.0-85051857031

Klíčová slova anglicky

Photon-upconversion nanoparticle; immunoassay; bioconjugation; antibody; diclofenac

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 4. 2020 12:22, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg.mL(-1).

Návaznosti

LD15023, projekt VaV

Název: Příprava diskrétních biokonjugátů photon-upconversion nanočástic

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Příprava diskrétních biokonjugátů photon-upconversion nanočástic

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

FARKA, Zdeněk; A. HLAVACEK; M.J. MICKERT; Petr SKLÁDAL a Hans-Heiner GORRIS. PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS. Online. In 9TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2017). SLEZSKA: TANGER LTD, 2018, s. 455-460. ISBN 978-80-87294-81-9.

@inproceedings{1649198,
   author = {Farka, Zdeněk and Hlavacek, A. and Mickert, M.J. and Skládal, Petr and Gorris, HansandHeiner},
   address = {SLEZSKA},
   booktitle = {9TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2017)},
   keywords = {Photon-upconversion nanoparticle; immunoassay; bioconjugation; antibody; diclofenac},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {SLEZSKA},
   isbn = {978-80-87294-81-9},
   pages = {455-460},
   publisher = {TANGER LTD},
   title = {PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS},
   year = {2018}
}

TY  - CONF
ID  - 1649198
AU  - Farka, Zdeněk - Hlavacek, A. - Mickert, M.J. - Skládal, Petr - Gorris, Hans-Heiner
PY  - 2018
TI  - PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS
PB  - TANGER LTD
CY  - SLEZSKA
SN  - 9788087294819
KW  - Photon-upconversion nanoparticle
KW  - immunoassay
KW  - bioconjugation
KW  - antibody
KW  - diclofenac
N2  - Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg.mL(-1).
ER  -

FARKA, Zdeněk; A. HLAVACEK; M.J. MICKERT; Petr SKLÁDAL a Hans-Heiner GORRIS. PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS. Online. In \textit{9TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH \&{}amp; APPLICATION (NANOCON 2017)}. SLEZSKA: TANGER LTD, 2018, s.~455-460. ISBN~978-80-87294-81-9.

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