Další formáty:
BibTeX
LaTeX
RIS
@inproceedings{1649198,
author = {Farka, Zdeněk and Hlavacek, A. and Mickert, M.J. and Skládal, Petr and Gorris, HansandHeiner},
address = {SLEZSKA},
booktitle = {9TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2017)},
keywords = {Photon-upconversion nanoparticle; immunoassay; bioconjugation; antibody; diclofenac},
howpublished = {elektronická verze "online"},
language = {eng},
location = {SLEZSKA},
isbn = {978-80-87294-81-9},
pages = {455-460},
publisher = {TANGER LTD},
title = {PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS},
year = {2018}
}
TY - JOUR
ID - 1649198
AU - Farka, Zdeněk - Hlavacek, A. - Mickert, M.J. - Skládal, Petr - Gorris, Hans-Heiner
PY - 2018
TI - PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS
PB - TANGER LTD
CY - SLEZSKA
SN - 9788087294819
KW - Photon-upconversion nanoparticle
KW - immunoassay
KW - bioconjugation
KW - antibody
KW - diclofenac
N2 - Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg.mL(-1).
ER -
FARKA, Zdeněk, A. HLAVACEK, M.J. MICKERT, Petr SKLÁDAL a Hans-Heiner GORRIS. PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS. Online. In \textit{9TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH \&{}amp; APPLICATION (NANOCON 2017)}. SLEZSKA: TANGER LTD, 2018, s.~455-460. ISBN~978-80-87294-81-9.
|
