Significant Down-Regulation of

J 2017

Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM

BUDNA, J.; P. CELICHOWSKI; A. BRYJA; M. DYSZKIEWICZ-KONWINSKA; Michal JEŠETA et al.

Základní údaje

Originální název

Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM

Autoři

BUDNA, J.; P. CELICHOWSKI; A. BRYJA; M. DYSZKIEWICZ-KONWINSKA; Michal JEŠETA; D. BUKOWSKA; P. ANTOSIK; K. P. BRUSSOW; M. BRUSKA; M. NOWICKI; M. ZABEL a B. KEMPISTY

Vydání

International Journal of Molecular Sciences, Basel, Switzerland, MDPI AG, 2017, 1661-6596

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.687

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/17:00113801

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

pig; oocytes; gamete biology; molecular biology; in vitro maturation (IVM)

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 5. 2020 11:26, Mgr. Tereza Miškechová

Anotace

V originále

Proper maturation of the mammalian oocyte is a compound processes determining successful monospermic fertilization, however the number of fully mature porcine oocytes is still unsatisfactory. Since oocytes' maturation and fertilization involve cellular adhesion and membranous contact, the aim was to investigate cell adhesion ontology group in porcine oocytes. The oocytes were collected from ovaries of 45 pubertal crossbred Landrace gilts and subjected to two BCB tests. After the first test, only granulosa cell-free BCB+ oocytes were directly exposed to microarray assays and RT-qPCR ("before IVM" group), or first in vitro matured and then if classified as BCB+ passed to molecular analyses ("after IVM" group). As a result, we have discovered substantial down-regulation of genes involved in adhesion processes, such as: organization of actin cytoskeleton, migration, proliferation, differentiation, apoptosis, survival or angiogenesis in porcine oocytes after IVM, compared to oocytes analyzed before IVM. In conclusion, we found that biological adhesion may be recognized as the process involved in porcine oocytes' successful IVM. Down-regulation of genes included in this ontology group in immature oocytes after IVM points to their unique function in oocyte's achievement of fully mature stages. Thus, results indicated new molecular markers involved in porcine oocyte IVM, displaying essential roles in biological adhesion processes.

Citovat

BUDNA, J.; P. CELICHOWSKI; A. BRYJA; M. DYSZKIEWICZ-KONWINSKA; Michal JEŠETA; D. BUKOWSKA; P. ANTOSIK; K. P. BRUSSOW; M. BRUSKA; M. NOWICKI; M. ZABEL a B. KEMPISTY. Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM. International Journal of Molecular Sciences. Basel, Switzerland: MDPI AG, 2017, roč. 18, č. 12, s. 1-19. ISSN 1661-6596. Dostupné z: https://doi.org/10.3390/ijms18122685.

@article{1649937,
   author = {Budna, J. and Celichowski, P. and Bryja, A. and DyszkiewiczandKonwinska, M. and Ješeta, Michal and Bukowska, D. and Antosik, P. and Brussow, K. P. and Bruska, M. and Nowicki, M. and Zabel, M. and Kempisty, B.},
   article_location = {Basel, Switzerland},
   article_number = {12},
   doi = {https://doi.org/10.3390/ijms18122685},
   keywords = {pig; oocytes; gamete biology; molecular biology; in vitro maturation (IVM)},
   language = {eng},
   issn = {1661-6596},
   journal = {International Journal of Molecular Sciences},
   title = {Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM},
   url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751287/},
   volume = {18},
   year = {2017}
}

TY  - JOUR
ID  - 1649937
AU  - Budna, J. - Celichowski, P. - Bryja, A. - Dyszkiewicz-Konwinska, M. - Ješeta, Michal - Bukowska, D. - Antosik, P. - Brussow, K. P. - Bruska, M. - Nowicki, M. - Zabel, M. - Kempisty, B.
PY  - 2017
TI  - Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM
JF  - International Journal of Molecular Sciences
VL  - 18
IS  - 12
SP  - 1-19
EP  - 1-19
PB  - MDPI AG
SN  - 16616596
KW  - pig
KW  - oocytes
KW  - gamete biology
KW  - molecular biology
KW  - in vitro maturation (IVM)
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751287/
L2  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751287/
N2  - Proper maturation of the mammalian oocyte is a compound processes determining successful monospermic fertilization, however the number of fully mature porcine oocytes is still unsatisfactory. Since oocytes' maturation and fertilization involve cellular adhesion and membranous contact, the aim was to investigate cell adhesion ontology group in porcine oocytes. The oocytes were collected from ovaries of 45 pubertal crossbred Landrace gilts and subjected to two BCB tests. After the first test, only granulosa cell-free BCB+ oocytes were directly exposed to microarray assays and RT-qPCR ("before IVM" group), or first in vitro matured and then if classified as BCB+ passed to molecular analyses ("after IVM" group). As a result, we have discovered substantial down-regulation of genes involved in adhesion processes, such as: organization of actin cytoskeleton, migration, proliferation, differentiation, apoptosis, survival or angiogenesis in porcine oocytes after IVM, compared to oocytes analyzed before IVM. In conclusion, we found that biological adhesion may be recognized as the process involved in porcine oocytes' successful IVM. Down-regulation of genes included in this ontology group in immature oocytes after IVM points to their unique function in oocyte's achievement of fully mature stages. Thus, results indicated new molecular markers involved in porcine oocyte IVM, displaying essential roles in biological adhesion processes.
ER  -

BUDNA, J.; P. CELICHOWSKI; A. BRYJA; M. DYSZKIEWICZ-KONWINSKA; Michal JEŠETA; D. BUKOWSKA; P. ANTOSIK; K. P. BRUSSOW; M. BRUSKA; M. NOWICKI; M. ZABEL a B. KEMPISTY. Significant Down-Regulation of ''Biological Adhesion'' Genes in Porcine Oocytes after IVM. \textit{International Journal of Molecular Sciences}. Basel, Switzerland: MDPI AG, 2017, roč.~18, č.~12, s.~1-19. ISSN~1661-6596. Dostupné z: https://doi.org/10.3390/ijms18122685.

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