Differential cellular localization of Epstein-Barr virus and
human cytomegalovirus in the colonic mucosa of patients with
active or quiescent inflammatory bowel disease

J 2016

Differential cellular localization of Epstein-Barr virus and human cytomegalovirus in the colonic mucosa of patients with active or quiescent inflammatory bowel disease

CICCOCIOPPO, R.; F. RACCA; L. SCUDELLER; A. PIRALLA; P. FORMAGNANA et al.

Základní údaje

Originální název

Differential cellular localization of Epstein-Barr virus and human cytomegalovirus in the colonic mucosa of patients with active or quiescent inflammatory bowel disease

Autoři

CICCOCIOPPO, R.; F. RACCA; L. SCUDELLER; A. PIRALLA; P. FORMAGNANA; L. POZZI; E. BETTI; A. VANOLI; R. RIBONI; Peter KRUŽLIAK; F. BALDANTI a G. R. CORAZZA

Vydání

Immunologic Research, Totowa, Humana Press, 2016, 0257-277X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.905

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/16:00120608

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Enterocytes; Inflammatory bowel disease; Mucosa; Quantitative real-time PCR; Viral infection

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 5. 2022 14:51, Mgr. Tereza Miškechová

Anotace

V originále

The role of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) in the exacerbation of inflammatory bowel disease (IBD) is still uncertain. We prospectively investigated the presence of EBV and HCMV infection in both epithelial and immune cells of colonic mucosa of IBD patients, both refractory and responders to standard therapies, in comparison with patients suffering from irritable bowel syndrome who were considered as controls, by using quantitative real-time polymerase chain reaction, immunohistochemistry and in situ hybridization, in an attempt to assess viral localization, DNA load, life cycle phase and possible correlation with disease activity indexes. We obtained clear evidence of the presence of high DNA loads of both viruses in either enterocytes or immune cells of refractory IBD patients, whereas we observed low levels in the responder group and an absence of detectable copies in all cell populations of controls. Remarkably, the values of EBV and HCMV DNA in inflamed mucosa were invariably higher than in non-inflamed areas in both IBD groups, and the EBV DNA loads in the cell populations of diseased mucosa of refractory IBD patients positively correlated with the severity of mucosal damage and clinical indexes of activity. Moreover, EBV infection resulted the most prevalent either alone or in combination with HCMV, while immunohistochemistry and in situ hybridization did not allow us to distinguish between the different phases of viral life cycle. Finally, as regards treatment, these novel findings could pave the way for the use of new antiviral molecules in the treatment of this condition.

Citovat

CICCOCIOPPO, R.; F. RACCA; L. SCUDELLER; A. PIRALLA; P. FORMAGNANA; L. POZZI; E. BETTI; A. VANOLI; R. RIBONI; Peter KRUŽLIAK; F. BALDANTI a G. R. CORAZZA. Differential cellular localization of Epstein-Barr virus and human cytomegalovirus in the colonic mucosa of patients with active or quiescent inflammatory bowel disease. Immunologic Research. Totowa: Humana Press, 2016, roč. 64, č. 1, s. 191-203. ISSN 0257-277X. Dostupné z: https://doi.org/10.1007/s12026-015-8737-y.

@article{1650058,
   author = {Ciccocioppo, R. and Racca, F. and Scudeller, L. and Piralla, A. and Formagnana, P. and Pozzi, L. and Betti, E. and Vanoli, A. and Riboni, R. and Kružliak, Peter and Baldanti, F. and Corazza, G. R.},
   article_location = {Totowa},
   article_number = {1},
   doi = {https://doi.org/10.1007/s12026-015-8737-y},
   keywords = {Enterocytes; Inflammatory bowel disease; Mucosa; Quantitative real-time PCR; Viral infection},
   language = {eng},
   issn = {0257-277X},
   journal = {Immunologic Research},
   title = {Differential cellular localization of Epstein-Barr virus and human cytomegalovirus in the colonic mucosa of patients with active or quiescent inflammatory bowel disease},
   url = {https://link.springer.com/article/10.1007%2Fs12026-015-8737-y},
   volume = {64},
   year = {2016}
}

TY  - JOUR
ID  - 1650058
AU  - Ciccocioppo, R. - Racca, F. - Scudeller, L. - Piralla, A. - Formagnana, P. - Pozzi, L. - Betti, E. - Vanoli, A. - Riboni, R. - Kružliak, Peter - Baldanti, F. - Corazza, G. R.
PY  - 2016
TI  - Differential cellular localization of Epstein-Barr virus and human cytomegalovirus in the colonic mucosa of patients with active or quiescent inflammatory bowel disease
JF  - Immunologic Research
VL  - 64
IS  - 1
SP  - 191-203
EP  - 191-203
PB  - Humana Press
SN  - 0257277X
KW  - Enterocytes
KW  - Inflammatory bowel disease
KW  - Mucosa
KW  - Quantitative real-time PCR
KW  - Viral infection
UR  - https://link.springer.com/article/10.1007%2Fs12026-015-8737-y
L2  - https://link.springer.com/article/10.1007%2Fs12026-015-8737-y
N2  - The role of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) in the exacerbation of inflammatory bowel disease (IBD) is still uncertain. We prospectively investigated the presence of EBV and HCMV infection in both epithelial and immune cells of colonic mucosa of IBD patients, both refractory and responders to standard therapies, in comparison with patients suffering from irritable bowel syndrome who were considered as controls, by using quantitative real-time polymerase chain reaction, immunohistochemistry and in situ hybridization, in an attempt to assess viral localization, DNA load, life cycle phase and possible correlation with disease activity indexes. We obtained clear evidence of the presence of high DNA loads of both viruses in either enterocytes or immune cells of refractory IBD patients, whereas we observed low levels in the responder group and an absence of detectable copies in all cell populations of controls. Remarkably, the values of EBV and HCMV DNA in inflamed mucosa were invariably higher than in non-inflamed areas in both IBD groups, and the EBV DNA loads in the cell populations of diseased mucosa of refractory IBD patients positively correlated with the severity of mucosal damage and clinical indexes of activity. Moreover, EBV infection resulted the most prevalent either alone or in combination with HCMV, while immunohistochemistry and in situ hybridization did not allow us to distinguish between the different phases of viral life cycle. Finally, as regards treatment, these novel findings could pave the way for the use of new antiviral molecules in the treatment of this condition.
ER  -

CICCOCIOPPO, R.; F. RACCA; L. SCUDELLER; A. PIRALLA; P. FORMAGNANA; L. POZZI; E. BETTI; A. VANOLI; R. RIBONI; Peter KRUŽLIAK; F. BALDANTI a G. R. CORAZZA. Differential cellular localization of Epstein-Barr virus and human cytomegalovirus in the colonic mucosa of patients with active or quiescent inflammatory bowel disease. \textit{Immunologic Research}. Totowa: Humana Press, 2016, roč.~64, č.~1, s.~191-203. ISSN~0257-277X. Dostupné z: https://doi.org/10.1007/s12026-015-8737-y.

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