PRINCIPAL COMPONENT ANALYSIS OF RAMAN SPECTROSCOPY DATA FOR
DETERMINATION OF BIOFILM FORMING BACTERIA AND YEASTS

D 2016

PRINCIPAL COMPONENT ANALYSIS OF RAMAN SPECTROSCOPY DATA FOR DETERMINATION OF BIOFILM FORMING BACTERIA AND YEASTS

SILER, M.; O. SAMEK; S. BERNATOVA; K. MLYNARIKOVA; J. JEZEK et al.

Základní údaje

Originální název

PRINCIPAL COMPONENT ANALYSIS OF RAMAN SPECTROSCOPY DATA FOR DETERMINATION OF BIOFILM FORMING BACTERIA AND YEASTS

Autoři

SILER, M.; O. SAMEK; S. BERNATOVA; K. MLYNARIKOVA; J. JEZEK; M. SERY; V. KRZYZANEK; K. HRUBANOVA; Veronika HOLÁ ; Filip RŮŽIČKA a P. ZEMANEK

Vydání

BRNO, Recent Trends in Charged Particle Optics and Surface Physics Instrumentation, od s. 66-67, 2 s. 2016

Nakladatel

ACAD SCIENCES, CZECH REPUBLIC, INST SCIENTIFIC INSTRUMENTS ASCR, V V I

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10306 Optics

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/16:00113814

Organizační jednotka

Lékařská fakulta

ISBN

978-80-87441-17-6

UT WoS

000391254000030

Klíčová slova anglicky

biofilm; electron microscopy; Raman spectra

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 4. 2020 16:17, Mgr. Tereza Miškechová

Anotace

V originále

Many microorganisms (e.g., bacteria, yeast, and algae) are known to form a multi-layered structure composed of cells and extracellular matrix on various types of surfaces. Such a formation is known as the biofilm. Special attention is now paid to bacterial biofilms that are formed on the surface of medical implants, surgical fixations, and artificial tissue/vascular\nreplacements. Cells contained within such a biofilm are well protected against antibiotics and phagocytosis and, thus, effectively resist antimicrobial attack.\nA method for in vitro identification of individual bacterial cells as well as yeast colonies is presented. Figure 1 shows an an example of the biofilm formed by Staphylococcus epidermidis bacteria and Candida parapsilosis yeasts known for forming biofilms. The\npresented method is based on analysis of spectral “Raman fingerprints” obtained from the single cell or whole colony, see figure 2(top). Here, Raman spectra might be taken from the biofilm-forming cells without the influence of an extracellular matrix or directly form the bacterial/yeast colony.

Citovat

SILER, M.; O. SAMEK; S. BERNATOVA; K. MLYNARIKOVA; J. JEZEK; M. SERY; V. KRZYZANEK; K. HRUBANOVA; Veronika HOLÁ; Filip RŮŽIČKA a P. ZEMANEK. PRINCIPAL COMPONENT ANALYSIS OF RAMAN SPECTROSCOPY DATA FOR DETERMINATION OF BIOFILM FORMING BACTERIA AND YEASTS. In Filip Mika. Recent Trends in Charged Particle Optics and Surface Physics Instrumentation. BRNO: ACAD SCIENCES, CZECH REPUBLIC, INST SCIENTIFIC INSTRUMENTS ASCR, V V I, 2016, s. 66-67. ISBN 978-80-87441-17-6.

@inproceedings{1650077,
   author = {Siler, M. and Samek, O. and Bernatova, S. and Mlynarikova, K. and Jezek, J. and Sery, M. and Krzyzanek, V. and Hrubanova, K. and Holá, Veronika and Růžička, Filip and Zemanek, P.},
   address = {BRNO},
   booktitle = {Recent Trends in Charged Particle Optics and Surface Physics Instrumentation},
   editor = {Filip Mika},
   keywords = {biofilm; electron microscopy; Raman spectra},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {BRNO},
   isbn = {978-80-87441-17-6},
   pages = {66-67},
   publisher = {ACAD SCIENCES, CZECH REPUBLIC, INST SCIENTIFIC INSTRUMENTS ASCR, V V I},
   title = {PRINCIPAL COMPONENT ANALYSIS OF RAMAN SPECTROSCOPY DATA FOR DETERMINATION OF BIOFILM FORMING BACTERIA AND YEASTS},
   year = {2016}
}

TY  - CONF
ID  - 1650077
AU  - Siler, M. - Samek, O. - Bernatova, S. - Mlynarikova, K. - Jezek, J. - Sery, M. - Krzyzanek, V. - Hrubanova, K. - Holá, Veronika - Růžička, Filip - Zemanek, P.
PY  - 2016
TI  - PRINCIPAL COMPONENT ANALYSIS OF RAMAN SPECTROSCOPY DATA FOR DETERMINATION OF BIOFILM FORMING BACTERIA AND YEASTS
PB  - ACAD SCIENCES, CZECH REPUBLIC, INST SCIENTIFIC INSTRUMENTS ASCR, V V I
CY  - BRNO
SN  - 9788087441176
KW  - biofilm
KW  - electron microscopy
KW  - Raman spectra
N2  - Many microorganisms (e.g., bacteria, yeast, and algae) are known to form a multi-layered structure composed of cells and extracellular matrix on various types of surfaces. Such a formation is known as the biofilm. Special attention is now paid to bacterial biofilms that are formed on the surface of medical implants, surgical fixations, and artificial tissue/vascular\nreplacements. Cells contained within such a biofilm are well protected against antibiotics and phagocytosis and, thus, effectively resist antimicrobial attack.\nA method for in vitro identification of individual bacterial cells as well as yeast colonies is presented. Figure 1 shows an an example of the biofilm formed by Staphylococcus epidermidis bacteria and Candida parapsilosis yeasts known for forming biofilms. The\npresented method is based on analysis of spectral “Raman fingerprints” obtained from the single cell or whole colony, see figure 2(top). Here, Raman spectra might be taken from the biofilm-forming cells without the influence of an extracellular matrix or directly form the bacterial/yeast colony.
ER  -

SILER, M.; O. SAMEK; S. BERNATOVA; K. MLYNARIKOVA; J. JEZEK; M. SERY; V. KRZYZANEK; K. HRUBANOVA; Veronika HOLÁ; Filip RŮŽIČKA a P. ZEMANEK. PRINCIPAL COMPONENT ANALYSIS OF RAMAN SPECTROSCOPY DATA FOR DETERMINATION OF BIOFILM FORMING BACTERIA AND YEASTS. In Filip Mika. \textit{Recent Trends in Charged Particle Optics and Surface Physics Instrumentation}. BRNO: ACAD SCIENCES, CZECH REPUBLIC, INST SCIENTIFIC INSTRUMENTS ASCR, V V I, 2016, s.~66-67. ISBN~978-80-87441-17-6.

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