Prevalence of bacteriocins and their co-association with
virulence factors within Pseudomonas aeruginosa catheter
isolates

J 2020

Prevalence of bacteriocins and their co-association with virulence factors within Pseudomonas aeruginosa catheter isolates

SNOPKOVÁ, Kateřina; Kristýna DUFKOVÁ; Petra KLIMEŠOVÁ; Martina VANĚRKOVÁ; Filip RŮŽIČKA et al.

Základní údaje

Originální název

Prevalence of bacteriocins and their co-association with virulence factors within Pseudomonas aeruginosa catheter isolates

Autoři

SNOPKOVÁ, Kateřina; Kristýna DUFKOVÁ; Petra KLIMEŠOVÁ; Martina VANĚRKOVÁ; Filip RŮŽIČKA a Veronika HOLÁ

Vydání

International Journal of Medical Microbiology, Munich, Elsevier, 2020, 1438-4221

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.473

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/20:00118599

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Bacteriocin; Pyocin; Pseudomonas aeruginosa; CAUTI; Siderophores

Štítky

14110113, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 1. 2021 10:12, Mgr. Tereza Miškechová

Anotace

V originále

Urinary tract infections represent common nosocomial infectious diseases. Bacteriocin production has been recently described as a putative virulence factor in these infections but studies focusing particularly on Pseudomonas aeruginosa are not available. Therefore, we assessed the prevalence of the bacteriocin genes, their co-occurrence and their co-association with previously detected virulence factors in a set of 135 P. aeruginosa strains from catheter-associated urinary tract infections (CAUTIs). The overall bacteriocinogeny reached 96.3 % with an average of 3.6 genes per strain. The most frequently detected determinants were the encoded pyocins 54 (76.3 %), R (69.6 %), and 52 (67.4 %). A statistically significant co-occurrence and a negative relationship were observed between several pyocin types. Particular pyocins exhibited associations with biofilm formation, production of pyochelin, pyocyanin, antibiotic-degrading enzymes, overall strain susceptibility and resistance, and motility of the strain. Co-occurrence of the pyocins 52 and 54 (p<<0.0001; Z = 13.15), both utilizating the ferripyoverdine receptor FpvAI, was found but no relation to pyoverdine production was detected. A negative association (p = 0.0047; Z=-2.83) was observed between pyochelin and pyocin 55 utilising the ferripyochelin receptor FptA. Pairwise assays resulted in 52.1 % inhibition which was equally distributed between soluble and particle types of antimicrobials. In conclusion, pyocin determinants appear to be important characteristics of CAUTI-related P. aeruginosa isolates and could contribute to their urovirulence.

Návaznosti

NV16-31593A, projekt VaV

Název: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí

Přiložené soubory

pairwise_assay_pyocins_R.xlsx

Data_FINAL_Canoco.xls

Citovat

SNOPKOVÁ, Kateřina; Kristýna DUFKOVÁ; Petra KLIMEŠOVÁ; Martina VANĚRKOVÁ; Filip RŮŽIČKA a Veronika HOLÁ. Prevalence of bacteriocins and their co-association with virulence factors within Pseudomonas aeruginosa catheter isolates. International Journal of Medical Microbiology. Munich: Elsevier, 2020, roč. 310, č. 8, s. 1-7. ISSN 1438-4221. Dostupné z: https://doi.org/10.1016/j.ijmm.2020.151454.

@article{1652256,
   author = {Snopková, Kateřina and Dufková, Kristýna and Klimešová, Petra and Vaněrková, Martina and Růžička, Filip and Holá, Veronika},
   article_location = {Munich},
   article_number = {8},
   doi = {https://doi.org/10.1016/j.ijmm.2020.151454},
   keywords = {Bacteriocin; Pyocin; Pseudomonas aeruginosa; CAUTI; Siderophores},
   language = {eng},
   issn = {1438-4221},
   journal = {International Journal of Medical Microbiology},
   title = {Prevalence of bacteriocins and their co-association with virulence factors within Pseudomonas aeruginosa catheter isolates},
   url = {https://www.sciencedirect.com/science/article/pii/S1438422120300643?via%3Dihub#!},
   volume = {310},
   year = {2020}
}

TY  - JOUR
ID  - 1652256
AU  - Snopková, Kateřina - Dufková, Kristýna - Klimešová, Petra - Vaněrková, Martina - Růžička, Filip - Holá, Veronika
PY  - 2020
TI  - Prevalence of bacteriocins and their co-association with virulence factors within Pseudomonas aeruginosa catheter isolates
JF  - International Journal of Medical Microbiology
VL  - 310
IS  - 8
SP  - 1-7
EP  - 1-7
PB  - Elsevier
SN  - 14384221
KW  - Bacteriocin
KW  - Pyocin
KW  - Pseudomonas aeruginosa
KW  - CAUTI
KW  - Siderophores
UR  - https://www.sciencedirect.com/science/article/pii/S1438422120300643?via%3Dihub#!
L2  - https://www.sciencedirect.com/science/article/pii/S1438422120300643?via%3Dihub#!
N2  - Urinary tract infections represent common nosocomial infectious diseases. Bacteriocin production has been recently described as a putative virulence factor in these infections but studies focusing particularly on Pseudomonas aeruginosa are not available. Therefore, we assessed the prevalence of the bacteriocin genes, their co-occurrence and their co-association with previously detected virulence factors in a set of 135 P. aeruginosa strains from catheter-associated urinary tract infections (CAUTIs). The overall bacteriocinogeny reached 96.3 % with an average of 3.6 genes per strain. The most frequently detected determinants were the encoded pyocins 54 (76.3 %), R (69.6 %), and 52 (67.4 %). A statistically significant co-occurrence and a negative relationship were observed between several pyocin types. Particular pyocins exhibited associations with biofilm formation, production of pyochelin, pyocyanin, antibiotic-degrading enzymes, overall strain susceptibility and resistance, and motility of the strain. Co-occurrence of the pyocins 52 and 54 (p<<0.0001; Z = 13.15), both utilizating the ferripyoverdine receptor FpvAI, was found but no relation to pyoverdine production was detected. A negative association (p = 0.0047; Z=-2.83) was observed between pyochelin and pyocin 55 utilising the ferripyochelin receptor FptA. Pairwise assays resulted in 52.1 % inhibition which was equally distributed between soluble and particle types of antimicrobials. In conclusion, pyocin determinants appear to be important characteristics of CAUTI-related P. aeruginosa isolates and could contribute to their urovirulence.
ER  -

SNOPKOVÁ, Kateřina; Kristýna DUFKOVÁ; Petra KLIMEŠOVÁ; Martina VANĚRKOVÁ; Filip RŮŽIČKA a Veronika HOLÁ. Prevalence of bacteriocins and their co-association with virulence factors within Pseudomonas aeruginosa catheter isolates. \textit{International Journal of Medical Microbiology}. Munich: Elsevier, 2020, roč.~310, č.~8, s.~1-7. ISSN~1438-4221. Dostupné z: https://doi.org/10.1016/j.ijmm.2020.151454.

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