PRUCHA, J., J. SKOPALIK, Ivan JUSTAN, T. PARAK, E. GABRIELOVA, K. HANA a L. NAVRATIL. High Inductive Magnetic Stimuli and Their Effects on Mesenchymal Stromal Cells, Dendritic Cells, and Fibroblasts. Physiological research. Praha: Fyziologický ústav AV ČR, roč. 68, Supplement 4, s. "S433"-"S443", 11 s. ISSN 0862-8408. doi:10.33549/physiolres.934382. 2019.
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Základní údaje
Originální název High Inductive Magnetic Stimuli and Their Effects on Mesenchymal Stromal Cells, Dendritic Cells, and Fibroblasts
Autoři PRUCHA, J. (203 Česká republika), J. SKOPALIK (203 Česká republika, garant), Ivan JUSTAN (203 Česká republika, domácí), T. PARAK (203 Česká republika), E. GABRIELOVA (203 Česká republika), K. HANA (203 Česká republika) a L. NAVRATIL (203 Česká republika).
Vydání Physiological research, Praha, Fyziologický ústav AV ČR, 2019, 0862-8408.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 30105 Physiology
Stát vydavatele Česká republika
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 1.655
Kód RIV RIV/00216224:14110/19:00115641
Organizační jednotka Lékařská fakulta
Doi http://dx.doi.org/10.33549/physiolres.934382
UT WoS 000529413800009
Klíčová slova anglicky Electromagnetic stimulation; Cell migration; Mesenchymal stromal cells; Fibroblasts; Dendritic cells
Štítky 14110813, rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Tereza Miškechová, učo 341652. Změněno: 15. 5. 2020 14:39.
Anotace
Effects of low-frequency electromagnetic fields (LF EMF) on the activation of different tissue recovery processes have already been fully understood. Preliminary recent data demonstrated that a special case of sinusoidal electromagnetic fields, known as amplitude-modulated currents (AMC) could have a potential to accelerate the cell metabolism or cell migration. An AMC generator was designed to generate sinusoidal induced electric currents with the amplitude modulation and the harmonic carrier frequency of 5,000 Hz was modulated by frequencies of 1 to 100 Hz. The magnetic field peak was 6 mT, electric field intensity 2 V/m and the current density of induced electrical currents was approximately 1 A/m(2). The coil of the generator was adapted to easy handling and safe integration into the shelf of the CO2 incubator. The shelf with the coil was prepared for the introduction of cells in standard plastic in vitro chambers. The tests focused on cells with migratory capacity after injury or during immunological processes and thus, mesenchymal stromal cells (MSC), dendritic cells (DC), and fibroblasts were chosen. The tests involved exposures of the cells to LF EMF (180 min/day) every day, for a period of three days, before examining them for cell death, morphology changes, and CD markers. The samples were tested by using MTT assay and the effects on the intracellular concentration of reactive oxygen species were quantified. The cell migration was finally measured with the help of the transwell migration assay. None of the cell types showed any decrease in the cell viability after the LF EMF application and the cells displayed minimum changes in reactive oxygen species. Functional changes (acceleration of cell migration) after AMC exposure were statistically significant for the MSC samples only. The acceleration of MSCs is associated with the production of MMP by these cells. The EMF has a potential to be a safe, clinically applicable selective activator of MSC homing, MSC paracrine production, and subsequent regeneration processes.
VytisknoutZobrazeno: 28. 3. 2024 10:07