Fully Automated Spectrometric Protocols for Determination of
Antioxidant Activity: Advantages and Disadvantages

J 2010

Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages

SOCHOR, Jiri, Marketa RYVOLOVA, Olga KRYSTOFOVA, Petr SALAS, Jaromir HUBALEK et. al.

Základní údaje

Originální název

Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages

Autoři

SOCHOR, Jiri, Marketa RYVOLOVA, Olga KRYSTOFOVA, Petr SALAS, Jaromir HUBALEK, Vojtech ADAM, Libuše TRNKOVÁ (203 Česká republika, domácí), Ladislav HAVEL, Miroslava BEKLOVÁ (203 Česká republika), Josef ZEHNALEK, Ivo PROVAZNIK a René KIZEK (203 Česká republika, garant)

Vydání

Blood Cells, Molecules and Diseases, Orlando, Florida, Academic Press, 2010, 1079-9796

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.716

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/molecules15128618

UT WoS

000285709000008

Klíčová slova anglicky

antioxidant activity; spectrometry; trolox; gallic acid

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 5. 2020 14:47, Mgr. Marie Šípková, DiS.

Anotace

V originále

The aim of this study was to describe behaviour, kinetics, time courses and limitations of the six different fully automated spectrometric methods - DPPH, TEAC, FRAP, DMPD, Free Radicals and Blue CrO(5). Absorption curves were measured and absorbance maxima were found. All methods were calibrated using the standard compounds Trolox (R) and/or gallic acid. Calibration curves were determined (relative standard deviation was within the range from 1.5 to 2.5 %). The obtained characteristics were compared and discussed. Moreover, the data obtained were applied to optimize and to automate all mentioned protocols. Automatic analyzer allowed us to analyse simultaneously larger set of samples, to decrease the measurement time, to eliminate the errors and to provide data of higher quality in comparison to manual analysis. The total time of analysis for one sample was decreased to 10 min for all six methods. In contrary, the total time of manual spectrometric determination was approximately 120 min. The obtained data provided good correlations between studied methods (R = 0.97 - 0.99).

Návaznosti

GD102/09/H083, projekt VaV

Název: Informační technologie v biomedicínském inženýrství

Investor: Grantová agentura ČR, Informační technologie v biomedicínckém inženýrství

Citovat

SOCHOR, Jiri, Marketa RYVOLOVA, Olga KRYSTOFOVA, Petr SALAS, Jaromir HUBALEK, Vojtech ADAM, Libuše TRNKOVÁ, Ladislav HAVEL, Miroslava BEKLOVÁ, Josef ZEHNALEK, Ivo PROVAZNIK a René KIZEK. Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages. Blood Cells, Molecules and Diseases. Orlando, Florida: Academic Press, 2010, roč. 15, č. 12, s. 8618-8640. ISSN 1079-9796. Dostupné z: https://dx.doi.org/10.3390/molecules15128618.

@article{1658018,
   author = {Sochor, Jiri and Ryvolova, Marketa and Krystofova, Olga and Salas, Petr and Hubalek, Jaromir and Adam, Vojtech and Trnková, Libuše and Havel, Ladislav and Beklová, Miroslava and Zehnalek, Josef and Provaznik, Ivo and Kizek, René},
   article_location = {Orlando, Florida},
   article_number = {12},
   doi = {http://dx.doi.org/10.3390/molecules15128618},
   keywords = {antioxidant activity; spectrometry; trolox; gallic acid},
   language = {eng},
   issn = {1079-9796},
   journal = {Blood Cells, Molecules and Diseases},
   title = {Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages},
   url = {https://www.mdpi.com/1420-3049/15/12/8618},
   volume = {15},
   year = {2010}
}

TY  - JOUR
ID  - 1658018
AU  - Sochor, Jiri - Ryvolova, Marketa - Krystofova, Olga - Salas, Petr - Hubalek, Jaromir - Adam, Vojtech - Trnková, Libuše - Havel, Ladislav - Beklová, Miroslava - Zehnalek, Josef - Provaznik, Ivo - Kizek, René
PY  - 2010
TI  - Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages
JF  - Blood Cells, Molecules and Diseases
VL  - 15
IS  - 12
SP  - 8618-8640
EP  - 8618-8640
PB  - Academic Press
SN  - 10799796
KW  - antioxidant activity
KW  - spectrometry
KW  - trolox
KW  - gallic acid
UR  - https://www.mdpi.com/1420-3049/15/12/8618
L2  - https://www.mdpi.com/1420-3049/15/12/8618
N2  - The aim of this study was to describe behaviour, kinetics, time courses and limitations of the six different fully automated spectrometric methods - DPPH, TEAC, FRAP, DMPD, Free Radicals and Blue CrO(5). Absorption curves were measured and absorbance maxima were found. All methods were calibrated using the standard compounds Trolox (R) and/or gallic acid. Calibration curves were determined (relative standard deviation was within the range from 1.5 to 2.5 %). The obtained characteristics were compared and discussed. Moreover, the data obtained were applied to optimize and to automate all mentioned protocols. Automatic analyzer allowed us to analyse simultaneously larger set of samples, to decrease the measurement time, to eliminate the errors and to provide data of higher quality in comparison to manual analysis. The total time of analysis for one sample was decreased to 10 min for all six methods. In contrary, the total time of manual spectrometric determination was approximately 120 min. The obtained data provided good correlations between studied methods (R = 0.97 - 0.99).
ER  -

SOCHOR, Jiri, Marketa RYVOLOVA, Olga KRYSTOFOVA, Petr SALAS, Jaromir HUBALEK, Vojtech ADAM, Libuše TRNKOVÁ, Ladislav HAVEL, Miroslava BEKLOVÁ, Josef ZEHNALEK, Ivo PROVAZNIK a René KIZEK. Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages. \textit{Blood Cells, Molecules and Diseases}. Orlando, Florida: Academic Press, 2010, roč.~15, č.~12, s.~8618-8640. ISSN~1079-9796. Dostupné z: https://dx.doi.org/10.3390/molecules15128618.

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