Effects of Reduced Glutathione, Surface Active Agents, and
Ionic Strength on the Detection of Metallothioneins by Using of
Brdicka Reaction

J 2009

Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction

KRIZKOVA, Sona; Ivo FABRIK; Vojtech ADAM; Jiri KUKACKA; Richard PRUSA et al.

Základní údaje

Originální název

Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction

Autoři

KRIZKOVA, Sona; Ivo FABRIK; Vojtech ADAM; Jiri KUKACKA; Richard PRUSA; Libuše TRNKOVÁ; Jan STRNADEL; Vratislav HORAK a Rene KIZEK

Vydání

Electroanalysis, WILEY-V C H VERLAG GMBH, 2009, 1040-0397

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.630

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Differential pulse voltammetry; Adsorptive transfer stripping technique; Proteins; Metallothionein; Brdicka reaction

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 6. 2020 08:49, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

We investigated the effects of two surface active agents (sodium dodecyl sulfate, SDS, and polyoxyethylene sorbitol, Tween-20), ionic strength and reduced glutathione (GSH) on MT signals measured by adsorptive transfer stripping differential pulse voltammetry Brdicka reaction. If we compared the GSH and MT signals at the accumulation time of 120 s, the MT signal was more than two hundred times higher than the GSH one. The influence of Tween-20 was investigated in a broad concentration range (from 10(-8) to 10(-11) %, v/v). We found that the catalytic Cat2 signal decreased for more than 60% with increasing Tween-20 content. In the case of using SDS (10(-3) - 10(-6) M), the increase of Cat2 peak in the presence of the highest SDS concentration was determined. The height of Cat2 peak enhanced with increasing ionic strength mimicking by NaCl concentration. Based on the enhancement of the Cat2 peak we determined the ionic strength of fibroblasts sample and recalculated the concentration of MT determined there.

Citovat

KRIZKOVA, Sona; Ivo FABRIK; Vojtech ADAM; Jiri KUKACKA; Richard PRUSA; Libuše TRNKOVÁ; Jan STRNADEL; Vratislav HORAK a Rene KIZEK. Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction. Electroanalysis. WILEY-V C H VERLAG GMBH, 2009, roč. 21, 3-5, s. 640-644. ISSN 1040-0397. Dostupné z: https://doi.org/10.1002/elan.200804406.

@article{1662579,
   author = {Krizkova, Sona and Fabrik, Ivo and Adam, Vojtech and Kukacka, Jiri and Prusa, Richard and Trnková, Libuše and Strnadel, Jan and Horak, Vratislav and Kizek, Rene},
   article_number = {3-5},
   doi = {https://doi.org/10.1002/elan.200804406},
   keywords = {Differential pulse voltammetry; Adsorptive transfer stripping technique; Proteins; Metallothionein; Brdicka reaction},
   language = {eng},
   issn = {1040-0397},
   journal = {Electroanalysis},
   title = {Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction},
   url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.200804406},
   volume = {21},
   year = {2009}
}

TY  - JOUR
ID  - 1662579
AU  - Krizkova, Sona - Fabrik, Ivo - Adam, Vojtech - Kukacka, Jiri - Prusa, Richard - Trnková, Libuše - Strnadel, Jan - Horak, Vratislav - Kizek, Rene
PY  - 2009
TI  - Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction
JF  - Electroanalysis
VL  - 21
IS  - 3-5
SP  - 640-644
EP  - 640-644
PB  - WILEY-V C H VERLAG GMBH
SN  - 10400397
KW  - Differential pulse voltammetry
KW  - Adsorptive transfer stripping technique
KW  - Proteins
KW  - Metallothionein
KW  - Brdicka reaction
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.200804406
L2  - https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.200804406
N2  - We investigated the effects of two surface active agents (sodium dodecyl sulfate, SDS, and polyoxyethylene sorbitol, Tween-20), ionic strength and reduced glutathione (GSH) on MT signals measured by adsorptive transfer stripping differential pulse voltammetry Brdicka reaction. If we compared the GSH and MT signals at the accumulation time of 120 s, the MT signal was more than two hundred times higher than the GSH one. The influence of Tween-20 was investigated in a broad concentration range (from 10(-8) to 10(-11) %, v/v). We found that the catalytic Cat2 signal decreased for more than 60% with increasing Tween-20 content. In the case of using SDS (10(-3) - 10(-6) M), the increase of Cat2 peak in the presence of the highest SDS concentration was determined. The height of Cat2 peak enhanced with increasing ionic strength mimicking by NaCl concentration. Based on the enhancement of the Cat2 peak we determined the ionic strength of fibroblasts sample and recalculated the concentration of MT determined there.
ER  -

KRIZKOVA, Sona; Ivo FABRIK; Vojtech ADAM; Jiri KUKACKA; Richard PRUSA; Libuše TRNKOVÁ; Jan STRNADEL; Vratislav HORAK a Rene KIZEK. Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction. \textit{Electroanalysis}. WILEY-V C H VERLAG GMBH, 2009, roč.~21, 3-5, s.~640-644. ISSN~1040-0397. Dostupné z: https://doi.org/10.1002/elan.200804406.

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