Adenosine-5-Phosphosulfate- and Sulfite Reductases Activities
of Sulfate-Reducing Bacteria from Various Environments

J 2020

Adenosine-5-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments

KUSHKEVYCH, Ivan, Daryna ABDULINA, Jozef KOVÁČ, Dani DORDEVIĆ, Monika VÍTĚZOVÁ et. al.

Základní údaje

Originální název

Adenosine-5-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments

Autoři

KUSHKEVYCH, Ivan (804 Ukrajina, garant, domácí), Daryna ABDULINA (804 Ukrajina), Jozef KOVÁČ (703 Slovensko, domácí), Dani DORDEVIĆ (203 Česká republika), Monika VÍTĚZOVÁ (203 Česká republika, domácí), Galyna IUTYNSKA (804 Ukrajina) a Simon K.-M. R. RITTMANN (40 Rakousko)

Vydání

Biomolecules, Basel, MDPI, 2020, 2218-273X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.879

Kód RIV

RIV/00216224:14310/20:00115814

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/biom10060921

UT WoS

000554651800001

Klíčová slova anglicky

hydrogen sulfide; toxicity; sulfate-reducing bacteria; cell-free extracts; ecotopes

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 10. 2020 13:50, Mgr. Marie Šípková, DiS.

Anotace

V originále

A comparative study of the kinetic characteristics (specific activity, initial and maximum rate, and affinity for substrates) of key enzymes of assimilatory sulfate reduction (APS reductase and dissimilatory sulfite reductase) in cell-free extracts of sulphate-reducing bacteria (SRB) from various biotopes was performed. The material for the study represented different strains of SRB from various ecotopes. Microbiological (isolation and cultivation), biochemical (free cell extract preparation) and chemical (enzyme activity determination) methods served in defining kinetic characteristics of SRB enzymes. The determined affinity data for substrates (i.e., sulfite) were 10 times higher for SRB strains isolated from environmental (soil) ecotopes than for strains from the human intestine.

Návaznosti

MUNI/A/0947/2019, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 4 (Akronym: SV2020)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 4, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

KUSHKEVYCH, Ivan, Daryna ABDULINA, Jozef KOVÁČ, Dani DORDEVIĆ, Monika VÍTĚZOVÁ, Galyna IUTYNSKA a Simon K.-M. R. RITTMANN. Adenosine-5-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments. Biomolecules. Basel: MDPI, 2020, roč. 10, č. 6, s. 1-15. ISSN 2218-273X. Dostupné z: https://dx.doi.org/10.3390/biom10060921.

@article{1662922,
   author = {Kushkevych, Ivan and Abdulina, Daryna and Kováč, Jozef and Dordević, Dani and Vítězová, Monika and Iutynska, Galyna and Rittmann, Simon K.andM. R.},
   article_location = {Basel},
   article_number = {6},
   doi = {http://dx.doi.org/10.3390/biom10060921},
   keywords = {hydrogen sulfide; toxicity; sulfate-reducing bacteria; cell-free extracts; ecotopes},
   language = {eng},
   issn = {2218-273X},
   journal = {Biomolecules},
   title = {Adenosine-5-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments},
   url = {https://www.mdpi.com/2218-273X/10/6/921},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1662922
AU  - Kushkevych, Ivan - Abdulina, Daryna - Kováč, Jozef - Dordević, Dani - Vítězová, Monika - Iutynska, Galyna - Rittmann, Simon K.-M. R.
PY  - 2020
TI  - Adenosine-5-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments
JF  - Biomolecules
VL  - 10
IS  - 6
SP  - 1-15
EP  - 1-15
PB  - MDPI
SN  - 2218273X
KW  - hydrogen sulfide
KW  - toxicity
KW  - sulfate-reducing bacteria
KW  - cell-free extracts
KW  - ecotopes
UR  - https://www.mdpi.com/2218-273X/10/6/921
L2  - https://www.mdpi.com/2218-273X/10/6/921
N2  - A comparative study of the kinetic characteristics (specific activity, initial and maximum rate, and affinity for substrates) of key enzymes of assimilatory sulfate reduction (APS reductase and dissimilatory sulfite reductase) in cell-free extracts of sulphate-reducing bacteria (SRB) from various biotopes was performed. The material for the study represented different strains of SRB from various ecotopes. Microbiological (isolation and cultivation), biochemical (free cell extract preparation) and chemical (enzyme activity determination) methods served in defining kinetic characteristics of SRB enzymes. The determined affinity data for substrates (i.e., sulfite) were 10 times higher for SRB strains isolated from environmental (soil) ecotopes than for strains from the human intestine.
ER  -

KUSHKEVYCH, Ivan, Daryna ABDULINA, Jozef KOVÁČ, Dani DORDEVI$\backslash$'C, Monika VÍTĚZOVÁ, Galyna IUTYNSKA a Simon K.-M. R. RITTMANN. Adenosine-5-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments. \textit{Biomolecules}. Basel: MDPI, 2020, roč.~10, č.~6, s.~1-15. ISSN~2218-273X. Dostupné z: https://dx.doi.org/10.3390/biom10060921.

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