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@article{1663988,
author = {Farka, Zdeněk and Mickert, Matthias Jürgen and Pastucha, Matěj and Mikušová, Zuzana and Skládal, Petr and Gorris, HansandHeiner},
article_location = {Weinheim},
article_number = {27},
doi = {http://dx.doi.org/10.1002/anie.201913924},
keywords = {digital assays; immunoassays; optical detection; signal background; single-molecule detection},
language = {eng},
issn = {1433-7851},
journal = {Angewandte Chemie International Edition},
title = {Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays},
url = {https://onlinelibrary.wiley.com/doi/10.1002/anie.201913924},
volume = {59},
year = {2020}
}
TY - JOUR
ID - 1663988
AU - Farka, Zdeněk - Mickert, Matthias Jürgen - Pastucha, Matěj - Mikušová, Zuzana - Skládal, Petr - Gorris, Hans-Heiner
PY - 2020
TI - Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays
JF - Angewandte Chemie International Edition
VL - 59
IS - 27
SP - 10746-10773
EP - 10746-10773
PB - Wiley-VCH
SN - 14337851
KW - digital assays
KW - immunoassays
KW - optical detection
KW - signal background
KW - single-molecule detection
UR - https://onlinelibrary.wiley.com/doi/10.1002/anie.201913924
L2 - https://onlinelibrary.wiley.com/doi/10.1002/anie.201913924
N2 - The ability to detect low concentrations of analytes and in particular low-abundance biomarkers is of fundamental importance, e.g., for early-stage disease diagnosis. The prospect of reaching the ultimate limit of detection has driven the development of single-molecule bioaffinity assays. While many review articles have highlighted the potentials of single-molecule technologies for analytical and diagnostic applications, these technologies are not as widespread in real-world applications as one should expect. This Review provides a theoretical background on single-molecule—or better digital—assays to critically assess their potential compared to traditional analog assays. Selected examples from the literature include bioaffinity assays for the detection of biomolecules such as proteins, nucleic acids, and viruses. The structure of the Review highlights the versatility of optical single-molecule labeling techniques, including enzymatic amplification, molecular labels, and innovative nanomaterials.
ER -
FARKA, Zdeněk, Matthias Jürgen MICKERT, Matěj PASTUCHA, Zuzana MIKUŠOVÁ, Petr SKLÁDAL and Hans-Heiner GORRIS. Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays. \textit{Angewandte Chemie International Edition}. Weinheim: Wiley-VCH, 2020, vol.~59, No~27, p.~10746-10773. ISSN~1433-7851. Available from: https://dx.doi.org/10.1002/anie.201913924.
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