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@article{1664947,
author = {Křemenová, Jana and Balvín, Ondřej and Otti, Oliver and Pavonič, Michal and Reinhardt, Klaus and Šimek, Zdeněk and Bartonička, Tomáš},
article_location = {London},
article_number = {1},
doi = {http://dx.doi.org/10.1038/s41598-020-66919-5},
keywords = {fluorescent pigments; haematophagy; method optimization; insect; age-grading; LC-MS/MS},
language = {eng},
issn = {2045-2322},
journal = {Scientific reports},
title = {Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry},
url = {https://www.nature.com/articles/s41598-020-66919-5#citeas},
volume = {10},
year = {2020}
}
TY - JOUR
ID - 1664947
AU - Křemenová, Jana - Balvín, Ondřej - Otti, Oliver - Pavonič, Michal - Reinhardt, Klaus - Šimek, Zdeněk - Bartonička, Tomáš
PY - 2020
TI - Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry
JF - Scientific reports
VL - 10
IS - 1
SP - 1-14
EP - 1-14
PB - Nature Publishing Group
SN - 20452322
KW - fluorescent pigments
KW - haematophagy
KW - method optimization
KW - insect
KW - age-grading
KW - LC-MS/MS
UR - https://www.nature.com/articles/s41598-020-66919-5#citeas
L2 - https://www.nature.com/articles/s41598-020-66919-5#citeas
N2 - Determining the age of free-living insects, particularly of blood-sucking species, is important for human health because such knowledge critically influences the estimates of biting frequency and vectoring ability. Genetic age determination is currently not available. Pteridines gradually accumulate in the eyes of insects and their concentrations is the prevailing method. Despite of their stability, published extractions differ considerably, including for standards, for mixtures of pteridines and even for light conditions. This methodological inconsistency among studies is likely to influence age estimates severely and to hamper their comparability. Therefore we reviewed methodological steps across 106 studies to identify methodological denominators and results across studies. Second, we experimentally test how different pteridines vary in their age calibration curves in, common bed (Cimex lectularius) and bat bugs (C. pipistrelli). Here we show that the accumulation of particular pteridines varied between a) different populations and b) rearing temperatures but not c) with the impact of light conditions during extraction or d) the type of blood consumed by the bugs. To optimize the extraction of pteridines and measuring concentrations, we recommend the simultaneous measurement of more than one standard and subsequently to select those that show consistent changes over time to differentiate among age cohorts.
ER -
KŘEMENOVÁ, Jana, Ondřej BALVÍN, Oliver OTTI, Michal PAVONIČ, Klaus REINHARDT, Zdeněk ŠIMEK a Tomáš BARTONIČKA. Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry. \textit{Scientific reports}. London: Nature Publishing Group, 2020, roč.~10, č.~1, s.~1-14. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-020-66919-5.
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