A device for investigation of natural cell mobility and
deformability

J 2020

A device for investigation of natural cell mobility and deformability

LEDVINA, Vojtěch, Karel KLEPÁRNÍK, Soňa LEGARTOVÁ a Eva BÁRTOVÁ

Základní údaje

Originální název

A device for investigation of natural cell mobility and deformability

Autoři

LEDVINA, Vojtěch (203 Česká republika, domácí), Karel KLEPÁRNÍK (203 Česká republika, garant), Soňa LEGARTOVÁ (203 Česká republika) a Eva BÁRTOVÁ (203 Česká republika)

Vydání

Electrophoresis, Hoboken, Wiley, 2020, 0173-0835

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.535

Kód RIV

RIV/00216224:14310/20:00115871

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1002/elps.201900357

UT WoS

000533421500001

Klíčová slova anglicky

Cell deformability; Cell mobility; Confocal microscopy; Metastasis; Microfluidics

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 11. 2020 15:41, Mgr. Marie Šípková, DiS.

Anotace

V originále

A microfluidic device made of polydimethylsiloxane was developed for continuous evaluation of natural migration mobility of many eukaryotic cells in relaxed and deformed state. The device was fabricated by standard photolithography and soft lithography techniques using the SU-8 3010 negative photoresist on a glass wafer as the master mold. The simple flow-free device exploits the chemotactic movement of cells through a set of mechanical barriers in the direction of concentration gradients of attractants. The barriers are formed by arrays of circular cross-section pillars with decreasing spacing 7, 5, and 3 mu m. To pass through the obstacles, the cells are deformed and change their cytoskeletal architecture. The instantaneous migration velocities of cells are monitored in a time-lapse setup of the scanning confocal microscope. Thus, the cellular deformability and migratory activity can easily be evaluated. The functionality of the device was tested with model HeLa cells stably transfected with fluorescent Premo FUCCI Cell Cycle Sensor. The designed device has the potential to be implemented for testing the tendency of patients’ tumors to metastasis.

Citovat

LEDVINA, Vojtěch, Karel KLEPÁRNÍK, Soňa LEGARTOVÁ a Eva BÁRTOVÁ. A device for investigation of natural cell mobility and deformability. Electrophoresis. Hoboken: Wiley, 2020, roč. 41, 13-14, s. 1238-1244. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201900357.

@article{1668300,
   author = {Ledvina, Vojtěch and Klepárník, Karel and Legartová, Soňa and Bártová, Eva},
   article_location = {Hoboken},
   article_number = {13-14},
   doi = {http://dx.doi.org/10.1002/elps.201900357},
   keywords = {Cell deformability; Cell mobility; Confocal microscopy; Metastasis; Microfluidics},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {A device for investigation of natural cell mobility and deformability},
   url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900357},
   volume = {41},
   year = {2020}
}

TY  - JOUR
ID  - 1668300
AU  - Ledvina, Vojtěch - Klepárník, Karel - Legartová, Soňa - Bártová, Eva
PY  - 2020
TI  - A device for investigation of natural cell mobility and deformability
JF  - Electrophoresis
VL  - 41
IS  - 13-14
SP  - 1238-1244
EP  - 1238-1244
PB  - Wiley
SN  - 01730835
KW  - Cell deformability
KW  - Cell mobility
KW  - Confocal microscopy
KW  - Metastasis
KW  - Microfluidics
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900357
L2  - https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900357
N2  - A microfluidic device made of polydimethylsiloxane was developed for continuous evaluation of natural migration mobility of many eukaryotic cells in relaxed and deformed state. The device was fabricated by standard photolithography and soft lithography techniques using the SU-8 3010 negative photoresist on a glass wafer as the master mold. The simple flow-free device exploits the chemotactic movement of cells through a set of mechanical barriers in the direction of concentration gradients of attractants. The barriers are formed by arrays of circular cross-section pillars with decreasing spacing 7, 5, and 3 mu m. To pass through the obstacles, the cells are deformed and change their cytoskeletal architecture. The instantaneous migration velocities of cells are monitored in a time-lapse setup of the scanning confocal microscope. Thus, the cellular deformability and migratory activity can easily be evaluated. The functionality of the device was tested with model HeLa cells stably transfected with fluorescent Premo FUCCI Cell Cycle Sensor. The designed device has the potential to be implemented for testing the tendency of patients’ tumors to metastasis.
ER  -

LEDVINA, Vojtěch, Karel KLEPÁRNÍK, Soňa LEGARTOVÁ a Eva BÁRTOVÁ. A device for investigation of natural cell mobility and deformability. \textit{Electrophoresis}. Hoboken: Wiley, 2020, roč.~41, 13-14, s.~1238-1244. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201900357.

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