Osteogenic impact of pro-apoptotic caspase inhibitors in
MC3T3-E1 cells

J 2020

Osteogenic impact of pro-apoptotic caspase inhibitors in MC3T3-E1 cells

KRATOCHVÍLOVÁ, Adéla; Barbora VESELÁ; Vojtěch LEDVINA; Eva ŠVANDOVÁ; Karel KLEPÁRNÍK et al.

Základní údaje

Originální název

Osteogenic impact of pro-apoptotic caspase inhibitors in MC3T3-E1 cells

Autoři

KRATOCHVÍLOVÁ, Adéla; Barbora VESELÁ; Vojtěch LEDVINA; Eva ŠVANDOVÁ ; Karel KLEPÁRNÍK; Kateřina DADÁKOVÁ ; Petr BENEŠ a Eva MATALOVÁ

Vydání

Scientific Reports, London, Nature Publishing Group, 2020, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10605 Developmental biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.380

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/20:00114208

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Bone; Bone development; Cell biology; Proteases

Štítky

14313010, 14313050, 14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 2. 2023 13:41, prof. Mgr. Petr Beneš, Ph.D.

Anotace

V originále

Caspases are proteases traditionally associated with inflammation and cell death. Recently, they have also been shown to modulate cell proliferation and differentiation. The aim of the current research was to search for osteogenic molecules affected by caspase inhibition and to specify the individual caspases critical for these effects with a focus on proapoptotic caspases: caspase-2, -3, -6, -7, -8 and -9. Along with osteocalcin (Ocn), general caspase inhibition significantly decreased the expression of the Phex gene in differentiated MC3T3-E1 cells. The inhibition of individual caspases indicated that caspase-8 is a major contributor to the modification of Ocn and Phex expression. Caspase-2 and-6 had effects on Ocn and caspase-6 had an effect on Phex. These data confirm and expand the current knowledge about the nonapoptotic roles of caspases and the effect of their pharmacological inhibition on the osteogenic potential of osteoblastic cells.

Návaznosti

GA19-14727S, projekt VaV

Název: Specifikace osteogenního potenciálu kaspáz v kontextu kraniofaciálního vývoje (Akronym: Kaspazy v kraniofacialnim vyvoji)

Investor: Grantová agentura ČR, Specifikace osteogenního potenciálu kaspáz v kontextu kraniofaciálního vývoje

Citovat

KRATOCHVÍLOVÁ, Adéla; Barbora VESELÁ; Vojtěch LEDVINA; Eva ŠVANDOVÁ; Karel KLEPÁRNÍK; Kateřina DADÁKOVÁ; Petr BENEŠ a Eva MATALOVÁ. Osteogenic impact of pro-apoptotic caspase inhibitors in MC3T3-E1 cells. Scientific Reports. London: Nature Publishing Group, 2020, roč. 10, č. 1, s. 1-8. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-020-64294-9.

@article{1668304,
   author = {Kratochvílová, Adéla and Veselá, Barbora and Ledvina, Vojtěch and Švandová, Eva and Klepárník, Karel and Dadáková, Kateřina and Beneš, Petr and Matalová, Eva},
   article_location = {London},
   article_number = {1},
   doi = {https://doi.org/10.1038/s41598-020-64294-9},
   keywords = {Bone; Bone development; Cell biology; Proteases},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Osteogenic impact of pro-apoptotic caspase inhibitors in MC3T3-E1 cells},
   url = {https://www.nature.com/articles/s41598-020-64294-9},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1668304
AU  - Kratochvílová, Adéla - Veselá, Barbora - Ledvina, Vojtěch - Švandová, Eva - Klepárník, Karel - Dadáková, Kateřina - Beneš, Petr - Matalová, Eva
PY  - 2020
TI  - Osteogenic impact of pro-apoptotic caspase inhibitors in MC3T3-E1 cells
JF  - Scientific Reports
VL  - 10
IS  - 1
SP  - 1-8
EP  - 1-8
PB  - Nature Publishing Group
SN  - 20452322
KW  - Bone
KW  - Bone development
KW  - Cell biology
KW  - Proteases
UR  - https://www.nature.com/articles/s41598-020-64294-9
L2  - https://www.nature.com/articles/s41598-020-64294-9
N2  - Caspases are proteases traditionally associated with inflammation and cell death. Recently, they have also been shown to modulate cell proliferation and differentiation. The aim of the current research was to search for osteogenic molecules affected by caspase inhibition and to specify the individual caspases critical for these effects with a focus on proapoptotic caspases: caspase-2, -3, -6, -7, -8 and -9. Along with osteocalcin (Ocn), general caspase inhibition significantly decreased the expression of the Phex gene in differentiated MC3T3-E1 cells. The inhibition of individual caspases indicated that caspase-8 is a major contributor to the modification of Ocn and Phex expression. Caspase-2 and-6 had effects on Ocn and caspase-6 had an effect on Phex. These data confirm and expand the current knowledge about the nonapoptotic roles of caspases and the effect of their pharmacological inhibition on the osteogenic potential of osteoblastic cells.
ER  -

KRATOCHVÍLOVÁ, Adéla; Barbora VESELÁ; Vojtěch LEDVINA; Eva ŠVANDOVÁ; Karel KLEPÁRNÍK; Kateřina DADÁKOVÁ; Petr BENEŠ a Eva MATALOVÁ. Osteogenic impact of pro-apoptotic caspase inhibitors in MC3T3-E1 cells. \textit{Scientific Reports}. London: Nature Publishing Group, 2020, roč.~10, č.~1, s.~1-8. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/s41598-020-64294-9.

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