CE study of neuroprotective humanin peptide and its
derivatives: Interactions with phosphate, sulphate,
alkylsulphonates and sulphated-beta-CD

J 2008

CE study of neuroprotective humanin peptide and its derivatives: Interactions with phosphate, sulphate, alkylsulphonates and sulphated-beta-CD

HAVEL, Josef; Rong LI a Mirek MACKA

Základní údaje

Originální název

CE study of neuroprotective humanin peptide and its derivatives: Interactions with phosphate, sulphate, alkylsulphonates and sulphated-beta-CD

Autoři

HAVEL, Josef; Rong LI a Mirek MACKA

Vydání

Electrophoresis, HOBOKEN, Verlag Chemie, 2008, 0173-0835

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.509

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

CE; humanin peptide derivatives; interaction; ion associates; sulphated-beta-CD

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 6. 2020 09:45, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Humanin (HN), Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-IIe-Asp-Leu- Pro-Val-Lys-Arg-Arg-Ala, recently discovered in the human brain, is an important neuroprotective peptide. Some derivatives of HN show even higher biological activity, for example [G-14]-HN, where Ser at position 14 is replaced with Gly. As structurally related HN peptide derivatives have similar chemical properties, their separation by CE is difficult. In this work, the electrophoretic behaviour of HN derivatives including [G-14]-HN, a tryptophan HN derivative [W-14]-HN, several other HN derivatives and HN fragments was studied. While phosphate buffer was used as the general BGE, the effects of the buffer concentration and various additives were examined, including sulphate, heptane sulphonate, 2-morpholinoethanesulphonic acid N-[tris(hydroxymethyl)methyl]-2-amino-ethane sulphonic acid (TES), sulphated-beta-CD and beta-CD. Separation efficiency of 200 000 theoretical plates was achieved in a BGE of 80 mM phosphate at pH 2.5 where seven out of nine major peaks were partially separated. By investigating the influence of concentration of the interrogated ions on peptides migration, the association between positively charged protonated sites of peptides and various anions was proved. Especially a strong interaction with phosphate, sulphate and sulphonate groups was established. Conditional stability constant of the [Pep(z+), (H2PO4-)(n)](z-n) ion associate (n = 1) for [G-14]-HN equals to log K similar to 1.78.

Návaznosti

GA305/03/1100, projekt VaV

Název: Syntéza a studium neuroprotektivních peptidů odvozených od humaninu

LC06035, projekt VaV

Název: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu

Citovat

HAVEL, Josef; Rong LI a Mirek MACKA. CE study of neuroprotective humanin peptide and its derivatives: Interactions with phosphate, sulphate, alkylsulphonates and sulphated-beta-CD. Electrophoresis. HOBOKEN: Verlag Chemie, 2008, roč. 29, č. 3, s. 665-671. ISSN 0173-0835. Dostupné z: https://doi.org/10.1002/elps.200700588.

@article{1668473,
   author = {Havel, Josef and Li, Rong and Macka, Mirek},
   article_location = {HOBOKEN},
   article_number = {3},
   doi = {https://doi.org/10.1002/elps.200700588},
   keywords = {CE; humanin peptide derivatives; interaction; ion associates; sulphated-beta-CD},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {CE study of neuroprotective humanin peptide and its derivatives: Interactions with phosphate, sulphate, alkylsulphonates and sulphated-beta-CD},
   url = {https://doi.org/10.1002/elps.200700588},
   volume = {29},
   year = {2008}
}

TY  - JOUR
ID  - 1668473
AU  - Havel, Josef - Li, Rong - Macka, Mirek
PY  - 2008
TI  - CE study of neuroprotective humanin peptide and its derivatives: Interactions with phosphate, sulphate, alkylsulphonates and sulphated-beta-CD
JF  - Electrophoresis
VL  - 29
IS  - 3
SP  - 665-671
EP  - 665-671
PB  - Verlag Chemie
SN  - 01730835
KW  - CE
KW  - humanin peptide derivatives
KW  - interaction
KW  - ion associates
KW  - sulphated-beta-CD
UR  - https://doi.org/10.1002/elps.200700588
L2  - https://doi.org/10.1002/elps.200700588
N2  - Humanin (HN), Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-IIe-Asp-Leu- Pro-Val-Lys-Arg-Arg-Ala, recently discovered in the human brain, is an important neuroprotective peptide. Some derivatives of HN show even higher biological activity, for example [G-14]-HN, where Ser at position 14 is replaced with Gly. As structurally related HN peptide derivatives have similar chemical properties, their separation by CE is difficult. In this work, the electrophoretic behaviour of HN derivatives including [G-14]-HN, a tryptophan HN derivative [W-14]-HN, several other HN derivatives and HN fragments was studied. While phosphate buffer was used as the general BGE, the effects of the buffer concentration and various additives were examined, including sulphate, heptane sulphonate, 2-morpholinoethanesulphonic acid N-[tris(hydroxymethyl)methyl]-2-amino-ethane sulphonic acid (TES), sulphated-beta-CD and beta-CD. Separation efficiency of 200 000 theoretical plates was achieved in a BGE of 80 mM phosphate at pH 2.5 where seven out of nine major peaks were partially separated. By investigating the influence of concentration of the interrogated ions on peptides migration, the association between positively charged protonated sites of peptides and various anions was proved. Especially a strong interaction with phosphate, sulphate and sulphonate groups was established. Conditional stability constant of the [Pep(z+), (H2PO4-)(n)](z-n) ion associate (n = 1) for [G-14]-HN equals to log K similar to 1.78.
ER  -

HAVEL, Josef; Rong LI a Mirek MACKA. CE study of neuroprotective humanin peptide and its derivatives: Interactions with phosphate, sulphate, alkylsulphonates and sulphated-beta-CD. \textit{Electrophoresis}. HOBOKEN: Verlag Chemie, 2008, roč.~29, č.~3, s.~665-671. ISSN~0173-0835. Dostupné z: https://doi.org/10.1002/elps.200700588.

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