Ancient DNA mining from chemically treated and non-treated
human dental calculus

a 2019

Ancient DNA mining from chemically treated and non-treated human dental calculus

NOVOTNÁ, Kateřina; Dana FIALOVÁ; Eva CHOCHOLOVÁ; Eva DROZDOVÁ; Kristýna BRZOBOHATÁ et al.

Základní údaje

Originální název

Ancient DNA mining from chemically treated and non-treated human dental calculus

Autoři

NOVOTNÁ, Kateřina; Dana FIALOVÁ; Eva CHOCHOLOVÁ ; Eva DROZDOVÁ; Kristýna BRZOBOHATÁ a Bohuslav KLÍMA

Vydání

6th International Anthropological Congress of Dr. Aleš Hrdlička, 2019

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00124839

Organizační jednotka

Přírodovědecká fakulta

ISSN

Klíčová slova anglicky

ancient dental calculus; aDNA; the Great Moravian Empire; chemical conservation; isolation of aDNA

Změněno: 1. 6. 2022 09:11, Mgr. Kateřina Novotná

Anotace

V originále

During the last century, scientists commonly underwent a procedure of preserving osteological material via chemical conservation. It was a standard routine, which continues to this day, especially when very rare finds, which are deposited in museums, are concerned. However, scientists assumed th at chemical conservation, which is supposed to protect the human remains from degradation, would actually prevent the aDNA from extraction and further analysis. Especially nowadays, when molecular methods are available and widely used in this research field. As a result of this procedure, many human remains are untouched by genetic analysis. This study focuses on the samples treated by chemical conservation, namely dental calculus of individuals from two burial sites dated back to the Great Moravian Empire (9th – 10th century AD). The first site Pohansko – Kostel, was excavated in the 1960s. The human remains have been treated by celluloid dissolved in amyl acetate and acetate solution. 409 graves were excavated, from which the individuals were selected based on the size and location of their dental calculus for purposes of our research. The other site is located in Znojmo – Hradiště. The excavation is recent and ongoing since 2007. These remains were never chemically treated, thus serving as a comparison for the isolation protocol and further analysis. The samples were selected based on the same parameters as those, which were applied to the samples from Pohansko – Kostel. The aDNA of dental calculus was isolated from both graveyards with MinEluteTM PCR purification Kit (Qiagen). The results were analysed thoroughly in order to clarify whether the conservation treatment enables further scientific research of such samples.

Návaznosti

MUNI/C/1703/2018, interní kód MU

Název: Analýza historického zubního kamene ošetřeného chemickou konzervací

Investor: Masarykova univerzita, Analýza historického zubního kamene ošetřeného chemickou konzervací, DO R. 2020 - Program rektora

Citovat

NOVOTNÁ, Kateřina; Dana FIALOVÁ; Eva CHOCHOLOVÁ; Eva DROZDOVÁ; Kristýna BRZOBOHATÁ a Bohuslav KLÍMA. Ancient DNA mining from chemically treated and non-treated human dental calculus. In 6th International Anthropological Congress of Dr. Aleš Hrdlička. 2019. ISSN 2336-4777.

@proceedings{1669918,
   author = {Novotná, Kateřina and Fialová, Dana and Chocholová, Eva and Drozdová, Eva and Brzobohatá, Kristýna and Klíma, Bohuslav},
   booktitle = {6th International Anthropological Congress of Dr. Aleš Hrdlička},
   keywords = {ancient dental calculus; aDNA; the Great Moravian Empire; chemical conservation; isolation of aDNA},
   language = {eng},
   title = {Ancient DNA mining from chemically treated and non-treated human dental calculus},
   url = {http://iacah.eu/static/abstrakta.pdf},
   year = {2019}
}

TY  - CONF
ID  - 1669918
AU  - Novotná, Kateřina - Fialová, Dana - Chocholová, Eva - Drozdová, Eva - Brzobohatá, Kristýna - Klíma, Bohuslav
PY  - 2019
TI  - Ancient DNA mining from chemically treated and non-treated human dental calculus
KW  - ancient dental calculus
KW  - aDNA
KW  - the Great Moravian Empire
KW  - chemical conservation
KW  - isolation of aDNA
UR  - http://iacah.eu/static/abstrakta.pdf
L2  - http://iacah.eu/static/abstrakta.pdf
N2  - During the last century, scientists commonly underwent a procedure of preserving osteological material via chemical conservation. It was a standard routine, which continues to this day, especially when very rare finds, which are deposited in museums, are concerned. However, scientists assumed th at chemical conservation, which is supposed to protect the human remains from degradation, would actually prevent the aDNA from extraction and further analysis. Especially nowadays, when molecular methods are available and widely used in this research field. As a result of this procedure, many human remains are untouched by genetic analysis. This study focuses on the samples treated by chemical conservation, namely dental calculus of individuals from two burial sites dated back to the Great Moravian Empire (9th – 10th century AD). The first site Pohansko – Kostel, was excavated in the 1960s. The human remains have been treated by celluloid dissolved in amyl acetate and acetate solution. 409 graves were excavated, from which the individuals were selected based on the size and location of their dental calculus for purposes of our research. The other site is located in Znojmo – Hradiště. The excavation is recent and ongoing since 2007. These remains were never chemically treated, thus serving as a comparison for the isolation protocol and further analysis. The samples were selected based on the same parameters as those, which were applied to the samples from Pohansko – Kostel. The aDNA of dental calculus was isolated from both graveyards with MinEluteTM PCR purification Kit (Qiagen). The results were analysed thoroughly in order to clarify whether the conservation treatment enables further scientific research of such samples.
ER  -

NOVOTNÁ, Kateřina; Dana FIALOVÁ; Eva CHOCHOLOVÁ; Eva DROZDOVÁ; Kristýna BRZOBOHATÁ a Bohuslav KLÍMA. Ancient DNA mining from chemically treated and non-treated human dental calculus. In \textit{6th International Anthropological Congress of Dr. Aleš Hrdlička}. 2019. ISSN~2336-4777.

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