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@article{1670353, author = {Germoglio, M. and Valenti, A. and Gallo, I. and Forenza, C. and Santonicola, P. and Silva, Nicola and Adamo, A.}, article_location = {LONDON}, article_number = {1}, doi = {http://dx.doi.org/10.1038/s41598-019-57096-1}, keywords = {DOUBLE-STRAND BREAK; FANCONI-ANEMIA PROTEINS; INTERSTRAND CROSS-LINKS; C-ELEGANS; REPAIR PATHWAY; RECOMBINATION; CHROMOSOME; MONOUBIQUITINATION; COMPLEX; CANCER}, language = {eng}, issn = {2045-2322}, journal = {Scientific reports}, title = {In vivo analysis of FANCD2 recruitment at meiotic DNA breaks in Caenorhabditis elegans}, url = {https://www.nature.com/articles/s41598-019-57096-1.pdf}, volume = {10}, year = {2020} }
TY - JOUR ID - 1670353 AU - Germoglio, M. - Valenti, A. - Gallo, I. - Forenza, C. - Santonicola, P. - Silva, Nicola - Adamo, A. PY - 2020 TI - In vivo analysis of FANCD2 recruitment at meiotic DNA breaks in Caenorhabditis elegans JF - Scientific reports VL - 10 IS - 1 SP - 1-14 EP - 1-14 PB - NATURE PUBLISHING GROUP SN - 20452322 KW - DOUBLE-STRAND BREAK KW - FANCONI-ANEMIA PROTEINS KW - INTERSTRAND CROSS-LINKS KW - C-ELEGANS KW - REPAIR PATHWAY KW - RECOMBINATION KW - CHROMOSOME KW - MONOUBIQUITINATION KW - COMPLEX KW - CANCER UR - https://www.nature.com/articles/s41598-019-57096-1.pdf L2 - https://www.nature.com/articles/s41598-019-57096-1.pdf N2 - Fanconi Anemia is a rare genetic disease associated with DNA repair defects, congenital abnormalities and infertility. Most of FA pathway is evolutionary conserved, allowing dissection and mechanistic studies in simpler model systems such as Caenorhabditis elegans. In the present study, we employed C. elegans to better understand the role of FA group D2 (FANCD2) protein in vivo, a key player in promoting genome stability. We report that localization of FCD-2/FANCD2 is dynamic during meiotic prophase I and requires its heterodimeric partner FNCI-1/FANCI. Strikingly, we found that FCD-2 recruitment depends on SPO-11-induced double-strand breaks (DSBs) but not RAD-51-mediated strand invasion. Furthermore, exposure to DNA damage-inducing agents boosts FCD-2 recruitment on the chromatin. Finally, analysis of genetic interaction between FCD-2 and BRC-1 (the C. elegans orthologue of mammalian BRCA1) supports a role for these proteins in different DSB repair pathways. Collectively, we showed a direct involvement of FCD-2 at DSBs and speculate on its function in driving meiotic DNA repair. ER -
GERMOGLIO, M., A. VALENTI, I. GALLO, C. FORENZA, P. SANTONICOLA, Nicola SILVA a A. ADAMO. In vivo analysis of FANCD2 recruitment at meiotic DNA breaks in Caenorhabditis elegans. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2020, roč.~10, č.~1, s.~1-14. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-019-57096-1.
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