The Beta Subunit of Nascent Polypeptide Associated Complex
Plays A Role in Flowers and Siliques Development of Arabidopsis
thaliana

J 2020

The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana

FÍLA, Jan, Božena KLODOVÁ, David POTĚŠIL, Miloslav JUŘÍČEK, Petr ŠESTÁK et. al.

Základní údaje

Originální název

The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana

Autoři

FÍLA, Jan (203 Česká republika), Božena KLODOVÁ (203 Česká republika), David POTĚŠIL (203 Česká republika, domácí), Miloslav JUŘÍČEK, Petr ŠESTÁK, Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí) a David HONYS

Vydání

International Journal of Molecular Sciences, Basel, MDPI, 2020, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 5.923

Kód RIV

RIV/00216224:14740/20:00114234

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/ijms21062065

UT WoS

000529890200161

Klíčová slova anglicky

Arabidopsis thaliana; chaperone; flower bud proteome; flower bud transcriptome; male gametophyte; nascent polypeptide-associated complex

Štítky

CF PROT, rivok

Změněno: 2. 11. 2024 20:33, Ing. Martina Blahová

Anotace

V originále

The nascent polypeptide-associated (NAC) complex was described in yeast as a heterodimer composed of two subunits, alpha and beta, and was shown to bind to the nascent polypeptides newly emerging from the ribosomes. NAC function was widely described in yeast and several information are also available about its role in plants. The knock down of individual NAC subunit(s) led usually to a higher sensitivity to stress. In Arabidopsis thaliana genome, there are five genes encoding NAC alpha subunit, and two genes encoding NAC beta. Double homozygous mutant in both genes coding for NAC beta was acquired, which showed a delayed development compared to the wild type, had abnormal number of flower organs, shorter siliques and greatly reduced seed set. Both NAC beta genes were characterized in more detail-the phenotype of the double homozygous mutant was complemented by a functional NAC beta copy. Then, both NAC beta genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nac beta mutation, the flower buds' transcriptome was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NAC beta subunits in stress responses, male gametophyte development, and photosynthesis.

Návaznosti

EF16_019/0000738, projekt VaV

Název: Centrum experimentální biologie rostlin

GA18-02448S, projekt VaV

Název: Úloha translačních iniciačních faktorů při skladování a aktivaci transkriptů v samčí zárodečné linii krytosemenných rostlin

Investor: Grantová agentura ČR, Úloha translačních iniciačních faktorů při skladování a aktivaci transkriptů v samčí zárodečné linii krytosemenných rostlin

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

FÍLA, Jan, Božena KLODOVÁ, David POTĚŠIL, Miloslav JUŘÍČEK, Petr ŠESTÁK, Zbyněk ZDRÁHAL a David HONYS. The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana. International Journal of Molecular Sciences. Basel: MDPI, 2020, roč. 21, č. 6, s. 1-30. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms21062065.

@article{1670471,
   author = {Fíla, Jan and Klodová, Božena and Potěšil, David and Juříček, Miloslav and Šesták, Petr and Zdráhal, Zbyněk and Honys, David},
   article_location = {Basel},
   article_number = {6},
   doi = {http://dx.doi.org/10.3390/ijms21062065},
   keywords = {Arabidopsis thaliana; chaperone; flower bud proteome; flower bud transcriptome; male gametophyte; nascent polypeptide-associated complex},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana},
   url = {https://www.mdpi.com/1422-0067/21/6/2065/htm},
   volume = {21},
   year = {2020}
}

TY  - JOUR
ID  - 1670471
AU  - Fíla, Jan - Klodová, Božena - Potěšil, David - Juříček, Miloslav - Šesták, Petr - Zdráhal, Zbyněk - Honys, David
PY  - 2020
TI  - The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana
JF  - International Journal of Molecular Sciences
VL  - 21
IS  - 6
SP  - 1-30
EP  - 1-30
PB  - MDPI
SN  - 14220067
KW  - Arabidopsis thaliana
KW  - chaperone
KW  - flower bud proteome
KW  - flower bud transcriptome
KW  - male gametophyte
KW  - nascent polypeptide-associated complex
UR  - https://www.mdpi.com/1422-0067/21/6/2065/htm
L2  - https://www.mdpi.com/1422-0067/21/6/2065/htm
N2  - The nascent polypeptide-associated (NAC) complex was described in yeast as a heterodimer composed of two subunits, alpha and beta, and was shown to bind to the nascent polypeptides newly emerging from the ribosomes. NAC function was widely described in yeast and several information are also available about its role in plants. The knock down of individual NAC subunit(s) led usually to a higher sensitivity to stress. In Arabidopsis thaliana genome, there are five genes encoding NAC alpha subunit, and two genes encoding NAC beta. Double homozygous mutant in both genes coding for NAC beta was acquired, which showed a delayed development compared to the wild type, had abnormal number of flower organs, shorter siliques and greatly reduced seed set. Both NAC beta genes were characterized in more detail-the phenotype of the double homozygous mutant was complemented by a functional NAC beta copy. Then, both NAC beta genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nac beta mutation, the flower buds' transcriptome was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NAC beta subunits in stress responses, male gametophyte development, and photosynthesis.
ER  -

FÍLA, Jan, Božena KLODOVÁ, David POTĚŠIL, Miloslav JUŘÍČEK, Petr ŠESTÁK, Zbyněk ZDRÁHAL a David HONYS. The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2020, roč.~21, č.~6, s.~1-30. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms21062065.

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