Selenium Incorporation to Amino Acids in Chlorella Cultures
Grown in Phototrophic and Heterotrophic Regimes

J 2020

Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes

MYLENKO, Mykola; Dai Long VU; Jan KUTA; Karolina RANGLOVA; David KUBÁČ et al.

Základní údaje

Originální název

Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes

Autoři

Vydání

Journal of Agricultural and Food Chemistry, Washington, D.C. American Chemical Society, 2020, 0021-8561

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

21101 Food and beverages

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.279

Kód RIV

RIV/00216224:14310/20:00116084

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1021/acs.jafc.9b06196

UT WoS

000514256200016

EID Scopus

2-s2.0-85079331947

Klíčová slova anglicky

Chlorella; microalgae; selenium; selenomethionine; selenocysteine; methylselenocysteine; phototrophic cultivation; heterotrophic cultivation

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 6. 2025 20:41, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Microalgae accumulate bioavailable selenium-containing amino acids (Se-AAs), and these are useful as a food supplement. While this accumulation has been studied in phototrophic algal cultures, little data exists for heterotrophic cultures. We have determined the Se-AAs content, selenium/sulfur (Se/S) substitution rates, and overall Se accumulation balance in photo-and heterotrophic Chlorella cultures. Laboratory trials revealed that heterotrophic cultures tolerate Se doses, similar to 8-fold higher compared to phototrophic cultures, resulting in a similar to 2-3-fold higher Se-AAs content. In large-scale experiments, both cultivation regimes provided comparable Se-AAs content. Outdoor phototrophic cultures accumulated up to 400 mu g g(-1) of total Se-AAs and exhibited a high level of Se/S substitution (5-10%) with 30-60% organic/total Se embedded in the biomass. A slightly higher content of Se-AAs and ratio of Se/S substitution was obtained for a heterotrophic culture in pilot-scale fermentors. The data presented here shows that heterotrophic Chlorella cultures provide an alternative for Se-enriched biomass production and provides information on Se-AAs content and speciation in different cultivation regimes.

Návaznosti

EF16_013/0001761, projekt VaV

Název: RECETOX RI

90051, velká výzkumná infrastruktura

Název: RECETOX II

Citovat

MYLENKO, Mykola; Dai Long VU; Jan KUTA; Karolina RANGLOVA; David KUBÁČ; Gergely LAKATOS; Tomas GRIVALSKY; Martin Pablo CAPORGNO; Joao Artur Camara MANOEL; Jiri KOPECKY; Jiří MASOJÍDEK a Pavel HROUZEK. Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes. Journal of Agricultural and Food Chemistry. Washington, D.C.: American Chemical Society, 2020, roč. 68, č. 6, s. 1654-1665. ISSN 0021-8561. Dostupné z: https://doi.org/10.1021/acs.jafc.9b06196.

@article{1672104,
   author = {Mylenko, Mykola and Vu, Dai Long and Kuta, Jan and Ranglova, Karolina and Kubáč, David and Lakatos, Gergely and Grivalsky, Tomas and Caporgno, Martin Pablo and Manoel, Joao Artur Camara and Kopecky, Jiri and Masojídek, Jiří and Hrouzek, Pavel},
   article_location = {Washington, D.C.},
   article_number = {6},
   doi = {https://doi.org/10.1021/acs.jafc.9b06196},
   keywords = {Chlorella; microalgae; selenium; selenomethionine; selenocysteine; methylselenocysteine; phototrophic cultivation; heterotrophic cultivation},
   language = {eng},
   issn = {0021-8561},
   journal = {Journal of Agricultural and Food Chemistry},
   title = {Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes},
   url = {https://pubs.acs.org/doi/10.1021/acs.jafc.9b06196},
   volume = {68},
   year = {2020}
}

TY  - JOUR
ID  - 1672104
AU  - Mylenko, Mykola - Vu, Dai Long - Kuta, Jan - Ranglova, Karolina - Kubáč, David - Lakatos, Gergely - Grivalsky, Tomas - Caporgno, Martin Pablo - Manoel, Joao Artur Camara - Kopecky, Jiri - Masojídek, Jiří - Hrouzek, Pavel
PY  - 2020
TI  - Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes
JF  - Journal of Agricultural and Food Chemistry
VL  - 68
IS  - 6
SP  - 1654-1665
EP  - 1654-1665
PB  - American Chemical Society
SN  - 00218561
KW  - Chlorella
KW  - microalgae
KW  - selenium
KW  - selenomethionine
KW  - selenocysteine
KW  - methylselenocysteine
KW  - phototrophic cultivation
KW  - heterotrophic cultivation
UR  - https://pubs.acs.org/doi/10.1021/acs.jafc.9b06196
L2  - https://pubs.acs.org/doi/10.1021/acs.jafc.9b06196
N2  - Microalgae accumulate bioavailable selenium-containing amino acids (Se-AAs), and these are useful as a food supplement. While this accumulation has been studied in phototrophic algal cultures, little data exists for heterotrophic cultures. We have determined the Se-AAs content, selenium/sulfur (Se/S) substitution rates, and overall Se accumulation balance in photo-and heterotrophic Chlorella cultures. Laboratory trials revealed that heterotrophic cultures tolerate Se doses, similar to 8-fold higher compared to phototrophic cultures, resulting in a similar to 2-3-fold higher Se-AAs content. In large-scale experiments, both cultivation regimes provided comparable Se-AAs content. Outdoor phototrophic cultures accumulated up to 400 mu g g(-1) of total Se-AAs and exhibited a high level of Se/S substitution (5-10%) with 30-60% organic/total Se embedded in the biomass. A slightly higher content of Se-AAs and ratio of Se/S substitution was obtained for a heterotrophic culture in pilot-scale fermentors. The data presented here shows that heterotrophic Chlorella cultures provide an alternative for Se-enriched biomass production and provides information on Se-AAs content and speciation in different cultivation regimes.
ER  -

MYLENKO, Mykola; Dai Long VU; Jan KUTA; Karolina RANGLOVA; David KUBÁČ; Gergely LAKATOS; Tomas GRIVALSKY; Martin Pablo CAPORGNO; Joao Artur Camara MANOEL; Jiri KOPECKY; Jiří MASOJÍDEK a Pavel HROUZEK. Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes. \textit{Journal of Agricultural and Food Chemistry}. Washington, D.C.: American Chemical Society, 2020, roč.~68, č.~6, s.~1654-1665. ISSN~0021-8561. Dostupné z: https://doi.org/10.1021/acs.jafc.9b06196.

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