Microcystin-LR Does Not Alter Cell Survival and Intracellular
Signaling in Human Bronchial Epithelial Cells

J 2020

Microcystin-LR Does Not Alter Cell Survival and Intracellular Signaling in Human Bronchial Epithelial Cells

BRÓZMAN, Ondřej; Barbara KUBÍČKOVÁ; Pavel BABICA a Petra LABOHÁ

Základní údaje

Originální název

Microcystin-LR Does Not Alter Cell Survival and Intracellular Signaling in Human Bronchial Epithelial Cells

Autoři

BRÓZMAN, Ondřej; Barbara KUBÍČKOVÁ; Pavel BABICA a Petra LABOHÁ

Vydání

Toxins, Basel, MDPI, 2020, 2072-6651

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

21101 Food and beverages

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.546

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/20:00114249

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

microcystin-LR; human bronchial epithelial cells; in vitro; HBE1; 16HBE14o-; mitogen-activated protein kinase; cytotoxicity; OATP

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 6. 2025 20:35, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Changes in ecological and environmental factors lead to an increased occurrence of cyanobacterial water blooms, while secondary metabolites-producing cyanobacteria pose a threat to both environmental and human health. Apart from oral and dermal exposure, humans may be exposed via inhalation and/or swallowing of contaminated water and aerosols. Although many studies deal with liver toxicity, less information about the effects in the respiratory system is available. We investigated the effects of a prevalent cyanotoxin, microcystin-LR (MC-LR), using respiratory system-relevant human bronchial epithelial (HBE) cells. The expression of specific organic-anion-transporting polypeptides was evaluated, and the western blot analysis revealed the formation and accumulation of MC-LR protein adducts in exposed cells. However, MC-LR up to 20 mu M neither caused significant cytotoxic effects according to multiple viability endpoints after 48-h exposure, nor reduced impedance (cell layer integrity) over 96 h. Time-dependent increase of putative MC-LR adducts with protein phosphatases was not associated with activation of mitogen-activated protein kinases ERK1/2 and p38 during 48-h exposure in HBE cells. Future studies addressing human health risks associated with inhalation of toxic cyanobacteria and cyanotoxins should focus on complex environmental samples of cyanobacterial blooms and alterations of additional non-cytotoxic endpoints while adopting more advanced in vitro models.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

GJ17-25279Y, projekt VaV

Název: Zdravotní rizika spojená s inhalací sinic

Investor: Grantová agentura ČR, Zdravotní rizika spojená s inhalací sinic

722493, interní kód MU

Název: Natural Toxins and Drinking Water Quality - From Source to Tap (Akronym: NaToxAq)

Investor: Evropská unie, Natural Toxins and Drinking Water Quality - From Source to Tap, MSCA Marie Skłodowska-Curie Actions (Excellent Science)

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

90121, velká výzkumná infrastruktura

Název: RECETOX RI

Přiložené soubory

1672261.pdf

Citovat

BRÓZMAN, Ondřej; Barbara KUBÍČKOVÁ; Pavel BABICA a Petra LABOHÁ. Microcystin-LR Does Not Alter Cell Survival and Intracellular Signaling in Human Bronchial Epithelial Cells. Toxins. Basel: MDPI, 2020, roč. 12, č. 3, s. 1-19. ISSN 2072-6651. Dostupné z: https://doi.org/10.3390/toxins12030165.

@article{1672261,
   author = {Brózman, Ondřej and Kubíčková, Barbara and Babica, Pavel and Labohá, Petra},
   article_location = {Basel},
   article_number = {3},
   doi = {https://doi.org/10.3390/toxins12030165},
   keywords = {microcystin-LR; human bronchial epithelial cells; in vitro; HBE1; 16HBE14o-; mitogen-activated protein kinase; cytotoxicity; OATP},
   language = {eng},
   issn = {2072-6651},
   journal = {Toxins},
   note = {CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat.},
   title = {Microcystin-LR Does Not Alter Cell Survival and Intracellular Signaling in Human Bronchial Epithelial Cells},
   url = {https://www.mdpi.com/2072-6651/12/3/165},
   volume = {12},
   year = {2020}
}

TY  - JOUR
ID  - 1672261
AU  - Brózman, Ondřej - Kubíčková, Barbara - Babica, Pavel - Labohá, Petra
PY  - 2020
TI  - Microcystin-LR Does Not Alter Cell Survival and Intracellular Signaling in Human Bronchial Epithelial Cells
JF  - Toxins
VL  - 12
IS  - 3
SP  - 1-19
EP  - 1-19
PB  - MDPI
SN  - 20726651
N1  - CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat.
KW  - microcystin-LR
KW  - human bronchial epithelial cells
KW  - in vitro
KW  - HBE1
KW  - 16HBE14o-
KW  - mitogen-activated protein kinase
KW  - cytotoxicity
KW  - OATP
UR  - https://www.mdpi.com/2072-6651/12/3/165
L2  - https://www.mdpi.com/2072-6651/12/3/165
N2  - Changes in ecological and environmental factors lead to an increased occurrence of cyanobacterial water blooms, while secondary metabolites-producing cyanobacteria pose a threat to both environmental and human health. Apart from oral and dermal exposure, humans may be exposed via inhalation and/or swallowing of contaminated water and aerosols. Although many studies deal with liver toxicity, less information about the effects in the respiratory system is available. We investigated the effects of a prevalent cyanotoxin, microcystin-LR (MC-LR), using respiratory system-relevant human bronchial epithelial (HBE) cells. The expression of specific organic-anion-transporting polypeptides was evaluated, and the western blot analysis revealed the formation and accumulation of MC-LR protein adducts in exposed cells. However, MC-LR up to 20 mu M neither caused significant cytotoxic effects according to multiple viability endpoints after 48-h exposure, nor reduced impedance (cell layer integrity) over 96 h. Time-dependent increase of putative MC-LR adducts with protein phosphatases was not associated with activation of mitogen-activated protein kinases ERK1/2 and p38 during 48-h exposure in HBE cells. Future studies addressing human health risks associated with inhalation of toxic cyanobacteria and cyanotoxins should focus on complex environmental samples of cyanobacterial blooms and alterations of additional non-cytotoxic endpoints while adopting more advanced in vitro models.
ER  -

BRÓZMAN, Ondřej; Barbara KUBÍČKOVÁ; Pavel BABICA a Petra LABOHÁ. Microcystin-LR Does Not Alter Cell Survival and Intracellular Signaling in Human Bronchial Epithelial Cells. \textit{Toxins}. Basel: MDPI, 2020, roč.~12, č.~3, s.~1-19. ISSN~2072-6651. Dostupné z: https://doi.org/10.3390/toxins12030165.

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