2020
Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes of Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
ROUDNICKÝ, Pavel, David POTĚŠIL, Zbyněk ZDRÁHAL, Milan GELNAR, Martin KAŠNÝ et. al.Základní údaje
Originální název
Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes of Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
Autoři
ROUDNICKÝ, Pavel (203 Česká republika, garant, domácí), David POTĚŠIL (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, domácí), Milan GELNAR (203 Česká republika, domácí) a Martin KAŠNÝ (203 Česká republika, domácí)
Vydání
PLOS ONE, San Francisco, Public Library of Science, 2020, 1932-6203
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10613 Zoology
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 3.240
Kód RIV
RIV/00216224:14310/20:00114261
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000542759600012
Klíčová slova anglicky
helminths; parasite; peptidase; inhibitor; laser microdissection; mass spectrometry
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 2. 11. 2024 20:30, Ing. Martina Blahová
Anotace
V originále
Eudiplozoon nipponicum (Goto, 1891) is a hematophagous monogenean ectoparasite which inhabits the gills of the common carp (Cyprinus carpio). Heavy infestation can lead to anemia and in conjunction with secondary bacterial infections cause poor health and eventual death of the host. This study is based on an innovative approach to protein localization which has never been used in parasitology before. Using laser capture microdissection, we dissected particular areas of the parasite body without contaminating the samples by surrounding tissue and in combination with analysis by mass spectrometry obtained tissue-specific proteomes of tegument, intestine, and parenchyma of our model organism, E. nipponicum. We successfully verified the presence of certain functional proteins (e.g. cathepsin L) in tissues where their presence was expected (intestine) and confirmed that there were no traces of these proteins in other tissues (tegument and parenchyma). Additionally, we identified a total of 2,059 proteins, including 72 peptidases and 33 peptidase inhibitors. As expected, the greatest variety was found in the intestine and the lowest variety in the parenchyma. Our results are significant on two levels. Firstly, we demonstrated that one can localize all proteins in one analysis and without using laboratory animals (antibodies for immunolocalization of single proteins). Secondly, this study offers the first complex proteomic data on not only the E. nipponicum but within the whole class of Monogenea, which was from this point of view until recently neglected.
Návaznosti
| GAP506/12/1258, projekt VaV |
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| GBP505/12/G112, projekt VaV |
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| LM2018140, projekt VaV |
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| LQ1601, projekt VaV |
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| MUNI/A/0918/2018, interní kód MU |
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| 90127, velká výzkumná infrastruktura |
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| 90132, velká výzkumná infrastruktura |
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