Functionalizable Antifouling Coatings as Tunable Platforms for
the Stress-Driven Manipulation of Living Cell Machinery

J 2020

Functionalizable Antifouling Coatings as Tunable Platforms for the Stress-Driven Manipulation of Living Cell Machinery

VÍŠOVÁ, Ivana, Barbora SMOLKOVÁ, Mariia UZHYTCHAK, Markéta VRABCOVÁ, Djamel Eddine CHAFAI et. al.

Basic information

Original name

Functionalizable Antifouling Coatings as Tunable Platforms for the Stress-Driven Manipulation of Living Cell Machinery

Authors

VÍŠOVÁ, Ivana (203 Czech Republic), Barbora SMOLKOVÁ (203 Czech Republic), Mariia UZHYTCHAK (203 Czech Republic), Markéta VRABCOVÁ (203 Czech Republic), Djamel Eddine CHAFAI (203 Czech Republic), Milan HOUSKA (203 Czech Republic), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution), Alexandr DEJNEKA (203 Czech Republic) and Hana VAISOCHEROVÁ-LÍSALOVÁ (203 Czech Republic)

Edition

Biomolecules, Basel, MDPI, 2020, 2218-273X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10404 Polymer science

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.879

RIV identification code

RIV/00216224:14310/20:00116149

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/biom10081146

UT WoS

000577776900001

Keywords in English

zwitterionic material; cell mechanotransduction; cell signaling; functional biointerfaces; antifouling polymer brushes; surface modification

Abstract

V originále

Cells are continuously sensing their microenvironment and subsequently respond to different physicochemical cues by the activation or inhibition of different signaling pathways. To study a very complex cellular response, it is necessary to diminish background environmental influences and highlight the particular event. However, surface-driven nonspecific interactions of the abundant biomolecules from the environment influence the targeted cell response significantly. Yes-associated protein (YAP) translocation may serve as a marker of human hepatocellular carcinoma (Huh7) cell responses to the extracellular matrix and surface-mediated stresses. Here, we propose a platform of tunable functionable antifouling poly(carboxybetain) (pCB)-based brushes to achieve a molecularly clean background for studying arginine, glycine, and aspartic acid (RGD)-induced YAP-connected mechanotransduction. Using two different sets of RGD-functionalized zwitterionic antifouling coatings with varying compositions of the antifouling layer, a clear correlation of YAP distribution with RGD functionalization concentrations was observed. On the other hand, commonly used surface passivation by the oligo(ethylene glycol)-based self-assembled monolayer (SAM) shows no potential to induce dependency of the YAP distribution on RGD concentrations. The results indicate that the antifouling background is a crucial component of surface-based cellular response studies, and pCB-based zwitterionic antifouling brush architectures may serve as a potential next-generation easily functionable surface platform for the monitoring and quantification of cellular processes.

Links

MUNI/A/1252/2019, interní kód MU

Name: Podpora biochemického výzkumu v roce 2020

Investor: Masaryk University, Category A

Citovat

VÍŠOVÁ, Ivana, Barbora SMOLKOVÁ, Mariia UZHYTCHAK, Markéta VRABCOVÁ, Djamel Eddine CHAFAI, Milan HOUSKA, Matěj PASTUCHA, Petr SKLÁDAL, Zdeněk FARKA, Alexandr DEJNEKA and Hana VAISOCHEROVÁ-LÍSALOVÁ. Functionalizable Antifouling Coatings as Tunable Platforms for the Stress-Driven Manipulation of Living Cell Machinery. Biomolecules. Basel: MDPI, 2020, vol. 10, No 8, p. 1-14. ISSN 2218-273X. Available from: https://dx.doi.org/10.3390/biom10081146.

@article{1673419,
   author = {Víšová, Ivana and Smolková, Barbora and Uzhytchak, Mariia and Vrabcová, Markéta and Chafai, Djamel Eddine and Houska, Milan and Pastucha, Matěj and Skládal, Petr and Farka, Zdeněk and Dejneka, Alexandr and VaisocherováandLísalová, Hana},
   article_location = {Basel},
   article_number = {8},
   doi = {http://dx.doi.org/10.3390/biom10081146},
   keywords = {zwitterionic material; cell mechanotransduction; cell signaling; functional biointerfaces; antifouling polymer brushes; surface modification},
   language = {eng},
   issn = {2218-273X},
   journal = {Biomolecules},
   title = {Functionalizable Antifouling Coatings as Tunable Platforms for the Stress-Driven Manipulation of Living Cell Machinery},
   url = {https://www.mdpi.com/2218-273X/10/8/1146},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1673419
AU  - Víšová, Ivana - Smolková, Barbora - Uzhytchak, Mariia - Vrabcová, Markéta - Chafai, Djamel Eddine - Houska, Milan - Pastucha, Matěj - Skládal, Petr - Farka, Zdeněk - Dejneka, Alexandr - Vaisocherová-Lísalová, Hana
PY  - 2020
TI  - Functionalizable Antifouling Coatings as Tunable Platforms for the Stress-Driven Manipulation of Living Cell Machinery
JF  - Biomolecules
VL  - 10
IS  - 8
SP  - 1-14
EP  - 1-14
PB  - MDPI
SN  - 2218273X
KW  - zwitterionic material
KW  - cell mechanotransduction
KW  - cell signaling
KW  - functional biointerfaces
KW  - antifouling polymer brushes
KW  - surface modification
UR  - https://www.mdpi.com/2218-273X/10/8/1146
L2  - https://www.mdpi.com/2218-273X/10/8/1146
N2  - Cells are continuously sensing their microenvironment and subsequently respond to different physicochemical cues by the activation or inhibition of different signaling pathways. To study a very complex cellular response, it is necessary to diminish background environmental influences and highlight the particular event. However, surface-driven nonspecific interactions of the abundant biomolecules from the environment influence the targeted cell response significantly. Yes-associated protein (YAP) translocation may serve as a marker of human hepatocellular carcinoma (Huh7) cell responses to the extracellular matrix and surface-mediated stresses. Here, we propose a platform of tunable functionable antifouling poly(carboxybetain) (pCB)-based brushes to achieve a molecularly clean background for studying arginine, glycine, and aspartic acid (RGD)-induced YAP-connected mechanotransduction. Using two different sets of RGD-functionalized zwitterionic antifouling coatings with varying compositions of the antifouling layer, a clear correlation of YAP distribution with RGD functionalization concentrations was observed. On the other hand, commonly used surface passivation by the oligo(ethylene glycol)-based self-assembled monolayer (SAM) shows no potential to induce dependency of the YAP distribution on RGD concentrations. The results indicate that the antifouling background is a crucial component of surface-based cellular response studies, and pCB-based zwitterionic antifouling brush architectures may serve as a potential next-generation easily functionable surface platform for the monitoring and quantification of cellular processes.
ER  -

VÍŠOVÁ, Ivana, Barbora SMOLKOVÁ, Mariia UZHYTCHAK, Markéta VRABCOVÁ, Djamel Eddine CHAFAI, Milan HOUSKA, Matěj PASTUCHA, Petr SKLÁDAL, Zdeněk FARKA, Alexandr DEJNEKA and Hana VAISOCHEROVÁ-LÍSALOVÁ. Functionalizable Antifouling Coatings as Tunable Platforms for the Stress-Driven Manipulation of Living Cell Machinery. \textit{Biomolecules}. Basel: MDPI, 2020, vol.~10, No~8, p.~1-14. ISSN~2218-273X. Available from: https://dx.doi.org/10.3390/biom10081146.

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