Phospholipid profiling enables to discriminate tumor- and
non-tumor-derived human colon epithelial cells: Phospholipidome
similarities and differences in colon cancer cell lines and in
patient-derived cell samples

J 2020

Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples

HOFMANOVA, Jirina, Josef SLAVIK, Petra OVESNÁ, Zuzana TYLICHOVA, Ladislav DUŠEK et. al.

Základní údaje

Originální název

Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples

Autoři

HOFMANOVA, Jirina (203 Česká republika), Josef SLAVIK (203 Česká republika), Petra OVESNÁ (203 Česká republika, domácí), Zuzana TYLICHOVA (203 Česká republika), Ladislav DUŠEK (203 Česká republika, domácí), Nicol STRAKOVA (203 Česká republika), Alena VACULOVA HYRSLOVA (203 Česká republika), Miroslav CIGANEK (203 Česká republika), Zdeněk KALA (203 Česká republika), Miroslav JÍRA (203 Česká republika), Igor PENKA (203 Česká republika), Jitka KYCLOVÁ (203 Česká republika), Zdenek KOLAR (203 Česká republika), Alois KOZUBÍK (203 Česká republika, domácí), Miroslav MACHALA (203 Česká republika) a Jan VONDRACEK (203 Česká republika, garant)

Vydání

Plos one, San Francisco, Public Library of Science, 2020, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.240

Kód RIV

RIV/00216224:14110/20:00116239

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0228010

UT WoS

000534612400041

Klíčová slova anglicky

HUMAN COLORECTAL-CANCER; FATTY-ACID; ADENOMA; PHOSPHATIDYLCHOLINE; PROTEIN; DIFFERENTIATION; ADENOCARCINOMA; PROLIFERATION; ABNORMALITIES; INVOLVEMENT

Štítky

14119612, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 5. 2021 07:59, Mgr. Tereza Miškechová

Anotace

V originále

Identification of changes of phospholipid (PL) composition occurring during colorectal cancer (CRC) development may help us to better understand their roles in CRC cells. Here, we used LC-MS/MS-based PL profiling of cell lines derived from normal colon mucosa, or isolated at distinct stages of CRC development, in order to study alterations of PL species potentially linked with cell transformation. We found that a detailed evaluation of phosphatidylinositol (PI) and phosphatidylserine (PS) classes allowed us to cluster the studied epithelial cell lines according to their origin: i) cells originally derived from normal colon tissue (NCM460, FHC); ii) cell lines derived from colon adenoma or less advanced differentiating adenocarcinoma cells (AA/C1, HT-29); or, iii) cells obtained by in vitro transformation of adenoma cells and advanced colon adenocarcinoma cells (HCT-116, AA/C1/SB10, SW480, SW620). Although we tentatively identified several PS and PI species contributing to cell line clustering, full PI and PS profiles appeared to be a key to the successful cell line discrimination. In parallel, we compared PL composition of primary epithelial (EpCAM-positive) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients, with PL profiles of cell lines derived from normal colon mucosa (NCM460) and from colon adenocarcinoma (HCT-116, SW480) cells, respectively. In general, higher total levels of all PL classes were observed in tumor cells. The overall PL profiles of the cell lines, when compared with the respective patient-derived cells, exhibited similarities. Nevertheless, there were also some notable differences in levels of individual PL species. This indicated that epithelial cell lines, derived either from normal colon tissue or from CRC cells, could be employed as models for functional lipidomic analyses of colon cells, albeit with some caution. The biological significance of the observed PL deregulation, or their potential links with specific CRC stages, deserve further investigation.

Citovat

HOFMANOVA, Jirina, Josef SLAVIK, Petra OVESNÁ, Zuzana TYLICHOVA, Ladislav DUŠEK, Nicol STRAKOVA, Alena VACULOVA HYRSLOVA, Miroslav CIGANEK, Zdeněk KALA, Miroslav JÍRA, Igor PENKA, Jitka KYCLOVÁ, Zdenek KOLAR, Alois KOZUBÍK, Miroslav MACHALA a Jan VONDRACEK. Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples. Plos one. San Francisco: Public Library of Science, 2020, roč. 15, č. 1, s. 1-21. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0228010.

@article{1674766,
   author = {Hofmanova, Jirina and Slavik, Josef and Ovesná, Petra and Tylichova, Zuzana and Dušek, Ladislav and Strakova, Nicol and Vaculova Hyrslova, Alena and Ciganek, Miroslav and Kala, Zdeněk and Jíra, Miroslav and Penka, Igor and Kyclová, Jitka and Kolar, Zdenek and Kozubík, Alois and Machala, Miroslav and Vondracek, Jan},
   article_location = {San Francisco},
   article_number = {1},
   doi = {http://dx.doi.org/10.1371/journal.pone.0228010},
   keywords = {HUMAN COLORECTAL-CANCER; FATTY-ACID; ADENOMA; PHOSPHATIDYLCHOLINE; PROTEIN; DIFFERENTIATION; ADENOCARCINOMA; PROLIFERATION; ABNORMALITIES; INVOLVEMENT},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples},
   url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228010},
   volume = {15},
   year = {2020}
}

TY  - JOUR
ID  - 1674766
AU  - Hofmanova, Jirina - Slavik, Josef - Ovesná, Petra - Tylichova, Zuzana - Dušek, Ladislav - Strakova, Nicol - Vaculova Hyrslova, Alena - Ciganek, Miroslav - Kala, Zdeněk - Jíra, Miroslav - Penka, Igor - Kyclová, Jitka - Kolar, Zdenek - Kozubík, Alois - Machala, Miroslav - Vondracek, Jan
PY  - 2020
TI  - Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples
JF  - Plos one
VL  - 15
IS  - 1
SP  - 1-21
EP  - 1-21
PB  - Public Library of Science
SN  - 19326203
KW  - HUMAN COLORECTAL-CANCER
KW  - FATTY-ACID
KW  - ADENOMA
KW  - PHOSPHATIDYLCHOLINE
KW  - PROTEIN
KW  - DIFFERENTIATION
KW  - ADENOCARCINOMA
KW  - PROLIFERATION
KW  - ABNORMALITIES
KW  - INVOLVEMENT
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228010
L2  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228010
N2  - Identification of changes of phospholipid (PL) composition occurring during colorectal cancer (CRC) development may help us to better understand their roles in CRC cells. Here, we used LC-MS/MS-based PL profiling of cell lines derived from normal colon mucosa, or isolated at distinct stages of CRC development, in order to study alterations of PL species potentially linked with cell transformation. We found that a detailed evaluation of phosphatidylinositol (PI) and phosphatidylserine (PS) classes allowed us to cluster the studied epithelial cell lines according to their origin: i) cells originally derived from normal colon tissue (NCM460, FHC); ii) cell lines derived from colon adenoma or less advanced differentiating adenocarcinoma cells (AA/C1, HT-29); or, iii) cells obtained by in vitro transformation of adenoma cells and advanced colon adenocarcinoma cells (HCT-116, AA/C1/SB10, SW480, SW620). Although we tentatively identified several PS and PI species contributing to cell line clustering, full PI and PS profiles appeared to be a key to the successful cell line discrimination. In parallel, we compared PL composition of primary epithelial (EpCAM-positive) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients, with PL profiles of cell lines derived from normal colon mucosa (NCM460) and from colon adenocarcinoma (HCT-116, SW480) cells, respectively. In general, higher total levels of all PL classes were observed in tumor cells. The overall PL profiles of the cell lines, when compared with the respective patient-derived cells, exhibited similarities. Nevertheless, there were also some notable differences in levels of individual PL species. This indicated that epithelial cell lines, derived either from normal colon tissue or from CRC cells, could be employed as models for functional lipidomic analyses of colon cells, albeit with some caution. The biological significance of the observed PL deregulation, or their potential links with specific CRC stages, deserve further investigation.
ER  -

HOFMANOVA, Jirina, Josef SLAVIK, Petra OVESNÁ, Zuzana TYLICHOVA, Ladislav DUŠEK, Nicol STRAKOVA, Alena VACULOVA HYRSLOVA, Miroslav CIGANEK, Zdeněk KALA, Miroslav JÍRA, Igor PENKA, Jitka KYCLOVÁ, Zdenek KOLAR, Alois KOZUBÍK, Miroslav MACHALA a Jan VONDRACEK. Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples. \textit{Plos one}. San Francisco: Public Library of Science, 2020, roč.~15, č.~1, s.~1-21. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0228010.

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