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@inproceedings{1675552,
author = {Dostalova, S. and Konecna, R. and Blazkova, I. and Vaculovicova, M. and Kopel, P. and Krizkova, S. and Vaculovič, Tomáš and Adam, V. and Kizek, R.},
address = {BRNO},
booktitle = {MENDELNET 2013},
editor = {Skarpa, P Ryant, P Cerkal, R Polak, O Kovarnik, J},
keywords = {apoferritin; doxorubicin; theranostics; antibodies; targeted delivery},
howpublished = {elektronická verze "online"},
language = {eng},
location = {BRNO},
isbn = {978-80-7375-908-7},
pages = {908-912},
publisher = {MENDEL UNIV BRNO, FAC AGRONOMY},
title = {APOFERRITIN AS A TARGETED DRUG DELIVERY SYSTEM},
year = {2013}
}
TY - JOUR
ID - 1675552
AU - Dostalova, S. - Konecna, R. - Blazkova, I. - Vaculovicova, M. - Kopel, P. - Krizkova, S. - Vaculovič, Tomáš - Adam, V. - Kizek, R.
PY - 2013
TI - APOFERRITIN AS A TARGETED DRUG DELIVERY SYSTEM
PB - MENDEL UNIV BRNO, FAC AGRONOMY
CY - BRNO
SN - 9788073759087
KW - apoferritin
KW - doxorubicin
KW - theranostics
KW - antibodies
KW - targeted delivery
N2 - Conventional cancer treatment often effects normal cells and has many side effects. These can be addressed by the use of nanomedicine, especially its platform nanotransporters, in which cytostatic drug can be encapsulated. These nanotransporters can be dispersed in tumors through relatively large pores in tumor blood vessels but not in normal blood vessels. The nanotransporters can be coupled with antibodies, thus allowing targeted delivery of drugs. For this coupling we chose the method using linker, small peptide that interacts with Fc region of IgG antibodies, presenting the antigen binding site facing out. The aim of this experiment was to create, characterize and test a nanotransporter based on apoferritin nanocage with encapsulated doxorubicin, modified with specific antibody. As a linker, heptapeptide HWRGWVC was used. Cysteine has known affinity towards gold, two methods of apoferritin surface modification were proposed and better results were obtained with apoferritin modified with gold nanoparticles. The presence of gold on apoferritin was proved by separation in polyacrylamide gel electrophoresis (PAGE), followed by inductively coupled plasma mass spectrometric (ICP-MS) measurement. Enzyme-linked immunosorbent assay (ELISA) was used to prove the apoferritin ability to specifically bind to target cells. Apoferritin retained its ability to open and release doxorubicin in low pH. This is very convenient, since there is lower pH in tumors due to hypoxemia.
ER -
DOSTALOVA, S., R. KONECNA, I. BLAZKOVA, M. VACULOVICOVA, P. KOPEL, S. KRIZKOVA, Tomáš VACULOVIČ, V. ADAM a R. KIZEK. APOFERRITIN AS A TARGETED DRUG DELIVERY SYSTEM. In Skarpa, P Ryant, P Cerkal, R Polak, O Kovarnik, J. \textit{MENDELNET 2013}. BRNO: MENDEL UNIV BRNO, FAC AGRONOMY. s.~908-912. ISBN~978-80-7375-908-7. 2013.
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