J 2020

Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis

ĎURČ, Pavol, František FORET, Lukáš HOMOLA, Miriam MALÁ, Eva POKOJOVÁ et. al.

Basic information

Original name

Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis

Authors

ĎURČ, Pavol (703 Slovakia, belonging to the institution), František FORET (203 Czech Republic, belonging to the institution), Lukáš HOMOLA (203 Czech Republic, belonging to the institution), Miriam MALÁ (203 Czech Republic, belonging to the institution), Eva POKOJOVÁ (203 Czech Republic), Hana VINOHRADSKÁ (203 Czech Republic), Milan DASTYCH (203 Czech Republic), Dagmar KRAUSOVÁ (203 Czech Republic, belonging to the institution), D. NAGY, O. BEDE, P. DREVINEK, V. SKALICKA and Petr KUBÁŇ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Pediatric Pulmonology, 111 RIVER ST, HOBOKEN 07030-5774, WILEY, 2020, 8755-6863

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30209 Paediatrics

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.039

RIV identification code

RIV/00216224:14740/20:00116334

Organization unit

Central European Institute of Technology

UT WoS

000559584700001

Keywords in English

capillary electrophoresis; cystic fibrosis; diagnosis; ion ratio; screening; skin wipe test; sweat test

Tags

International impact, Reviewed
Změněno: 21/2/2024 12:52, Mgr. Tereza Miškechová

Abstract

V originále

Objective To assess the performance of a newly developed skin wipe test (SWT) for the diagnosis of cystic fibrosis (CF). Study Design Spontaneously formed sweat from the forearm was wiped by a cotton swab moistened with 100 mu L of deionized (DI) water and extracted into 400 mu L of DI water (SWT). The conventional Macroduct sweat test (ST) was performed simultaneously. SWT samples of 114 CF patients, 76 healthy carriers, and 58 controls were analyzed by capillary electrophoresis with contactless conductivity detection and Cl-/K(+)and (Cl- + Na+)/K(+)ion ratios were evaluated. Chloride concentrations from Macroduct ST were analyzed coulometrically. Results Analysis of 248 SWT samples and simultaneous Macroduct ST samples showed comparable method performance. Two ion ratios, Cl-/K(+)and (Cl- + Na+)/K+, from the SWT samples and Cl(-)values from the ST samples were evaluated to diagnose CF. Sensitivity of the SWT method using the Cl-/K(+)ratio (cutoff value 3.9) was 93.9%, compared to 99.1% when using the (Cl- + Na+)/K(+)ratio (cutoff value 5.0) and 98.3% in using Macroduct Cl-(cutoff value higher or equal to 60 mmol/L). The methods' specificities were 97.8%, 94.0%, and 100.0%, respectively. Conclusions The developed SWT method with capillary electrophoretic analysis for CF diagnosis performs comparably to the conventional Macroduct ST. The SWT method is simple, fast, inexpensive, and completely noninvasive. Use of an ion ratio in obtained SWT samples is proposed as a new diagnostic parameter that shows significant promise in CF diagnostics.

Links

LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/0884/2017, interní kód MU
Name: Potní stěry v diagnostice cystické fibrózy (Acronym: CFskin)
Investor: Masaryk University, Category A
NV18-08-00189, research and development project
Name: Nový postup diagnostiky cystické fibrózy založený na iontových poměrech v neinvazivně získaných vzorcích
Investor: Ministry of Health of the CR