KULUS, M., W. KRANC, Michal JEŠETA, P. SUJKA-KORDOWSKA, A. KONWERSKA, S. CIESIOLKA, P. CELICHOWSKI, L. MONCRIEFF, I. KOCHEROVA, M. JOZKOWIAK, J. KULUS, M. WIECZORKIEWICZ, H. PIOTROWSKA-KEMPISTY, M. T. SKOWRONSKI, D. BUKOWSKA, M. MACHATKOVA, S. HANULAKOVA, P. MOZDZIAK, J. M. JASKOWSKI, B. KEMPISTY a P. ANTOSIK. Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro. Genes. Basilej: MDPI, 2020, roč. 11, č. 7, s. 1-27. ISSN 2073-4425. Dostupné z: https://dx.doi.org/10.3390/genes11070815. |
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@article{1677685, author = {Kulus, M. and Kranc, W. and Ješeta, Michal and SujkaandKordowska, P. and Konwerska, A. and Ciesiolka, S. and Celichowski, P. and Moncrieff, L. and Kocherova, I. and Jozkowiak, M. and Kulus, J. and Wieczorkiewicz, M. and PiotrowskaandKempisty, H. and Skowronski, M. T. and Bukowska, D. and Machatkova, M. and Hanulakova, S. and Mozdziak, P. and Jaskowski, J. M. and Kempisty, B. and Antosik, P.}, article_location = {Basilej}, article_number = {7}, doi = {http://dx.doi.org/10.3390/genes11070815}, keywords = {pig; oocyte maturation; microarray; cortical granule; molecular markers}, language = {eng}, issn = {2073-4425}, journal = {Genes}, title = {Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro}, url = {https://www.mdpi.com/2073-4425/11/7/815}, volume = {11}, year = {2020} }
TY - JOUR ID - 1677685 AU - Kulus, M. - Kranc, W. - Ješeta, Michal - Sujka-Kordowska, P. - Konwerska, A. - Ciesiolka, S. - Celichowski, P. - Moncrieff, L. - Kocherova, I. - Jozkowiak, M. - Kulus, J. - Wieczorkiewicz, M. - Piotrowska-Kempisty, H. - Skowronski, M. T. - Bukowska, D. - Machatkova, M. - Hanulakova, S. - Mozdziak, P. - Jaskowski, J. M. - Kempisty, B. - Antosik, P. PY - 2020 TI - Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro JF - Genes VL - 11 IS - 7 SP - 1-27 EP - 1-27 PB - MDPI SN - 20734425 KW - pig KW - oocyte maturation KW - microarray KW - cortical granule KW - molecular markers UR - https://www.mdpi.com/2073-4425/11/7/815 L2 - https://www.mdpi.com/2073-4425/11/7/815 N2 - Polyspermia is an adverse phenomenon during mammalian fertilization when more than one sperm fuses with a single oocyte. The egg cell is prepared to prevent polyspermia by, among other ways, producing cortical granules (CGs), which are specialized intracellular structures containing enzymes that aim to harden the zona pellucida and block the fusion of subsequent sperm. This work focused on exploring the expression profile of genes that may be associated with cortical reactions, and evaluated the distribution of CGs in immature oocytes and the peripheral density of CGs in mature oocytes. Oocytes were isolated and then processed for in vitro maturation (IVM). Transcriptomic analysis of genes belonging to five ontological groups has been conducted. Six genes showed increased expression after IVM (ARHGEF2,MAP1B,CXCL12,FN1,DAB2, andSOX9), while the majority of genes decreased expression after IVM. Using CG distribution analysis in immature oocytes, movement towards the cortical zone of the oocyte during meiotic competence acquisition was observed. CGs peripheral density decreased with the rise in meiotic competence during the IVM process. The current results reveal important new insights into the in vitro maturation of oocytes. Our results may serve as a basis for further studies to investigate the cortical reaction of oocytes. ER -
KULUS, M., W. KRANC, Michal JEŠETA, P. SUJKA-KORDOWSKA, A. KONWERSKA, S. CIESIOLKA, P. CELICHOWSKI, L. MONCRIEFF, I. KOCHEROVA, M. JOZKOWIAK, J. KULUS, M. WIECZORKIEWICZ, H. PIOTROWSKA-KEMPISTY, M. T. SKOWRONSKI, D. BUKOWSKA, M. MACHATKOVA, S. HANULAKOVA, P. MOZDZIAK, J. M. JASKOWSKI, B. KEMPISTY a P. ANTOSIK. Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro. \textit{Genes}. Basilej: MDPI, 2020, roč.~11, č.~7, s.~1-27. ISSN~2073-4425. Dostupné z: https://dx.doi.org/10.3390/genes11070815.
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