Onset of calciotropic receptors during the initiation of
mandibular/alveolar bone formation

J 2020

Onset of calciotropic receptors during the initiation of mandibular/alveolar bone formation

BOBEK, J, V ORALOVA, H LESOT, Adéla KRATOCHVÍLOVÁ, J DOUBEK et. al.

Základní údaje

Originální název

Onset of calciotropic receptors during the initiation of mandibular/alveolar bone formation

Autoři

BOBEK, J, V ORALOVA, H LESOT, Adéla KRATOCHVÍLOVÁ, J DOUBEK a E MATALOVA

Vydání

Annals of Anatomy - Anatomischer Anzeiger, Jena, Elsevier, 2020, 0940-9602

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.698

DOI

http://dx.doi.org/10.1016/j.aanat.2019.151427

UT WoS

000539110300015

Klíčová slova anglicky

Osteogenesis; Receptors; Parathormone; Activated vitamin D; Calcitonin

Štítky

RIV ne

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 9. 2020 09:43, Mgr. Adéla Kratochvílová, Ph.D.

Anotace

V originále

Mandibular/alveolar (m/a) bone, as a component of the periodontal apparatus, allows for the proper tooth anchorage and function of dentition. Bone formation around the tooth germs starts prenatally and, in the mouse model, the mesenchymal condensation turns into a complex vascularized bone (containing osteo-blasts, -cytes, -clasts) within only two days. This very short but critical period is characterized by synchronized cellular and molecular events. The m/a bone, as others, is subjected to endocrine regulations. This not only requires vasculature to allow the circulation of active molecules (ligands), but also the expression of corresponding cell receptors to define target tissues. This contribution aimed at following the dynamics of calciotropic receptorsexpres sion during morphological transformation of a mesenchymal condensation into the initial m/a bone structure. Receptors for all three calciotropic systemic regulators: parathormone, calcitonin and activated vitamin D (calcitriol), were localized on serial histological sections using immunochemistry and their relative expression was quantified by q-PCR. The onset of calciotropic receptors was followed along with bone cell differentiation (as checked using osteocalcin, sclerostin, RANK and TRAP) and vascularization (CD31) during mouse prenatal/embryonic (E) days 13-15 and 18. Additionally, the timing of calciotropic receptor appearance was compared with that of estrogen receptors (ESR1, ESR2). PTH receptor (PTH1r) appeared in the bone already at E13, when the first osteocalcin-positive cells were detected within the mesenchymal condensation forming the bone anlage. At this stage, blood vessels were only lining the condensation. At E14, the osteoblasts started to express the receptor for activated vitamin D (VDR). At this stage, the vasculature just penetrated the forming bone. On the same day, the first TRAP-positive (but not yet multinucleated) osteoclastic cells were identified. However, calcitonin receptor was detected only one day later. The first Sost-positive osteocytes, present at E15, were PTH1r and VDR positive. ESR1 almost copied the expression pattern of PTH1r, and ESR2 appearance was similar with VDR with a significant increase between E15 and E18. This report focuses on the in vivo situation and links morphological transformation of the mesenchymal cell condensation into a bone structure with dynamics of cell differentiation/maturation, vascularization and onset of receptors for calciotropic endocrine signalling in developing m/a bone. (C) 2019 Elsevier GmbH. All rights reserved.

Citovat

BOBEK, J, V ORALOVA, H LESOT, Adéla KRATOCHVÍLOVÁ, J DOUBEK a E MATALOVA. Onset of calciotropic receptors during the initiation of mandibular/alveolar bone formation. Annals of Anatomy - Anatomischer Anzeiger. Jena: Elsevier, 2020, roč. 227, 9 s. ISSN 0940-9602. Dostupné z: https://dx.doi.org/10.1016/j.aanat.2019.151427.

@article{1678921,
   author = {Bobek, J and Oralova, V and Lesot, H and Kratochvílová, Adéla and Doubek, J and Matalova, E},
   article_location = {Jena},
   doi = {http://dx.doi.org/10.1016/j.aanat.2019.151427},
   keywords = {Osteogenesis; Receptors; Parathormone; Activated vitamin D; Calcitonin},
   language = {eng},
   issn = {0940-9602},
   journal = {Annals of Anatomy - Anatomischer Anzeiger},
   title = {Onset of calciotropic receptors during the initiation of mandibular/alveolar bone formation},
   volume = {227},
   year = {2020}
}

TY  - JOUR
ID  - 1678921
AU  - Bobek, J - Oralova, V - Lesot, H - Kratochvílová, Adéla - Doubek, J - Matalova, E
PY  - 2020
TI  - Onset of calciotropic receptors during the initiation of mandibular/alveolar bone formation
JF  - Annals of Anatomy - Anatomischer Anzeiger
VL  - 227
PB  - Elsevier
SN  - 09409602
KW  - Osteogenesis
KW  - Receptors
KW  - Parathormone
KW  - Activated vitamin D
KW  - Calcitonin
N2  - Mandibular/alveolar (m/a) bone, as a component of the periodontal apparatus, allows for the proper tooth anchorage and function of dentition. Bone formation around the tooth germs starts prenatally and, in the mouse model, the mesenchymal condensation turns into a complex vascularized bone (containing osteo-blasts, -cytes, -clasts) within only two days. This very short but critical period is characterized by synchronized cellular and molecular events. The m/a bone, as others, is subjected to endocrine regulations. This not only requires vasculature to allow the circulation of active molecules (ligands), but also the expression of corresponding cell receptors to define target tissues. This contribution aimed at following the dynamics of calciotropic receptorsexpres sion during morphological transformation of a mesenchymal condensation into the initial m/a bone structure. Receptors for all three calciotropic systemic regulators: parathormone, calcitonin and activated vitamin D (calcitriol), were localized on serial histological sections using immunochemistry and their relative expression was quantified by q-PCR. The onset of calciotropic receptors was followed along with bone cell differentiation (as checked using osteocalcin, sclerostin, RANK and TRAP) and vascularization (CD31) during mouse prenatal/embryonic (E) days 13-15 and 18. Additionally, the timing of calciotropic receptor appearance was compared with that of estrogen receptors (ESR1, ESR2). PTH receptor (PTH1r) appeared in the bone already at E13, when the first osteocalcin-positive cells were detected within the mesenchymal condensation forming the bone anlage. At this stage, blood vessels were only lining the condensation. At E14, the osteoblasts started to express the receptor for activated vitamin D (VDR). At this stage, the vasculature just penetrated the forming bone. On the same day, the first TRAP-positive (but not yet multinucleated) osteoclastic cells were identified. However, calcitonin receptor was detected only one day later. The first Sost-positive osteocytes, present at E15, were PTH1r and VDR positive. ESR1 almost copied the expression pattern of PTH1r, and ESR2 appearance was similar with VDR with a significant increase between E15 and E18. This report focuses on the in vivo situation and links morphological transformation of the mesenchymal cell condensation into a bone structure with dynamics of cell differentiation/maturation, vascularization and onset of receptors for calciotropic endocrine signalling in developing m/a bone. (C) 2019 Elsevier GmbH. All rights reserved.
ER  -

BOBEK, J, V ORALOVA, H LESOT, Adéla KRATOCHVÍLOVÁ, J DOUBEK a E MATALOVA. Onset of calciotropic receptors during the initiation of mandibular/alveolar bone formation. \textit{Annals of Anatomy - Anatomischer Anzeiger}. Jena: Elsevier, 2020, roč.~227, 9 s. ISSN~0940-9602. Dostupné z: https://dx.doi.org/10.1016/j.aanat.2019.151427.

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