Two Reliable Methodical Approaches for Non-Invasive RHD
Genotyping of a Fetus from Maternal Plasma

J 2020

Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma

BOHMOVA, Jana, Marek LUBUSKY, Iva HOLUSKOVA, Martina STUDNICKOVA, Romana KRATOCHVILOVA et. al.

Základní údaje

Originální název

Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma

Autoři

BOHMOVA, Jana (203 Česká republika), Marek LUBUSKY (203 Česká republika), Iva HOLUSKOVA (203 Česká republika), Martina STUDNICKOVA (203 Česká republika), Romana KRATOCHVILOVA (203 Česká republika), Eva KREJCIRIKOVA (203 Česká republika), Veronika DURDOVA (203 Česká republika), Tereza KRATOCHVILOVA (203 Česká republika), Ladislav DUŠEK (203 Česká republika, domácí), Martin PROCHAZKA (203 Česká republika) a Radek VODICKA (203 Česká republika, garant)

Vydání

Diagnostics, Basel, MDPI, 2020, 2075-4418

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30218 General and internal medicine

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.706

Kód RIV

RIV/00216224:14110/20:00116484

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/diagnostics10080564

UT WoS

000568020600001

Klíčová slova anglicky

non-invasive fetal genotyping; RHDgene; cell-free fetal DNA; real-time PCR; QF PCR; Rh blood group system; red blood cell alloimmunization; hemolytic disease of the fetus and newborn

Štítky

14119612, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 9. 2020 07:08, Mgr. Tereza Miškechová

Anotace

V originále

Noninvasive fetalRHDgenotyping is an important tool for predicting RhD incompatibility between a pregnant woman and a fetus. This study aimed to assess a methodological approach other than the commonly used one for noninvasive fetalRHDgenotyping on a representative set of RhD-negative pregnant women. The methodology must be accurate, reliable, and broadly available for implementation into routine clinical practice. A total of 337 RhD-negative pregnant women from the Czech Republic region were tested in this study. The fetalRHDgenotype was assessed using two methods: real-time PCR and endpoint quantitative fluorescent (QF) PCR. We used exon-7-specific primers from theRHDgene, along with internal controls. Plasma samples were analyzed and measured in four/two parallel reactions to determine the accuracy of theRHDgenotyping. TheRHDgenotype was verified using DNA analysis from a newborn buccal swab. Both methods showed an excellent ability to predict theRHDgenotype. Real-time PCR achieved its greatest accuracy of 98.6% (97.1% sensitivity and 100% specificity (95% CI)) if all four PCRs were positive/negative. The QF PCR method also achieved its greatest accuracy of 99.4% (100% sensitivity and 98.6% specificity (95% CI)) if all the measurements were positive/negative. Both real-time PCR and QF PCR were reliable methods for precisely assessing the fetalRHDallele from the plasma of RhD-negative pregnant women.

Citovat

BOHMOVA, Jana, Marek LUBUSKY, Iva HOLUSKOVA, Martina STUDNICKOVA, Romana KRATOCHVILOVA, Eva KREJCIRIKOVA, Veronika DURDOVA, Tereza KRATOCHVILOVA, Ladislav DUŠEK, Martin PROCHAZKA a Radek VODICKA. Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma. Diagnostics. Basel: MDPI, 2020, roč. 10, č. 8, s. 1-15. ISSN 2075-4418. Dostupné z: https://dx.doi.org/10.3390/diagnostics10080564.

@article{1680420,
   author = {Bohmova, Jana and Lubusky, Marek and Holuskova, Iva and Studnickova, Martina and Kratochvilova, Romana and Krejcirikova, Eva and Durdova, Veronika and Kratochvilova, Tereza and Dušek, Ladislav and Prochazka, Martin and Vodicka, Radek},
   article_location = {Basel},
   article_number = {8},
   doi = {http://dx.doi.org/10.3390/diagnostics10080564},
   keywords = {non-invasive fetal genotyping; RHDgene; cell-free fetal DNA; real-time PCR; QF PCR; Rh blood group system; red blood cell alloimmunization; hemolytic disease of the fetus and newborn},
   language = {eng},
   issn = {2075-4418},
   journal = {Diagnostics},
   title = {Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma},
   url = {https://www.mdpi.com/2075-4418/10/8/564},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1680420
AU  - Bohmova, Jana - Lubusky, Marek - Holuskova, Iva - Studnickova, Martina - Kratochvilova, Romana - Krejcirikova, Eva - Durdova, Veronika - Kratochvilova, Tereza - Dušek, Ladislav - Prochazka, Martin - Vodicka, Radek
PY  - 2020
TI  - Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma
JF  - Diagnostics
VL  - 10
IS  - 8
SP  - 1-15
EP  - 1-15
PB  - MDPI
SN  - 20754418
KW  - non-invasive fetal genotyping
KW  - RHDgene
KW  - cell-free fetal DNA
KW  - real-time PCR
KW  - QF PCR
KW  - Rh blood group system
KW  - red blood cell alloimmunization
KW  - hemolytic disease of the fetus and newborn
UR  - https://www.mdpi.com/2075-4418/10/8/564
L2  - https://www.mdpi.com/2075-4418/10/8/564
N2  - Noninvasive fetalRHDgenotyping is an important tool for predicting RhD incompatibility between a pregnant woman and a fetus. This study aimed to assess a methodological approach other than the commonly used one for noninvasive fetalRHDgenotyping on a representative set of RhD-negative pregnant women. The methodology must be accurate, reliable, and broadly available for implementation into routine clinical practice. A total of 337 RhD-negative pregnant women from the Czech Republic region were tested in this study. The fetalRHDgenotype was assessed using two methods: real-time PCR and endpoint quantitative fluorescent (QF) PCR. We used exon-7-specific primers from theRHDgene, along with internal controls. Plasma samples were analyzed and measured in four/two parallel reactions to determine the accuracy of theRHDgenotyping. TheRHDgenotype was verified using DNA analysis from a newborn buccal swab. Both methods showed an excellent ability to predict theRHDgenotype. Real-time PCR achieved its greatest accuracy of 98.6% (97.1% sensitivity and 100% specificity (95% CI)) if all four PCRs were positive/negative. The QF PCR method also achieved its greatest accuracy of 99.4% (100% sensitivity and 98.6% specificity (95% CI)) if all the measurements were positive/negative. Both real-time PCR and QF PCR were reliable methods for precisely assessing the fetalRHDallele from the plasma of RhD-negative pregnant women.
ER  -

BOHMOVA, Jana, Marek LUBUSKY, Iva HOLUSKOVA, Martina STUDNICKOVA, Romana KRATOCHVILOVA, Eva KREJCIRIKOVA, Veronika DURDOVA, Tereza KRATOCHVILOVA, Ladislav DUŠEK, Martin PROCHAZKA a Radek VODICKA. Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma. \textit{Diagnostics}. Basel: MDPI, 2020, roč.~10, č.~8, s.~1-15. ISSN~2075-4418. Dostupné z: https://dx.doi.org/10.3390/diagnostics10080564.

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