GOUDARZI, Mehdi, Nobumichi KOBAYASHI, Masoud DADASHI, Roman PANTŮČEK, Mohammad Javad NASIRI, Maryam FAZELI, Ramin POURIRAN, Hossein GOUDARZI, Mirmohammad MIRI, Anahita AMIRPOUR and Sima Sadat SEYEDJAVADI. Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular-Epidemiological Analysis From 2013 to 2018. Frontiers in Microbiology. Lausanne: Frontiers Media SA, 2020, vol. 11, APR 30 2020, p. "663-1"-"663-18", 18 pp. ISSN 1664-302X. Available from: https://dx.doi.org/10.3389/fmicb.2020.00663.
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Basic information
Original name Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular-Epidemiological Analysis From 2013 to 2018
Authors GOUDARZI, Mehdi, Nobumichi KOBAYASHI, Masoud DADASHI, Roman PANTŮČEK (203 Czech Republic, guarantor, belonging to the institution), Mohammad Javad NASIRI, Maryam FAZELI, Ramin POURIRAN, Hossein GOUDARZI, Mirmohammad MIRI, Anahita AMIRPOUR and Sima Sadat SEYEDJAVADI.
Edition Frontiers in Microbiology, Lausanne, Frontiers Media SA, 2020, 1664-302X.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10606 Microbiology
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.640
RIV identification code RIV/00216224:14310/20:00116495
Organization unit Faculty of Science
Doi http://dx.doi.org/10.3389/fmicb.2020.00663
UT WoS 000536196300001
Keywords in English methicillin-resistant S; aureus; methicillin-susceptible S; aureus; inducible resistance; staphylocoagulase; SCCmec; agr allotype; MLST
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 24/2/2021 11:18.
Abstract
The prevalence of Staphylococcus aureus as an aggressive pathogen resistant to multiple antibiotics causing nosocomial and community-acquired infections is increasing with limited therapeutic options. Macrolide-lincosamide streptogramin B (MLSB) family of antibiotics represents an important alternative therapy for staphylococcal infections. This study was conducted over a period of five years from August 2013 to July 2018 to investigate the prevalence and molecular epidemiology in Iran of inducible resistance in S. aureus. In the current study, 126 inducible methicillin-resistant S. aureus (MRSA) (n = 106) and methicillin-sensitive S. aureus (MSSA) (n = 20) isolates were characterized by in vitro susceptibility analysis, resistance and virulence encoding gene distribution, phenotypic and genotypic analysis of biofilm formation, prophage typing, S. aureus protein A locus (spa) typing, staphylocoagulase (SC) typing, staphylococcal cassette chromosome mec (SCCmec) typing, and multilocus sequence typing. Of the 126 isolates, 76 (60.3%) were classified as hospital onset, and 50 (39.7%) were classified as community onset (CO). Biofilm formation was observed in 97 strains (77%). A total of 14 sequence types (STs), 26 spa types, 7 coagulase types, 9 prophage types, 3 agr types (no agr IV), and 9 clonal complexes (CCs) were identified in this study. The prevalence of the inducible MLSB (iMLSB) S. aureus increased from 7.5% (25/335) to 21.7% (38/175) during the study period. The iMLSB MRSA isolates were distributed in nine CCs, whereas the MSSA isolates were less diverse, which mainly belonged to CC22 (7.95%) and CC30 (7.95%). High-level mupirocin-resistant strains belonged to ST85-SCCmec IV/t008 (n = 4), ST5-SCCmec IV/t002 (n = 4), ST239-SCCmec III/t631 (n = 2), and ST8-SCCmec IV/t064 (n = 2) clones, whereas low-level mupirocin-resistant strains belonged to ST15-SCCmec IV/t084 (n = 5), ST239-SCCmec III/t860 (n = 3), and ST22-SCCmec IV/t790 (n = 3) clones. All the fusidic acid-resistant iMLSB isolates were MRSA and belonged to ST15-SCCmec IV/t084 (n = 2), ST239-SCCmec III/t030 (n = 2), ST1-SCCmec V/t6811 (n = 1), ST80-SCCmec IV/t044 (n = 1), and ST59-SCCmec IV/t437 (n = 1). The CC22 that was predominant in 2013-2014 (36% of the isolates) had almost disappeared in 2017-2018, being replaced by the CC8, which represented 39.5% of the 2017-2018 isolates. This is the first description of temporal shifts of iMLSB S. aureus isolates in Iran that identifies predominant clones and treatment options for iMLSB S. aureus-related infections.
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