GOUDARZI, Mehdi, Nobumichi KOBAYASHI, Masoud DADASHI, Roman PANTŮČEK, Mohammad Javad NASIRI, Maryam FAZELI, Ramin POURIRAN, Hossein GOUDARZI, Mirmohammad MIRI, Anahita AMIRPOUR a Sima Sadat SEYEDJAVADI. Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular-Epidemiological Analysis From 2013 to 2018. Frontiers in Microbiology. Lausanne: Frontiers Media SA, 2020, roč. 11, APR 30 2020, s. "663-1"-"663-18", 18 s. ISSN 1664-302X. Dostupné z: https://dx.doi.org/10.3389/fmicb.2020.00663.
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Základní údaje
Originální název Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular-Epidemiological Analysis From 2013 to 2018
Autoři GOUDARZI, Mehdi, Nobumichi KOBAYASHI, Masoud DADASHI, Roman PANTŮČEK (203 Česká republika, garant, domácí), Mohammad Javad NASIRI, Maryam FAZELI, Ramin POURIRAN, Hossein GOUDARZI, Mirmohammad MIRI, Anahita AMIRPOUR a Sima Sadat SEYEDJAVADI.
Vydání Frontiers in Microbiology, Lausanne, Frontiers Media SA, 2020, 1664-302X.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10606 Microbiology
Stát vydavatele Švýcarsko
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 5.640
Kód RIV RIV/00216224:14310/20:00116495
Organizační jednotka Přírodovědecká fakulta
Doi http://dx.doi.org/10.3389/fmicb.2020.00663
UT WoS 000536196300001
Klíčová slova anglicky methicillin-resistant S; aureus; methicillin-susceptible S; aureus; inducible resistance; staphylocoagulase; SCCmec; agr allotype; MLST
Štítky rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Marie Šípková, DiS., učo 437722. Změněno: 24. 2. 2021 11:18.
Anotace
The prevalence of Staphylococcus aureus as an aggressive pathogen resistant to multiple antibiotics causing nosocomial and community-acquired infections is increasing with limited therapeutic options. Macrolide-lincosamide streptogramin B (MLSB) family of antibiotics represents an important alternative therapy for staphylococcal infections. This study was conducted over a period of five years from August 2013 to July 2018 to investigate the prevalence and molecular epidemiology in Iran of inducible resistance in S. aureus. In the current study, 126 inducible methicillin-resistant S. aureus (MRSA) (n = 106) and methicillin-sensitive S. aureus (MSSA) (n = 20) isolates were characterized by in vitro susceptibility analysis, resistance and virulence encoding gene distribution, phenotypic and genotypic analysis of biofilm formation, prophage typing, S. aureus protein A locus (spa) typing, staphylocoagulase (SC) typing, staphylococcal cassette chromosome mec (SCCmec) typing, and multilocus sequence typing. Of the 126 isolates, 76 (60.3%) were classified as hospital onset, and 50 (39.7%) were classified as community onset (CO). Biofilm formation was observed in 97 strains (77%). A total of 14 sequence types (STs), 26 spa types, 7 coagulase types, 9 prophage types, 3 agr types (no agr IV), and 9 clonal complexes (CCs) were identified in this study. The prevalence of the inducible MLSB (iMLSB) S. aureus increased from 7.5% (25/335) to 21.7% (38/175) during the study period. The iMLSB MRSA isolates were distributed in nine CCs, whereas the MSSA isolates were less diverse, which mainly belonged to CC22 (7.95%) and CC30 (7.95%). High-level mupirocin-resistant strains belonged to ST85-SCCmec IV/t008 (n = 4), ST5-SCCmec IV/t002 (n = 4), ST239-SCCmec III/t631 (n = 2), and ST8-SCCmec IV/t064 (n = 2) clones, whereas low-level mupirocin-resistant strains belonged to ST15-SCCmec IV/t084 (n = 5), ST239-SCCmec III/t860 (n = 3), and ST22-SCCmec IV/t790 (n = 3) clones. All the fusidic acid-resistant iMLSB isolates were MRSA and belonged to ST15-SCCmec IV/t084 (n = 2), ST239-SCCmec III/t030 (n = 2), ST1-SCCmec V/t6811 (n = 1), ST80-SCCmec IV/t044 (n = 1), and ST59-SCCmec IV/t437 (n = 1). The CC22 that was predominant in 2013-2014 (36% of the isolates) had almost disappeared in 2017-2018, being replaced by the CC8, which represented 39.5% of the 2017-2018 isolates. This is the first description of temporal shifts of iMLSB S. aureus isolates in Iran that identifies predominant clones and treatment options for iMLSB S. aureus-related infections.
VytisknoutZobrazeno: 1. 9. 2024 06:19