miR-183/96/182 cluster is an important morphogenetic factor
targetingPAX6expression in differentiating human retinal
organoids

J 2020

miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids

PEŠKOVÁ, Lucie; Denisa JURČÍKOVÁ; Tereza VÁŇOVÁ; Jan KŘIVÁNEK; Michaela CAPANDOVÁ et. al.

Základní údaje

Originální název

miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids

Autoři

Vydání

Stem Cells, Hoboken, Wiley-Blackwell, 2020, 1066-5099

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.277

Kód RIV

RIV/00216224:14110/20:00116545

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1002/stem.3272

UT WoS

000569493800001

EID Scopus

2-s2.0-85090968273

Klíčová slova anglicky

microRNAs; organoids; pluripotent stem cells; retina

Štítky

14110517, CF CELLIM, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 10. 2024 17:14, Ing. Martina Blahová

Anotace

V originále

MicroRNAs (miRNAs), a class of small, noncoding RNA molecules represent important regulators of gene expression. Recent reports have implicated their role in the cell specification process acting as "fine-tuners" to ensure the precise gene expression at the specific stage of cell differentiation. Here, we used retinal organoids differentiated from human pluripotent stem cells (hPSCs) as a model to closely investigate the role of a sensory organ-specific and evolutionary conserved miR-183/96/182 cluster. Using a miRNA tough decoy approach, we inhibited the miR-183/96/182 cluster in hPSCs. Inhibition of the miRNA cluster resulted in an increased expansion of neuroepithelium leading to abnormal "bulged" neural retina in organoids, associated with upregulation of neural-specific and retinal-specific genes. Importantly, we identifiedPAX6, a well-known essential gene in neuroectoderm specification, as a target of the miR-183/96/182 cluster members. Taken together, the miR-183/96/182 cluster not only represents an important regulator ofPAX6expression, but it also plays a crucial role in retinal tissue morphogenesis.

Návaznosti

MUNI/G/1391/2018, interní kód MU

Název: At the Cross-road of Magnetic Fields and Light: A New Perspective of Cell Clock Control.

Investor: Masarykova univerzita, At the Cross-road of Magnetic Fields and Light: A New Perspective of Cell Clock Control., INTERDISCIPLINARY - Mezioborové výzkumné projekty

ROZV/28/LF20/2020, interní kód MU

Název: Využiti retinálních organoidů pro studium vlivu fotostimulace na lidskou retinu

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Využití retinálních organoidů pro studium vlivu fotostimulace na lidskou retinu, Interní rozvojové projekty

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

Citovat

PEŠKOVÁ, Lucie; Denisa JURČÍKOVÁ; Tereza VÁŇOVÁ; Jan KŘIVÁNEK; Michaela CAPANDOVÁ; Zuzana ŠRÁMKOVÁ; Jana ŠEBESTÍKOVÁ; Magdaléna KOLOUŠKOVÁ; Hana KOTASOVÁ; Libor STREIT a Tomáš BÁRTA. miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids. Stem Cells. Hoboken: Wiley-Blackwell, 2020, roč. 38, č. 12, s. 1557-1567. ISSN 1066-5099. Dostupné z: https://doi.org/10.1002/stem.3272.

@article{1682016,
   author = {Pešková, Lucie and Jurčíková, Denisa and Váňová, Tereza and Křivánek, Jan and Capandová, Michaela and Šrámková, Zuzana and Šebestíková, Jana and Koloušková, Magdaléna and Kotasová, Hana and Streit, Libor and Bárta, Tomáš},
   article_location = {Hoboken},
   article_number = {12},
   doi = {https://doi.org/10.1002/stem.3272},
   keywords = {microRNAs; organoids; pluripotent stem cells; retina},
   language = {eng},
   issn = {1066-5099},
   journal = {Stem Cells},
   title = {miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids},
   url = {https://stemcellsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/stem.3272},
   volume = {38},
   year = {2020}
}

TY  - JOUR
ID  - 1682016
AU  - Pešková, Lucie - Jurčíková, Denisa - Váňová, Tereza - Křivánek, Jan - Capandová, Michaela - Šrámková, Zuzana - Šebestíková, Jana - Koloušková, Magdaléna - Kotasová, Hana - Streit, Libor - Bárta, Tomáš
PY  - 2020
TI  - miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids
JF  - Stem Cells
VL  - 38
IS  - 12
SP  - 1557-1567
EP  - 1557-1567
PB  - Wiley-Blackwell
SN  - 10665099
KW  - microRNAs
KW  - organoids
KW  - pluripotent stem cells
KW  - retina
UR  - https://stemcellsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/stem.3272
L2  - https://stemcellsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/stem.3272
N2  - MicroRNAs (miRNAs), a class of small, noncoding RNA molecules represent important regulators of gene expression. Recent reports have implicated their role in the cell specification process acting as "fine-tuners" to ensure the precise gene expression at the specific stage of cell differentiation. Here, we used retinal organoids differentiated from human pluripotent stem cells (hPSCs) as a model to closely investigate the role of a sensory organ-specific and evolutionary conserved miR-183/96/182 cluster. Using a miRNA tough decoy approach, we inhibited the miR-183/96/182 cluster in hPSCs. Inhibition of the miRNA cluster resulted in an increased expansion of neuroepithelium leading to abnormal "bulged" neural retina in organoids, associated with upregulation of neural-specific and retinal-specific genes. Importantly, we identifiedPAX6, a well-known essential gene in neuroectoderm specification, as a target of the miR-183/96/182 cluster members. Taken together, the miR-183/96/182 cluster not only represents an important regulator ofPAX6expression, but it also plays a crucial role in retinal tissue morphogenesis.
ER  -

PEŠKOVÁ, Lucie; Denisa JURČÍKOVÁ; Tereza VÁŇOVÁ; Jan KŘIVÁNEK; Michaela CAPANDOVÁ; Zuzana ŠRÁMKOVÁ; Jana ŠEBESTÍKOVÁ; Magdaléna KOLOUŠKOVÁ; Hana KOTASOVÁ; Libor STREIT a Tomáš BÁRTA. miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids. \textit{Stem Cells}. Hoboken: Wiley-Blackwell, 2020, roč.~38, č.~12, s.~1557-1567. ISSN~1066-5099. Dostupné z: https://doi.org/10.1002/stem.3272.

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