Matrix-assisted laser desorption/ionization mass spectrometry
(MALDI TOF MS) study of Huperzine A, a natural anti-Alzheimer's
disease product, its derivatization and its detection by highly
sensitive laser induced fluorescence (LIF)

J 2007

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI TOF MS) study of Huperzine A, a natural anti-Alzheimer's disease product, its derivatization and its detection by highly sensitive laser induced fluorescence (LIF)

BEN HAMEDA, Azdeen A. Abdullah; Petr TÁBORSKÝ; E. PENA-MENDEZ a Josef HAVEL

Základní údaje

Originální název

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI TOF MS) study of Huperzine A, a natural anti-Alzheimer's disease product, its derivatization and its detection by highly sensitive laser induced fluorescence (LIF)

Autoři

BEN HAMEDA, Azdeen A. Abdullah; Petr TÁBORSKÝ; E. PENA-MENDEZ a Josef HAVEL

Vydání

Talanta, 2007, 0039-9140

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.374

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.talanta.2006.12.010

UT WoS

000246321200065

Klíčová slova anglicky

Huperzine A; derivatization; MALDI TOF MS; CE-LIF

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 10. 2020 09:56, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Huperzine A, a reversible acetylcholinesterase inhibitor for the treatment of Alzheimer disease (HupA), was studied using an (MALDI TOF MS) instrument in MALDI mode. The formation of a HupA dimmer in a vacuum was observed and several matrices were found that were able to inhibit its formation. The structures of the neutral and protonated form of the HupA molecule were calculated and optimized using a Hyperchem program. Detection limit using MALDI TOF MS in the model sample was 5.3 pg. MALDI TOF MS was also applied to the direct detection of the drug in medical preparations and in human serum. The limit of detection in plasma was 14.2 pg with a signal-to-noise ratio of 3:1. However, the sensitivity was not as high as it usually is in MALDI. Therefore, a new method for the derivatization of HupA was developed using fluorescent labelling with rhodamine B isothiocyanate (RBITC). A limit of detection using capillary electrophoresis laser induced fluorescence detection (CE-LIF) equal to 4 x 10(-9) mol l(-1) was reached. (c) 2007 Elsevier B.V. All rights reserved.

Návaznosti

GA525/06/0663, projekt VaV

Název: Sledování chemických změn obilky ječmene po napadení patogeny pro kontrolu a zvýšení kvality ječmene a následných potravinářských produktů

Investor: Grantová agentura ČR, Sledování chemických změn obilky ječmene po napadení patogeny pro kontrolu a zvýšení kvality ječmene a následných potravinářských produktů

Citovat

BEN HAMEDA, Azdeen A. Abdullah; Petr TÁBORSKÝ; E. PENA-MENDEZ a Josef HAVEL. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI TOF MS) study of Huperzine A, a natural anti-Alzheimer's disease product, its derivatization and its detection by highly sensitive laser induced fluorescence (LIF). Talanta. 2007, roč. 72, č. 2, s. 780-784. ISSN 0039-9140. Dostupné z: https://doi.org/10.1016/j.talanta.2006.12.010.

@article{1685069,
   author = {Ben Hameda, Azdeen A. Abdullah and Táborský, Petr and PenaandMendez, E. and Havel, Josef},
   article_number = {2},
   doi = {https://doi.org/10.1016/j.talanta.2006.12.010},
   keywords = {Huperzine A; derivatization; MALDI TOF MS; CE-LIF},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Matrix-assisted laser desorption/ionization mass spectrometry (MALDI TOF MS) study of Huperzine A, a natural anti-Alzheimer's disease product, its derivatization and its detection by highly sensitive laser induced fluorescence (LIF)},
   url = {https://www.sciencedirect.com/science/article/pii/S0039914006007922?via%3Dihub},
   volume = {72},
   year = {2007}
}

TY  - JOUR
ID  - 1685069
AU  - Ben Hameda, Azdeen A. Abdullah - Táborský, Petr - Pena-Mendez, E. - Havel, Josef
PY  - 2007
TI  - Matrix-assisted laser desorption/ionization mass spectrometry (MALDI TOF MS) study of Huperzine A, a natural anti-Alzheimer's disease product, its derivatization and its detection by highly sensitive laser induced fluorescence (LIF)
JF  - Talanta
VL  - 72
IS  - 2
SP  - 780-784
EP  - 780-784
SN  - 00399140
KW  - Huperzine A
KW  - derivatization
KW  - MALDI TOF MS
KW  - CE-LIF
UR  - https://www.sciencedirect.com/science/article/pii/S0039914006007922?via%3Dihub
N2  - Huperzine A, a reversible acetylcholinesterase inhibitor for the treatment of Alzheimer disease (HupA), was studied using an (MALDI TOF MS) instrument in MALDI mode. The formation of a HupA dimmer in a vacuum was observed and several matrices were found that were able to inhibit its formation. The structures of the neutral and protonated form of the HupA molecule were calculated and optimized using a Hyperchem program. Detection limit using MALDI TOF MS in the model sample was 5.3 pg. MALDI TOF MS was also applied to the direct detection of the drug in medical preparations and in human serum. The limit of detection in plasma was 14.2 pg with a signal-to-noise ratio of 3:1. However, the sensitivity was not as high as it usually is in MALDI. Therefore, a new method for the derivatization of HupA was developed using fluorescent labelling with rhodamine B isothiocyanate (RBITC). A limit of detection using capillary electrophoresis laser induced fluorescence detection (CE-LIF) equal to 4 x 10(-9) mol l(-1) was reached. (c) 2007 Elsevier B.V. All rights reserved.
ER  -

BEN HAMEDA, Azdeen A. Abdullah; Petr TÁBORSKÝ; E. PENA-MENDEZ a Josef HAVEL. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI TOF MS) study of Huperzine A, a natural anti-Alzheimer's disease product, its derivatization and its detection by highly sensitive laser induced fluorescence (LIF). \textit{Talanta}. 2007, roč.~72, č.~2, s.~780-784. ISSN~0039-9140. Dostupné z: https://doi.org/10.1016/j.talanta.2006.12.010.

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