Blocking the Nanopores in a Layer of Nonconductive
Nanoparticles: Dominant Effects Therein and Challenges for
Electrochemical Impedimetric Biosensing

J 2020

Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing

SOPOUŠEK, Jakub, Jakub VĚŽNÍK, Petr SKLÁDAL and Karel LACINA

Basic information

Original name

Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing

Authors

SOPOUŠEK, Jakub (203 Czech Republic, belonging to the institution), Jakub VĚŽNÍK (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Karel LACINA (203 Czech Republic, guarantor, belonging to the institution)

Edition

ACS Applied Materials & Interfaces, Washington, D.C. American Chemical Society, 2020, 1944-8244

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 9.229

RIV identification code

RIV/00216224:14740/20:00114473

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1021/acsami.0c02650

UT WoS

000526552100106

Keywords in English

nanopores; biosensing; electrochemical impedance spectroscopy; polystyrene nanoparticles; influence of charge

Abstract

V originále

Blockage of a nanopore by an analyte molecule has emerged as a promising concept for electrochemical biosensing. Nanoporous structures can be formed on the electrode surface simply by packing spherical nanoparticles in a dense planar arrangement. Modification of the nanoparticles with human serum albumin (HSA) and its interaction with the corresponding antibody (anti-HSA) can induce nanopore-blockage which significantly hinders permeation of the redox probe ([Fe(CN6)](4-/3-)). Interfaces of different parameters were studied using Electrochemical Impedance Spectroscopy (EIS), and counterintuitively, the influence of charge of the nanoparticles and other immobilized entities played a substantial role in the measurement. Our study reveals dominant effects including the presence of mixed output signal and resolves corresponding EIS biosensing-related challenges. Consequently, blocking the nanopores was introduced as an efficient technique which enables the application of EIS-based biosensing to real-world analytical issues.

Links

GJ19-16273Y, research and development project

Name: ROS-indukované uvolňování léčiv založené na interakcích ferocenylboronátů

Investor: Czech Science Foundation, ROS-Induced Drug Release Based on Interactions of Ferrocenylboronates

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1252/2019, interní kód MU

Name: Podpora biochemického výzkumu v roce 2020

Investor: Masaryk University, Category A

MUNI/A/1359/2018, interní kód MU

Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2019

Investor: Masaryk University, Category A

90043, large research infrastructures

Name: CIISB

Citovat

SOPOUŠEK, Jakub, Jakub VĚŽNÍK, Petr SKLÁDAL and Karel LACINA. Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing. ACS Applied Materials & Interfaces. Washington, D.C.: American Chemical Society, 2020, vol. 12, No 12, p. 14620-14628. ISSN 1944-8244. Available from: https://dx.doi.org/10.1021/acsami.0c02650.

@article{1694637,
   author = {Sopoušek, Jakub and Věžník, Jakub and Skládal, Petr and Lacina, Karel},
   article_location = {Washington, D.C.},
   article_number = {12},
   doi = {http://dx.doi.org/10.1021/acsami.0c02650},
   keywords = {nanopores; biosensing; electrochemical impedance spectroscopy; polystyrene nanoparticles; influence of charge},
   language = {eng},
   issn = {1944-8244},
   journal = {ACS Applied Materials & Interfaces},
   title = {Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing},
   url = {https://doi.org/10.1021/acsami.0c02650},
   volume = {12},
   year = {2020}
}

TY  - JOUR
ID  - 1694637
AU  - Sopoušek, Jakub - Věžník, Jakub - Skládal, Petr - Lacina, Karel
PY  - 2020
TI  - Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing
JF  - ACS Applied Materials & Interfaces
VL  - 12
IS  - 12
SP  - 14620-14628
EP  - 14620-14628
PB  - American Chemical Society
SN  - 19448244
KW  - nanopores
KW  - biosensing
KW  - electrochemical impedance spectroscopy
KW  - polystyrene nanoparticles
KW  - influence of charge
UR  - https://doi.org/10.1021/acsami.0c02650
L2  - https://doi.org/10.1021/acsami.0c02650
N2  - Blockage of a nanopore by an analyte molecule has emerged as a promising concept for electrochemical biosensing. Nanoporous structures can be formed on the electrode surface simply by packing spherical nanoparticles in a dense planar arrangement. Modification of the nanoparticles with human serum albumin (HSA) and its interaction with the corresponding antibody (anti-HSA) can induce nanopore-blockage which significantly hinders permeation of the redox probe ([Fe(CN6)](4-/3-)). Interfaces of different parameters were studied using Electrochemical Impedance Spectroscopy (EIS), and counterintuitively, the influence of charge of the nanoparticles and other immobilized entities played a substantial role in the measurement. Our study reveals dominant effects including the presence of mixed output signal and resolves corresponding EIS biosensing-related challenges. Consequently, blocking the nanopores was introduced as an efficient technique which enables the application of EIS-based biosensing to real-world analytical issues.
ER  -

SOPOUŠEK, Jakub, Jakub VĚŽNÍK, Petr SKLÁDAL and Karel LACINA. Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing. \textit{ACS Applied Materials \&{} Interfaces}. Washington, D.C.: American Chemical Society, 2020, vol.~12, No~12, p.~14620-14628. ISSN~1944-8244. Available from: https://dx.doi.org/10.1021/acsami.0c02650.

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