Multichannel separation device with parallel electrochemical
detection

J 2020

Multichannel separation device with parallel electrochemical detection

KOMENDOVÁ, Martina; Suhas NAWADA; Radovan METELKA; Peter J. SCHOENMAKERS; Jiří URBAN et al.

Základní údaje

Originální název

Multichannel separation device with parallel electrochemical detection

Autoři

KOMENDOVÁ, Martina; Suhas NAWADA; Radovan METELKA; Peter J. SCHOENMAKERS a Jiří URBAN

Vydání

Journal of Chromatography A, Amsterdam, Elsevier, 2020, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.759

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/20:00114497

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

3D printing; Multi-channel device; Dopamine metabolism; Electrochemical detection; Polymer monoliths; Titanium

Štítky

14313010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 11. 2020 09:29, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

A device with four parallel channels was designed and manufactured by 3D printing in titanium. A simple experimental setup allowed splitting of the mobile phase in four parallel streams, such that a single sample could be analysed four times simultaneously. The four capillary channels were filled with a monolithic stationary phase, prepared using a zwitterionic functional monomer in combination with various dimethacrylate cross-linkers. The resulting stationary phases were applicable in both reversed-phase and hydrophilic-interaction retention mechanisms. The mobile-phase composition was optimized by means of a window diagram so as to obtain the highest possible resolution of dopamine precursors and metabolites on all columns. Miniaturized electrochemical detectors with carbon fibres as working electrodes and silver micro-wires as reference electrodes were integrated in the device at the end of each column. Experimental separations were successfully compared with those predicted by a three-parameter retention model. Finally, dopamine was determined in human urine to further confirm applicability of the developed device.

Návaznosti

GA17-11252S, projekt VaV

Název: Polymerní monolitické stacionární fáze s kontrolovanou selektivitou

Investor: Grantová agentura ČR, Polymerní monolitické stacionární fáze s kontrolovanou selektivitou

Citovat

KOMENDOVÁ, Martina; Suhas NAWADA; Radovan METELKA; Peter J. SCHOENMAKERS a Jiří URBAN. Multichannel separation device with parallel electrochemical detection. Journal of Chromatography A. Amsterdam: Elsevier, 2020, roč. 1610, JAN 11 2020, s. 1-9. ISSN 0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2019.460537.

@article{1698696,
   author = {Komendová, Martina and Nawada, Suhas and Metelka, Radovan and Schoenmakers, Peter J. and Urban, Jiří},
   article_location = {Amsterdam},
   article_number = {JAN 11 2020},
   doi = {https://doi.org/10.1016/j.chroma.2019.460537},
   keywords = {3D printing; Multi-channel device; Dopamine metabolism; Electrochemical detection; Polymer monoliths; Titanium},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Multichannel separation device with parallel electrochemical detection},
   url = {https://doi.org/10.1016/j.chroma.2019.460537},
   volume = {1610},
   year = {2020}
}

TY  - JOUR
ID  - 1698696
AU  - Komendová, Martina - Nawada, Suhas - Metelka, Radovan - Schoenmakers, Peter J. - Urban, Jiří
PY  - 2020
TI  - Multichannel separation device with parallel electrochemical detection
JF  - Journal of Chromatography A
VL  - 1610
IS  - JAN 11 2020
SP  - 1-9
EP  - 1-9
PB  - Elsevier
SN  - 00219673
KW  - 3D printing
KW  - Multi-channel device
KW  - Dopamine metabolism
KW  - Electrochemical detection
KW  - Polymer monoliths
KW  - Titanium
UR  - https://doi.org/10.1016/j.chroma.2019.460537
L2  - https://doi.org/10.1016/j.chroma.2019.460537
N2  - A device with four parallel channels was designed and manufactured by 3D printing in titanium. A simple experimental setup allowed splitting of the mobile phase in four parallel streams, such that a single sample could be analysed four times simultaneously. The four capillary channels were filled with a monolithic stationary phase, prepared using a zwitterionic functional monomer in combination with various dimethacrylate cross-linkers. The resulting stationary phases were applicable in both reversed-phase and hydrophilic-interaction retention mechanisms. The mobile-phase composition was optimized by means of a window diagram so as to obtain the highest possible resolution of dopamine precursors and metabolites on all columns. Miniaturized electrochemical detectors with carbon fibres as working electrodes and silver micro-wires as reference electrodes were integrated in the device at the end of each column. Experimental separations were successfully compared with those predicted by a three-parameter retention model. Finally, dopamine was determined in human urine to further confirm applicability of the developed device.
ER  -

KOMENDOVÁ, Martina; Suhas NAWADA; Radovan METELKA; Peter J. SCHOENMAKERS a Jiří URBAN. Multichannel separation device with parallel electrochemical detection. \textit{Journal of Chromatography A}. Amsterdam: Elsevier, 2020, roč.~1610, JAN 11 2020, s.~1-9. ISSN~0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2019.460537.

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