Multichannel separation device with parallel electrochemical
detection

J 2020

Multichannel separation device with parallel electrochemical detection

KOMENDOVÁ, Martina, Suhas NAWADA, Radovan METELKA, Peter J. SCHOENMAKERS, Jiří URBAN et. al.

Základní údaje

Originální název

Multichannel separation device with parallel electrochemical detection

Autoři

KOMENDOVÁ, Martina (203 Česká republika, domácí), Suhas NAWADA, Radovan METELKA, Peter J. SCHOENMAKERS a Jiří URBAN (203 Česká republika, garant, domácí)

Vydání

Journal of Chromatography A, Amsterdam, Elsevier, 2020, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.759

Kód RIV

RIV/00216224:14310/20:00114497

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.chroma.2019.460537

UT WoS

000509816200013

Klíčová slova anglicky

3D printing; Multi-channel device; Dopamine metabolism; Electrochemical detection; Polymer monoliths; Titanium

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 11. 2020 09:29, Mgr. Marie Šípková, DiS.

Anotace

V originále

A device with four parallel channels was designed and manufactured by 3D printing in titanium. A simple experimental setup allowed splitting of the mobile phase in four parallel streams, such that a single sample could be analysed four times simultaneously. The four capillary channels were filled with a monolithic stationary phase, prepared using a zwitterionic functional monomer in combination with various dimethacrylate cross-linkers. The resulting stationary phases were applicable in both reversed-phase and hydrophilic-interaction retention mechanisms. The mobile-phase composition was optimized by means of a window diagram so as to obtain the highest possible resolution of dopamine precursors and metabolites on all columns. Miniaturized electrochemical detectors with carbon fibres as working electrodes and silver micro-wires as reference electrodes were integrated in the device at the end of each column. Experimental separations were successfully compared with those predicted by a three-parameter retention model. Finally, dopamine was determined in human urine to further confirm applicability of the developed device.

Návaznosti

GA17-11252S, projekt VaV

Název: Polymerní monolitické stacionární fáze s kontrolovanou selektivitou

Investor: Grantová agentura ČR, Polymerní monolitické stacionární fáze s kontrolovanou selektivitou

Citovat

KOMENDOVÁ, Martina, Suhas NAWADA, Radovan METELKA, Peter J. SCHOENMAKERS a Jiří URBAN. Multichannel separation device with parallel electrochemical detection. Journal of Chromatography A. Amsterdam: Elsevier, 2020, roč. 1610, JAN 11 2020, s. 1-9. ISSN 0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2019.460537.

@article{1698696,
   author = {Komendová, Martina and Nawada, Suhas and Metelka, Radovan and Schoenmakers, Peter J. and Urban, Jiří},
   article_location = {Amsterdam},
   article_number = {JAN 11 2020},
   doi = {http://dx.doi.org/10.1016/j.chroma.2019.460537},
   keywords = {3D printing; Multi-channel device; Dopamine metabolism; Electrochemical detection; Polymer monoliths; Titanium},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Multichannel separation device with parallel electrochemical detection},
   url = {https://doi.org/10.1016/j.chroma.2019.460537},
   volume = {1610},
   year = {2020}
}

TY  - JOUR
ID  - 1698696
AU  - Komendová, Martina - Nawada, Suhas - Metelka, Radovan - Schoenmakers, Peter J. - Urban, Jiří
PY  - 2020
TI  - Multichannel separation device with parallel electrochemical detection
JF  - Journal of Chromatography A
VL  - 1610
IS  - JAN 11 2020
SP  - 1-9
EP  - 1-9
PB  - Elsevier
SN  - 00219673
KW  - 3D printing
KW  - Multi-channel device
KW  - Dopamine metabolism
KW  - Electrochemical detection
KW  - Polymer monoliths
KW  - Titanium
UR  - https://doi.org/10.1016/j.chroma.2019.460537
L2  - https://doi.org/10.1016/j.chroma.2019.460537
N2  - A device with four parallel channels was designed and manufactured by 3D printing in titanium. A simple experimental setup allowed splitting of the mobile phase in four parallel streams, such that a single sample could be analysed four times simultaneously. The four capillary channels were filled with a monolithic stationary phase, prepared using a zwitterionic functional monomer in combination with various dimethacrylate cross-linkers. The resulting stationary phases were applicable in both reversed-phase and hydrophilic-interaction retention mechanisms. The mobile-phase composition was optimized by means of a window diagram so as to obtain the highest possible resolution of dopamine precursors and metabolites on all columns. Miniaturized electrochemical detectors with carbon fibres as working electrodes and silver micro-wires as reference electrodes were integrated in the device at the end of each column. Experimental separations were successfully compared with those predicted by a three-parameter retention model. Finally, dopamine was determined in human urine to further confirm applicability of the developed device.
ER  -

KOMENDOVÁ, Martina, Suhas NAWADA, Radovan METELKA, Peter J. SCHOENMAKERS a Jiří URBAN. Multichannel separation device with parallel electrochemical detection. \textit{Journal of Chromatography A}. Amsterdam: Elsevier, 2020, roč.~1610, JAN 11 2020, s.~1-9. ISSN~0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2019.460537.

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