| RADASZKIEWICZ, Katarzyna Anna, Deborah BECKEROVÁ, Lucie WOLOSZCZUKOVÁ, Tomasz Witold RADASZKIEWICZ, Petra LESÁKOVÁ, Olga VONDÁLOVÁ BLANÁŘOVÁ, Lukáš KUBALA, Petr HUMPOLÍČEK a Jiří PACHERNÍK. 12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling. Scientific reports. Berlin: Nature Research, 2020, roč. 10, č. 1, s. 1-13. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-020-73074-4. |
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@article{1703696,
author = {Radaszkiewicz, Katarzyna Anna and Beckerová, Deborah and Woloszczuková, Lucie and Radaszkiewicz, Tomasz Witold and Lesáková, Petra and Vondálová Blanářová, Olga and Kubala, Lukáš and Humpolíček, Petr and Pacherník, Jiří},
article_location = {Berlin},
article_number = {1},
doi = {http://dx.doi.org/10.1038/s41598-020-73074-4},
keywords = {Cell biology; Developmental biology; Stem cells},
language = {eng},
issn = {2045-2322},
journal = {Scientific reports},
title = {12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling},
url = {https://doi.org/10.1038/s41598-020-73074-4},
volume = {10},
year = {2020}
}
TY - JOUR
ID - 1703696
AU - Radaszkiewicz, Katarzyna Anna - Beckerová, Deborah - Woloszczuková, Lucie - Radaszkiewicz, Tomasz Witold - Lesáková, Petra - Vondálová Blanářová, Olga - Kubala, Lukáš - Humpolíček, Petr - Pacherník, Jiří
PY - 2020
TI - 12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling
JF - Scientific reports
VL - 10
IS - 1
SP - 1-13
EP - 1-13
PB - Nature Research
SN - 20452322
KW - Cell biology
KW - Developmental biology
KW - Stem cells
UR - https://doi.org/10.1038/s41598-020-73074-4
L2 - https://doi.org/10.1038/s41598-020-73074-4
N2 - 12-O-Tetradecanoylphorbol-13-acetate (TPA) is the most widely used diacylglycerol (DAG) mimetic agent and inducer of protein kinase C (PKC)-mediated cellular response in biomedical studies. TPA has been proposed as a pluripotent cell differentiation factor, but results obtained have been inconsistent. In the present study we show that TPA can be applied as a cardiomyogenesis-promoting factor for the differentiation of mouse embryonic stem (mES) cells in vitro. The mechanism of TPA action is mediated by the induction of extracellular signal-regulated kinase (ERK) activity and the subsequent phosphorylation of GATA4 transcription factor. Interestingly, general mitogens (FGF, EGF, VEGF and serum) or canonical WNT signalling did not mimic the effect of TPA. Moreover, on the basis of our results, we postulate that a TPA-sensitive population of cardiac progenitor cells exists at a certain time point (after days 6-8 of the differentiation protocol) and that the proposed treatment can be used to increase the multiplication of ES cell-derived cardiomyocytes.
ER -
RADASZKIEWICZ, Katarzyna Anna, Deborah BECKEROVÁ, Lucie WOLOSZCZUKOVÁ, Tomasz Witold RADASZKIEWICZ, Petra LESÁKOVÁ, Olga VONDÁLOVÁ BLANÁŘOVÁ, Lukáš KUBALA, Petr HUMPOLÍČEK a Jiří PACHERNÍK. 12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling. \textit{Scientific reports}. Berlin: Nature Research, 2020, roč.~10, č.~1, s.~1-13. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-020-73074-4.
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