Human-like telomeres in Zostera marina reveal a mode of
transition from the plant to the human telomeric sequences

J 2020

Human-like telomeres in Zostera marina reveal a mode of transition from the plant to the human telomeric sequences

PEŠKA, Vratislav, Martin MÁTL, Terezie MANDÁKOVÁ, Daniel VITALES, Petr FAJKUS et. al.

Basic information

Original name

Human-like telomeres in Zostera marina reveal a mode of transition from the plant to the human telomeric sequences

Authors

PEŠKA, Vratislav (guarantor), Martin MÁTL (203 Czech Republic, belonging to the institution), Terezie MANDÁKOVÁ (203 Czech Republic, belonging to the institution), Daniel VITALES, Petr FAJKUS (203 Czech Republic, belonging to the institution), Jiří FAJKUS (203 Czech Republic, belonging to the institution) and Sonia GARCIA (724 Spain)

Edition

Journal of Experimental Botany, Oxford, Oxford University Press, 2020, 0022-0957

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.992

RIV identification code

RIV/00216224:14740/20:00114533

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/jxb/eraa293

UT WoS

000577075400009

Keywords in English

Animal telomeres; chromosome; cytogenetics; plant telomeres; Tandem Repeats Finder; telomerase RNA; telomere template

Abstract

V originále

A previous study describing the genome of Zostera marina, the most widespread seagrass in the Northern hemisphere, revealed some genomic signatures of adaptation to the aquatic environment such as the loss of stomatal genes, while other functions such as an algal-like cell wall composition were acquired. Beyond these, the genome structure and organization were comparable with those of the majority of plant genomes sequenced, except for one striking feature that went unnoticed at that time: the presence of human-like instead of the expected plant-type telomeric sequences. By using different experimental approaches including fluorescence in situ hybridization (FISH), genome skimming by next-generation sequencing (NGS), and analysis of non-coding transcriptome, we have confirmed its telomeric location in the chromosomes of Z. marina. We have also identified its telomerase RNA (TR) subunit, confirming the presence of the human-type telomeric sequence in the template region. Remarkably, this region was found to be very variable even in clades with a highly conserved telomeric sequence across their species. Based on this observation, we propose that alternative annealing preferences in the template borders can explain the transition between the plant and human telomeric sequences. The further identification of paralogues of TR in several plant genomes led us to the hypothesis that plants may retain an increased ability to change their telomeric sequence. We discuss the implications of this occurrence in the evolution of telomeres while introducing a mechanistic model for the transition from the plant to the human telomeric sequences.

Links

GA19-03442S, research and development project

Name: Geny pro ribozomální RNA - cestovatelé v čase a genomech

Investor: Czech Science Foundation, Ribosomal RNA genes - travellers in time and the genomes

GX20-01331X, research and development project

Name: Biogeneze a evoluce telomerázy u rostlin

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90091, large research infrastructures

Name: NCMG

Citovat

PEŠKA, Vratislav, Martin MÁTL, Terezie MANDÁKOVÁ, Daniel VITALES, Petr FAJKUS, Jiří FAJKUS and Sonia GARCIA. Human-like telomeres in Zostera marina reveal a mode of transition from the plant to the human telomeric sequences. Journal of Experimental Botany. Oxford: Oxford University Press, 2020, vol. 71, No 19, p. 5786-5793. ISSN 0022-0957. Available from: https://dx.doi.org/10.1093/jxb/eraa293.

@article{1703777,
   author = {Peška, Vratislav and Mátl, Martin and Mandáková, Terezie and Vitales, Daniel and Fajkus, Petr and Fajkus, Jiří and Garcia, Sonia},
   article_location = {Oxford},
   article_number = {19},
   doi = {http://dx.doi.org/10.1093/jxb/eraa293},
   keywords = {Animal telomeres; chromosome; cytogenetics; plant telomeres; Tandem Repeats Finder; telomerase RNA; telomere template},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {Human-like telomeres in Zostera marina reveal a mode of transition from the plant to the human telomeric sequences},
   url = {https://doi.org/10.1093/jxb/eraa293},
   volume = {71},
   year = {2020}
}

TY  - JOUR
ID  - 1703777
AU  - Peška, Vratislav - Mátl, Martin - Mandáková, Terezie - Vitales, Daniel - Fajkus, Petr - Fajkus, Jiří - Garcia, Sonia
PY  - 2020
TI  - Human-like telomeres in Zostera marina reveal a mode of transition from the plant to the human telomeric sequences
JF  - Journal of Experimental Botany
VL  - 71
IS  - 19
SP  - 5786-5793
EP  - 5786-5793
PB  - Oxford University Press
SN  - 00220957
KW  - Animal telomeres
KW  - chromosome
KW  - cytogenetics
KW  - plant telomeres
KW  - Tandem Repeats Finder
KW  - telomerase RNA
KW  - telomere template
UR  - https://doi.org/10.1093/jxb/eraa293
L2  - https://doi.org/10.1093/jxb/eraa293
N2  - A previous study describing the genome of Zostera marina, the most widespread seagrass in the Northern hemisphere, revealed some genomic signatures of adaptation to the aquatic environment such as the loss of stomatal genes, while other functions such as an algal-like cell wall composition were acquired. Beyond these, the genome structure and organization were comparable with those of the majority of plant genomes sequenced, except for one striking feature that went unnoticed at that time: the presence of human-like instead of the expected plant-type telomeric sequences. By using different experimental approaches including fluorescence in situ hybridization (FISH), genome skimming by next-generation sequencing (NGS), and analysis of non-coding transcriptome, we have confirmed its telomeric location in the chromosomes of Z. marina. We have also identified its telomerase RNA (TR) subunit, confirming the presence of the human-type telomeric sequence in the template region. Remarkably, this region was found to be very variable even in clades with a highly conserved telomeric sequence across their species. Based on this observation, we propose that alternative annealing preferences in the template borders can explain the transition between the plant and human telomeric sequences. The further identification of paralogues of TR in several plant genomes led us to the hypothesis that plants may retain an increased ability to change their telomeric sequence. We discuss the implications of this occurrence in the evolution of telomeres while introducing a mechanistic model for the transition from the plant to the human telomeric sequences.
ER  -

PEŠKA, Vratislav, Martin MÁTL, Terezie MANDÁKOVÁ, Daniel VITALES, Petr FAJKUS, Jiří FAJKUS and Sonia GARCIA. Human-like telomeres in Zostera marina reveal a mode of transition from the plant to the human telomeric sequences. \textit{Journal of Experimental Botany}. Oxford: Oxford University Press, 2020, vol.~71, No~19, p.~5786-5793. ISSN~0022-0957. Available from: https://dx.doi.org/10.1093/jxb/eraa293.

Detailed Information on Publication Record