Dramatic changes in the electrochemical and spectroscopic
behavior of DNA fragments induced by the loss of a single
nucleotide

J 2020

Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide

TŘÍSKOVÁ, Iveta; Jakub VĚŽNÍK; Karel LACINA a Libuše TRNKOVÁ

Základní údaje

Originální název

Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide

Autoři

TŘÍSKOVÁ, Iveta; Jakub VĚŽNÍK; Karel LACINA a Libuše TRNKOVÁ

Vydání

Bioelectrochemistry, Lausanne, Elsevier, 2020, 1567-5394

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.373

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/20:00117236

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Oligodeoxynucleotide structure; Hairpin; Duplex; GA mismatches; Voltammetry; CD spectra

Štítky

14313010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 12. 2020 08:30, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

In this paper, the d(GCGAAGC) heptamer and the closely related d(GCGAGC) hexamer are examined via electrochemical (cyclic voltammetry) and spectroscopic (circular dichroism) methods. Dramatic changes in the CD spectroscopic and CV electrochemical properties, induced by the loss of only one single nucleotide (A), are detected. The CD spectra and native polyacrylamide gel electrophoresis (PAGE) confirmed structural changes taking place in the relevant chain-like oligodeoxynucleotide assemblies. Dedicated studies suggest that the heptamer (Hp) possesses a hairpin structure, whereas the hexamer (Hx) appears to be rather a duplex. Both of the structures exhibited completely different adsorption behavior at the hanging mercury drop electrode, and this factor was readily confirmed by means of elimination voltammetry with linear scan (EVLS). We established that the Hp hairpin (similar to-1300 mV), compared to the Hx duplex (similar to-1360 mV), is the thermodynamically favored electron acceptor. The adsorption isotherms were constructed based on the voltammetric peak height values, reflecting the reduction of the adenine (A) and cytosine (C) moieties as well as the oxidation of the 7,8-dihydroguanine (7,8-DHG) moieties. Finally, as revealed by the spectroscopic and electrochemical results, Hx forms a bimolecular antiparallel homo-duplex carrying both Watson-Crick base pairs (CG or GC) and mismatched edge-to-edge base pairs (GA or AG). (C) 2020 Published by Elsevier B.V.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

MUNI/A/1359/2018, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2019

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2019, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

TŘÍSKOVÁ, Iveta; Jakub VĚŽNÍK; Karel LACINA a Libuše TRNKOVÁ. Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide. Bioelectrochemistry. Lausanne: Elsevier, 2020, roč. 134, August 2020, s. 1-8. ISSN 1567-5394. Dostupné z: https://doi.org/10.1016/j.bioelechem.2020.107515.

@article{1704220,
   author = {Třísková, Iveta and Věžník, Jakub and Lacina, Karel and Trnková, Libuše},
   article_location = {Lausanne},
   article_number = {August 2020},
   doi = {https://doi.org/10.1016/j.bioelechem.2020.107515},
   keywords = {Oligodeoxynucleotide structure; Hairpin; Duplex; GA mismatches; Voltammetry; CD spectra},
   language = {eng},
   issn = {1567-5394},
   journal = {Bioelectrochemistry},
   note = {Vykazovat pod PřF},
   title = {Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide},
   url = {https://doi.org/10.1016/j.bioelechem.2020.107515},
   volume = {134},
   year = {2020}
}

TY  - JOUR
ID  - 1704220
AU  - Třísková, Iveta - Věžník, Jakub - Lacina, Karel - Trnková, Libuše
PY  - 2020
TI  - Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide
JF  - Bioelectrochemistry
VL  - 134
IS  - August 2020
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 15675394
N1  - Vykazovat pod PřF
KW  - Oligodeoxynucleotide structure
KW  - Hairpin
KW  - Duplex
KW  - GA mismatches
KW  - Voltammetry
KW  - CD spectra
UR  - https://doi.org/10.1016/j.bioelechem.2020.107515
L2  - https://doi.org/10.1016/j.bioelechem.2020.107515
N2  - In this paper, the d(GCGAAGC) heptamer and the closely related d(GCGAGC) hexamer are examined via electrochemical (cyclic voltammetry) and spectroscopic (circular dichroism) methods. Dramatic changes in the CD spectroscopic and CV electrochemical properties, induced by the loss of only one single nucleotide (A), are detected. The CD spectra and native polyacrylamide gel electrophoresis (PAGE) confirmed structural changes taking place in the relevant chain-like oligodeoxynucleotide assemblies. Dedicated studies suggest that the heptamer (Hp) possesses a hairpin structure, whereas the hexamer (Hx) appears to be rather a duplex. Both of the structures exhibited completely different adsorption behavior at the hanging mercury drop electrode, and this factor was readily confirmed by means of elimination voltammetry with linear scan (EVLS). We established that the Hp hairpin (similar to-1300 mV), compared to the Hx duplex (similar to-1360 mV), is the thermodynamically favored electron acceptor. The adsorption isotherms were constructed based on the voltammetric peak height values, reflecting the reduction of the adenine (A) and cytosine (C) moieties as well as the oxidation of the 7,8-dihydroguanine (7,8-DHG) moieties. Finally, as revealed by the spectroscopic and electrochemical results, Hx forms a bimolecular antiparallel homo-duplex carrying both Watson-Crick base pairs (CG or GC) and mismatched edge-to-edge base pairs (GA or AG). (C) 2020 Published by Elsevier B.V.
ER  -

TŘÍSKOVÁ, Iveta; Jakub VĚŽNÍK; Karel LACINA a Libuše TRNKOVÁ. Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide. \textit{Bioelectrochemistry}. Lausanne: Elsevier, 2020, roč.~134, August 2020, s.~1-8. ISSN~1567-5394. Dostupné z: https://doi.org/10.1016/j.bioelechem.2020.107515.

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