Study of alkaloid berberine and its interaction with the human
telomeric i-motif DNA structure

J 2021

Study of alkaloid berberine and its interaction with the human telomeric i-motif DNA structure

GARGALLO, Raimundo, Anna AVIÑÓ, Ramon ERITJA, Petra JAROŠOVÁ, Stefania MAZZINI et. al.

Základní údaje

Originální název

Study of alkaloid berberine and its interaction with the human telomeric i-motif DNA structure

Název česky

Alkaloid berberin a jeho interakce s lidskou telomerní DNA strukturou i-motiv

Autoři

GARGALLO, Raimundo (724 Španělsko), Anna AVIÑÓ (724 Španělsko), Ramon ERITJA, Petra JAROŠOVÁ (203 Česká republika, domácí), Stefania MAZZINI, Leonardo SCAGLIONI a Petr TÁBORSKÝ (203 Česká republika, garant, domácí)

Vydání

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Oxford, Elsevier, 2021, 1386-1425

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.831

Kód RIV

RIV/00216224:14310/21:00120864

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.saa.2020.119185

UT WoS

000608169300014

Klíčová slova česky

Berberin; i-motiv; G-kvaduplex

Klíčová slova anglicky

Berberine; I-motif; DNA binders; G-quadruplex

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 2. 2021 14:02, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The alkaloid berberine presents many biological activities related to its potential to bind DNA structures, such as duplex or G-quadruplex. Recently, it has been proposed that berberine may interact with i-motif structures formed from the folding of cytosine-rich sequences. In the present work, the interaction of this alkaloid with the i-motif formed by the human telomere cytosine-rich sequence, as well as with several positive and negative controls, has been studied. Molecular fluorescence and circular dichroism spectroscopies, as well as nuclear magnetic resonance spectrometry and competitive dialysis, have been used with this purpose. The results shown here reveal that the interaction of berberine with this i-motif is weak, mostly electrostatics in nature and takes place with bases not involved in C·C+ base pairs. Moreover, this ligand is not selective for i-motif structures, as binds equally to both, folded structure, and unfolded strand, without producing any stabilization of the i-motif. As a conclusion, the development of analytical methods based on the interaction of fluorescent ligands, such as berberine, with i-motif structures should consider the thermodynamic aspects related with the interaction, as well as the selectivity of the proposed ligands with different DNA structures, including unfolded strands.

Citovat

GARGALLO, Raimundo, Anna AVIÑÓ, Ramon ERITJA, Petra JAROŠOVÁ, Stefania MAZZINI, Leonardo SCAGLIONI a Petr TÁBORSKÝ. Study of alkaloid berberine and its interaction with the human telomeric i-motif DNA structure. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. Oxford: Elsevier, 2021, roč. 248, March 2021, s. 1-12. ISSN 1386-1425. Dostupné z: https://dx.doi.org/10.1016/j.saa.2020.119185.

@article{1707637,
   author = {Gargallo, Raimundo and Aviñó, Anna and Eritja, Ramon and Jarošová, Petra and Mazzini, Stefania and Scaglioni, Leonardo and Táborský, Petr},
   article_location = {Oxford},
   article_number = {March 2021},
   doi = {http://dx.doi.org/10.1016/j.saa.2020.119185},
   keywords = {Berberine; I-motif; DNA binders; G-quadruplex},
   language = {eng},
   issn = {1386-1425},
   journal = {Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy},
   title = {Study of alkaloid berberine and its interaction with the human telomeric i-motif DNA structure},
   url = {https://www.sciencedirect.com/science/article/abs/pii/S1386142520311641},
   volume = {248},
   year = {2021}
}

TY  - JOUR
ID  - 1707637
AU  - Gargallo, Raimundo - Aviñó, Anna - Eritja, Ramon - Jarošová, Petra - Mazzini, Stefania - Scaglioni, Leonardo - Táborský, Petr
PY  - 2021
TI  - Study of alkaloid berberine and its interaction with the human telomeric i-motif DNA structure
JF  - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
VL  - 248
IS  - March 2021
SP  - 1-12
EP  - 1-12
PB  - Elsevier
SN  - 13861425
KW  - Berberine
KW  - I-motif
KW  - DNA binders
KW  - G-quadruplex
UR  - https://www.sciencedirect.com/science/article/abs/pii/S1386142520311641
L2  - https://www.sciencedirect.com/science/article/abs/pii/S1386142520311641
N2  - The alkaloid berberine presents many biological activities related to its potential to bind DNA structures, such as duplex or G-quadruplex. Recently, it has been proposed that berberine may interact with i-motif structures formed from the folding of cytosine-rich sequences. In the present work, the interaction of this alkaloid with the i-motif formed by the human telomere cytosine-rich sequence, as well as with several positive and negative controls, has been studied. Molecular fluorescence and circular dichroism spectroscopies, as well as nuclear magnetic resonance spectrometry and competitive dialysis, have been used with this purpose. The results shown here reveal that the interaction of berberine with this i-motif is weak, mostly electrostatics in nature and takes place with bases not involved in C·C+ base pairs. Moreover, this ligand is not selective for i-motif structures, as binds equally to both, folded structure, and unfolded strand, without producing any stabilization of the i-motif. As a conclusion, the development of analytical methods based on the interaction of fluorescent ligands, such as berberine, with i-motif structures should consider the thermodynamic aspects related with the interaction, as well as the selectivity of the proposed ligands with different DNA structures, including unfolded strands.
ER  -

GARGALLO, Raimundo, Anna AVIÑÓ, Ramon ERITJA, Petra JAROŠOVÁ, Stefania MAZZINI, Leonardo SCAGLIONI a Petr TÁBORSKÝ. Study of alkaloid berberine and its interaction with the human telomeric i-motif DNA structure. \textit{Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy}. Oxford: Elsevier, 2021, roč.~248, March 2021, s.~1-12. ISSN~1386-1425. Dostupné z: https://dx.doi.org/10.1016/j.saa.2020.119185.

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