Structure-Dependent Effects of Phthalates on Intercellular and
Intracellular Communication in Liver Oval Cells

J 2020

Structure-Dependent Effects of Phthalates on Intercellular and Intracellular Communication in Liver Oval Cells

ČTVERÁČKOVÁ, Lucie; Daniel JANČULA; Jan RAŠKA; Pavel BABICA; Iva SOVADINOVÁ et al.

Základní údaje

Originální název

Structure-Dependent Effects of Phthalates on Intercellular and Intracellular Communication in Liver Oval Cells

Autoři

ČTVERÁČKOVÁ, Lucie; Daniel JANČULA; Jan RAŠKA; Pavel BABICA a Iva SOVADINOVÁ

Vydání

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2020, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/20:00114568

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

gap junctional intercellular communication; gap junctions; hepatotoxicity; MAP-kinases Erk1; 2 activation; non-genomic mechanism; oval cells; phthalates; progenitor cells

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 6. 2025 21:50, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Humans are exposed to phthalates released from plastics, cosmetics, or food on a daily basis. Phthalates have low acute liver toxicity, but their chronic exposures could induce molecular and cellular effects linked to adverse health outcomes, such as liver tumor promotion or chronic liver diseases. The alternation of gap junctional intercellular communication (GJIC) and MAPK-Erk1/2 pathways in liver progenitor or oval cells can disrupt liver tissue homeostatic mechanisms and affect the development and severity of these adverse outcomes. Our study with 20 different phthalates revealed their structurally dependent effects on liver GJIC and MAPK-Erk1/2 signaling in rat liver WB-F344 cell line with characteristics of liver oval cells. The phthalates with a medium-length side chain (3-6 C) were the most potent dysregulators of GJIC and activators of MAPK-Erk1/2. The effects occurred rapidly, suggesting the activation of non-genomic (non-transcriptional) mechanisms directly by the parental compounds. Short-chain phthalates (1-2 C) did not dysregulate GJIC even after longer exposures and did not activate MAPK-Erk1/2. Longer chain (>= 7 C) phthalates, such as DEHP or DINP, moderately activated MAPK-Erk1/2, but inhibited GJIC only after prolonged exposures (>12 h), suggesting that GJIC dysregulation occurs via genomic mechanisms, or (bio)transformation. Overall, medium-chain phthalates rapidly affected the key tissue homeostatic mechanisms in the liver oval cell population via non-genomic pathways, which might contribute to the development of chronic liver toxicity and diseases.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

GJ16-10775Y, projekt VaV

Název: Mezibuněčná komunikace zprostředkovaná mezerovými spoji jako cíl endokrinních disruptorů v testikulárních buňkách

Investor: Grantová agentura ČR, Mezibuněčná komunikace zprostředkovaná mezerovými spoji jako cíl endokrinních disruptorů v testikulárních buňkách

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

90121, velká výzkumná infrastruktura

Název: RECETOX RI

Přiložené soubory

1708576.pdf

Citovat

ČTVERÁČKOVÁ, Lucie; Daniel JANČULA; Jan RAŠKA; Pavel BABICA a Iva SOVADINOVÁ. Structure-Dependent Effects of Phthalates on Intercellular and Intracellular Communication in Liver Oval Cells. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2020, roč. 21, č. 17, s. 1-21. ISSN 1422-0067. Dostupné z: https://doi.org/10.3390/ijms21176069.

@article{1708576,
   author = {Čtveráčková, Lucie and Jančula, Daniel and Raška, Jan and Babica, Pavel and Sovadinová, Iva},
   article_location = {Basel},
   article_number = {17},
   doi = {https://doi.org/10.3390/ijms21176069},
   keywords = {gap junctional intercellular communication; gap junctions; hepatotoxicity; MAP-kinases Erk1; 2 activation; non-genomic mechanism; oval cells; phthalates; progenitor cells},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   note = {CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat.},
   title = {Structure-Dependent Effects of Phthalates on Intercellular and Intracellular Communication in Liver Oval Cells},
   url = {https://www.mdpi.com/1422-0067/21/17/6069},
   volume = {21},
   year = {2020}
}

TY  - JOUR
ID  - 1708576
AU  - Čtveráčková, Lucie - Jančula, Daniel - Raška, Jan - Babica, Pavel - Sovadinová, Iva
PY  - 2020
TI  - Structure-Dependent Effects of Phthalates on Intercellular and Intracellular Communication in Liver Oval Cells
JF  - International Journal of Molecular Sciences
VL  - 21
IS  - 17
SP  - 1-21
EP  - 1-21
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
N1  - CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat.
KW  - gap junctional intercellular communication
KW  - gap junctions
KW  - hepatotoxicity
KW  - MAP-kinases Erk1
KW  - 2 activation
KW  - non-genomic mechanism
KW  - oval cells
KW  - phthalates
KW  - progenitor cells
UR  - https://www.mdpi.com/1422-0067/21/17/6069
L2  - https://www.mdpi.com/1422-0067/21/17/6069
N2  - Humans are exposed to phthalates released from plastics, cosmetics, or food on a daily basis. Phthalates have low acute liver toxicity, but their chronic exposures could induce molecular and cellular effects linked to adverse health outcomes, such as liver tumor promotion or chronic liver diseases. The alternation of gap junctional intercellular communication (GJIC) and MAPK-Erk1/2 pathways in liver progenitor or oval cells can disrupt liver tissue homeostatic mechanisms and affect the development and severity of these adverse outcomes. Our study with 20 different phthalates revealed their structurally dependent effects on liver GJIC and MAPK-Erk1/2 signaling in rat liver WB-F344 cell line with characteristics of liver oval cells. The phthalates with a medium-length side chain (3-6 C) were the most potent dysregulators of GJIC and activators of MAPK-Erk1/2. The effects occurred rapidly, suggesting the activation of non-genomic (non-transcriptional) mechanisms directly by the parental compounds. Short-chain phthalates (1-2 C) did not dysregulate GJIC even after longer exposures and did not activate MAPK-Erk1/2. Longer chain (>= 7 C) phthalates, such as DEHP or DINP, moderately activated MAPK-Erk1/2, but inhibited GJIC only after prolonged exposures (>12 h), suggesting that GJIC dysregulation occurs via genomic mechanisms, or (bio)transformation. Overall, medium-chain phthalates rapidly affected the key tissue homeostatic mechanisms in the liver oval cell population via non-genomic pathways, which might contribute to the development of chronic liver toxicity and diseases.
ER  -

ČTVERÁČKOVÁ, Lucie; Daniel JANČULA; Jan RAŠKA; Pavel BABICA a Iva SOVADINOVÁ. Structure-Dependent Effects of Phthalates on Intercellular and Intracellular Communication in Liver Oval Cells. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2020, roč.~21, č.~17, s.~1-21. ISSN~1422-0067. Dostupné z: https://doi.org/10.3390/ijms21176069.

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