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@article{1710056, author = {Veselý, Lukáš and Behera, Susrisweta and Heger, Dominik}, article_location = {Amsterdam}, article_number = {January 2021}, doi = {http://dx.doi.org/10.1016/j.ijpharm.2020.120128}, keywords = {pH shift; Cryopreservation; Stabilization; Freeze-concentrated solution; Sulfonephthalein indicators; Hammett acidity function; Cooling rate}, language = {eng}, issn = {0378-5173}, journal = {International Journal of Pharmaceutics}, title = {Making good’s buffers good for freezing: The acidity changes and their elimination via mixing with sodium phosphate}, url = {https://doi.org/10.1016/j.ijpharm.2020.120128}, volume = {593}, year = {2021} }
TY - JOUR ID - 1710056 AU - Veselý, Lukáš - Behera, Susrisweta - Heger, Dominik PY - 2021 TI - Making good’s buffers good for freezing: The acidity changes and their elimination via mixing with sodium phosphate JF - International Journal of Pharmaceutics VL - 593 IS - January 2021 SP - 1-9 EP - 1-9 PB - Elsevier SN - 03785173 KW - pH shift KW - Cryopreservation KW - Stabilization KW - Freeze-concentrated solution KW - Sulfonephthalein indicators KW - Hammett acidity function KW - Cooling rate UR - https://doi.org/10.1016/j.ijpharm.2020.120128 L2 - https://doi.org/10.1016/j.ijpharm.2020.120128 N2 - Solutions of three Good’s buffers (HEPES, MOPS, and MES), both pure and mixed with sodium phosphate buffers (Na-P), are investigated in terms of the freezing-induced acidity changes in their operational pH ranges. The Good’s buffers have the tendency to basify upon freezing and, more intensively, at lower pHs. The acidity varies most prominently in MES, where the change may reach the value of two. Importantly, the Good’s buffers are shown to mitigate the strong acidification in the Na-P buffer. Diverse concentrations of the Good’s buffers are added to cancel out the strong, freezing-induced acidity drop in 50 mM Na-P that markedly contributes to the solution’s acidity; the relevant values are 3 mM HEPES, 10 mM MOPS, and 80 mM MES. These buffer blends are therefore proposed to be applied in maintaining approximately the acidity of solutions even after the freezing process and, as such, should limit the stresses for frozen chemicals and biochemicals. ER -
VESELÝ, Lukáš, Susrisweta BEHERA a Dominik HEGER. Making good’s buffers good for freezing: The acidity changes and their elimination via mixing with sodium phosphate. \textit{International Journal of Pharmaceutics}. Amsterdam: Elsevier, 2021, roč.~593, January 2021, s.~1-9. ISSN~0378-5173. Dostupné z: https://dx.doi.org/10.1016/j.ijpharm.2020.120128.
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