Fundamentally different repetitive element composition of sex
chromosomes in Rumex acetosa

J 2021

Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa

JESIONEK, Wojciech Krzysztof; Markéta BODLÁKOVÁ; Zdeněk KUBÁT; Radim ČEGAN; Boris VYSKOT et al.

Základní údaje

Originální název

Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa

Autoři

JESIONEK, Wojciech Krzysztof ; Markéta BODLÁKOVÁ; Zdeněk KUBÁT; Radim ČEGAN; Boris VYSKOT; Jan VRÁNA; Jan ŠAFÁŘ; Janka PUTEROVA a Roman HOBZA

Vydání

Annals of Botany, Oxford, Oxford University Press, 2021, 0305-7364

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.040

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00120869

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Rumex acetosa; sex chromosomes; genome dynamics; transposable elements; satellites

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 6. 2021 09:52, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Abstract Background and Aims Dioecious species with well-established sex chromosomes are rare in the plant kingdom. Most sex chromosomes increase in size but no comprehensive analysis of the kind of sequences that drive this expansion has been presented. Here we analyse sex chromosome structure in common sorrel (Rumex acetosa), a dioecious plant with XY1Y2 sex determination, and we provide the first chromosome-specific repeatome analysis for a plant species possessing sex chromosomes. Methods We flow-sorted and separately sequenced sex chromosomes and autosomes in R. acetosa using the two-dimensional fluorescence in situ hybridization in suspension (FISHIS) method and Illumina sequencing. We identified and quantified individual repeats using RepeatExplorer, Tandem Repeat Finder and the Tandem Repeats Analysis Program. We employed fluorescence in situ hybridization (FISH) to analyse the chromosomal localization of satellites and transposons. Key Results We identified a number of novel satellites, which have, in a fashion similar to previously known satellites, significantly expanded on the Y chromosome but not as much on the X or on autosomes. Additionally, the size increase of Y chromosomes is caused by non-long terminal repeat (LTR) and LTR retrotransposons, while only the latter contribute to the enlargement of the X chromosome. However, the X chromosome is populated by different LTR retrotransposon lineages than those on Y chromosomes. Conclusions The X and Y chromosomes have significantly diverged in terms of repeat composition. The lack of recombination probably contributed to the expansion of diverse satellites and microsatellites and faster fixation of newly inserted transposable elements (TEs) on the Y chromosomes. In addition, the X and Y chromosomes, despite similar total counts of TEs, differ significantly in the representation of individual TE lineages, which indicates that transposons proliferate preferentially in either the paternal or the maternal lineage.

Citovat

JESIONEK, Wojciech Krzysztof; Markéta BODLÁKOVÁ; Zdeněk KUBÁT; Radim ČEGAN; Boris VYSKOT; Jan VRÁNA; Jan ŠAFÁŘ; Janka PUTEROVA a Roman HOBZA. Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa. Annals of Botany. Oxford: Oxford University Press, 2021, roč. 127, č. 1, s. 33-47. ISSN 0305-7364. Dostupné z: https://doi.org/10.1093/aob/mcaa160.

@article{1710216,
   author = {Jesionek, Wojciech Krzysztof and Bodláková, Markéta and Kubát, Zdeněk and Čegan, Radim and Vyskot, Boris and Vrána, Jan and Šafář, Jan and Puterova, Janka and Hobza, Roman},
   article_location = {Oxford},
   article_number = {1},
   doi = {https://doi.org/10.1093/aob/mcaa160},
   keywords = {Rumex acetosa; sex chromosomes; genome dynamics; transposable elements; satellites},
   language = {eng},
   issn = {0305-7364},
   journal = {Annals of Botany},
   title = {Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa},
   url = {https://doi.org/10.1093/aob/mcaa160},
   volume = {127},
   year = {2021}
}

TY  - JOUR
ID  - 1710216
AU  - Jesionek, Wojciech Krzysztof - Bodláková, Markéta - Kubát, Zdeněk - Čegan, Radim - Vyskot, Boris - Vrána, Jan - Šafář, Jan - Puterova, Janka - Hobza, Roman
PY  - 2021
TI  - Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa
JF  - Annals of Botany
VL  - 127
IS  - 1
SP  - 33-47
EP  - 33-47
PB  - Oxford University Press
SN  - 03057364
KW  - Rumex acetosa
KW  - sex chromosomes
KW  - genome dynamics
KW  - transposable elements
KW  - satellites
UR  - https://doi.org/10.1093/aob/mcaa160
L2  - https://doi.org/10.1093/aob/mcaa160
N2  - Abstract Background and Aims Dioecious species with well-established sex chromosomes are rare in the plant kingdom. Most sex chromosomes increase in size but no comprehensive analysis of the kind of sequences that drive this expansion has been presented. Here we analyse sex chromosome structure in common sorrel (Rumex acetosa), a dioecious plant with XY1Y2 sex determination, and we provide the first chromosome-specific repeatome analysis for a plant species possessing sex chromosomes. Methods We flow-sorted and separately sequenced sex chromosomes and autosomes in R. acetosa using the two-dimensional fluorescence in situ hybridization in suspension (FISHIS) method and Illumina sequencing. We identified and quantified individual repeats using RepeatExplorer, Tandem Repeat Finder and the Tandem Repeats Analysis Program. We employed fluorescence in situ hybridization (FISH) to analyse the chromosomal localization of satellites and transposons. Key Results We identified a number of novel satellites, which have, in a fashion similar to previously known satellites, significantly expanded on the Y chromosome but not as much on the X or on autosomes. Additionally, the size increase of Y chromosomes is caused by non-long terminal repeat (LTR) and LTR retrotransposons, while only the latter contribute to the enlargement of the X chromosome. However, the X chromosome is populated by different LTR retrotransposon lineages than those on Y chromosomes. Conclusions The X and Y chromosomes have significantly diverged in terms of repeat composition. The lack of recombination probably contributed to the expansion of diverse satellites and microsatellites and faster fixation of newly inserted transposable elements (TEs) on the Y chromosomes. In addition, the X and Y chromosomes, despite similar total counts of TEs, differ significantly in the representation of individual TE lineages, which indicates that transposons proliferate preferentially in either the paternal or the maternal lineage.
ER  -

JESIONEK, Wojciech Krzysztof; Markéta BODLÁKOVÁ; Zdeněk KUBÁT; Radim ČEGAN; Boris VYSKOT; Jan VRÁNA; Jan ŠAFÁŘ; Janka PUTEROVA a Roman HOBZA. Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa. \textit{Annals of Botany}. Oxford: Oxford University Press, 2021, roč.~127, č.~1, s.~33-47. ISSN~0305-7364. Dostupné z: https://doi.org/10.1093/aob/mcaa160.

Přehled o publikaci