Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus
sp. nov., isolated from regoliths in Antarctica

J 2020

Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica

SEDLÁČEK, Ivo, Roman PANTŮČEK, Michal ZEMAN, Pavla HOLOCHOVÁ, Ondrej ŠEDO et. al.

Základní údaje

Originální název

Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica

Autoři

SEDLÁČEK, Ivo (203 Česká republika, garant, domácí), Roman PANTŮČEK (203 Česká republika, domácí), Michal ZEMAN (703 Slovensko, domácí), Pavla HOLOCHOVÁ (203 Česká republika, domácí), Ondrej ŠEDO (203 Česká republika, domácí), Eva STAŇKOVÁ (203 Česká republika, domácí), Pavel ŠVEC (203 Česká republika, domácí), Stanislava KRÁLOVÁ (703 Slovensko, domácí), Petra VÍDEŇSKÁ (203 Česká republika, domácí), Lenka MICENKOVÁ (703 Slovensko, domácí), Sah URVASHI (356 Indie), Suresh KORPOLE (356 Indie) a Rup LAL (356 Indie)

Vydání

International Journal of Systematic and Evolutionary Microbiology, London, Microbiology Society, 2020, 1466-5026

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.747

Kód RIV

RIV/00216224:14310/20:00117439

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1099/ijsem.0.004540

UT WoS

000608418900041

Klíčová slova anglicky

Hymenobacter; regoliths; taxonomy; Antarctica

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 20:29, Ing. Martina Blahová

Anotace

V originále

A group of four psychrotrophic bacterial strains was isolated on James Ross Island (Antarctica) in 2013. All isolates, originating from different soil samples, were collected from the ice-free northern part of the island. They were rod-shaped, Gram-stain-negative, and produced moderately slimy red-pink pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, MALDI-TOF MS, rep-PCR analyses, chemotaxonomic methods and extensive biotyping was used to clarify the taxonomic position of these isolates. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates belonged to the genus Hymenobacter. The closest relative was Hymenobacter humicola CCM 8763T, exhibiting 98.3 and 98.9% 16S rRNA pairwise similarity with the reference isolates P5342T and P5252T, respectively. Average nucleotide identity, digital DNA–DNA hybridization and core gene distances calculated from the whole-genome sequencing data confirmed that P5252T and P5342T represent two distinct Hymenobacter species. The menaquinone systems of both strains contained MK-7 as the major respiratory quinone. The predominant polar lipids for both strains were phosphatidylethanolamine and one unidentified glycolipid. The major components in the cellular fatty acid composition were summed feature 3, summed feature 4 (anteiso-C17:1 B/iso-C17:1 I), anteiso-C15:0 and iso-C15 : 0 for all isolates. Based on the obtained results, two novel species are proposed, for which the names Hymenobacter terrestris sp. nov. (type strain P5252T=CCM 8765T=LMG 31495T) and Hymenobacter lapidiphilus sp. nov. (type strain P5342T=CCM 8764T=LMG 30613T) are suggested.

Návaznosti

EF16_013/0001761, projekt VaV

Název: RECETOX RI

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

LM2015078, projekt VaV

Název: Česká polární výzkumná infrastruktura (Akronym: CzechPolar2)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar Research Infrastructure

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

MUNI/A/1127/2019, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 8 (Akronym: MBG 8)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 8, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

SEDLÁČEK, Ivo, Roman PANTŮČEK, Michal ZEMAN, Pavla HOLOCHOVÁ, Ondrej ŠEDO, Eva STAŇKOVÁ, Pavel ŠVEC, Stanislava KRÁLOVÁ, Petra VÍDEŇSKÁ, Lenka MICENKOVÁ, Sah URVASHI, Suresh KORPOLE a Rup LAL. Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica. International Journal of Systematic and Evolutionary Microbiology. London: Microbiology Society, 2020, roč. 70, č. 12, s. 6364-6372. ISSN 1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijsem.0.004540.

@article{1712336,
   author = {Sedláček, Ivo and Pantůček, Roman and Zeman, Michal and Holochová, Pavla and Šedo, Ondrej and Staňková, Eva and Švec, Pavel and Králová, Stanislava and Vídeňská, Petra and Micenková, Lenka and Urvashi, Sah and Korpole, Suresh and Lal, Rup},
   article_location = {London},
   article_number = {12},
   doi = {http://dx.doi.org/10.1099/ijsem.0.004540},
   keywords = {Hymenobacter; regoliths; taxonomy; Antarctica},
   language = {eng},
   issn = {1466-5026},
   journal = {International Journal of Systematic and Evolutionary Microbiology},
   note = {CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat. Místo toho uplatnit EF17_043/0009632.},
   title = {Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica},
   url = {https://doi.org/10.1099/ijsem.0.004540},
   volume = {70},
   year = {2020}
}

TY  - JOUR
ID  - 1712336
AU  - Sedláček, Ivo - Pantůček, Roman - Zeman, Michal - Holochová, Pavla - Šedo, Ondrej - Staňková, Eva - Švec, Pavel - Králová, Stanislava - Vídeňská, Petra - Micenková, Lenka - Urvashi, Sah - Korpole, Suresh - Lal, Rup
PY  - 2020
TI  - Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica
JF  - International Journal of Systematic and Evolutionary Microbiology
VL  - 70
IS  - 12
SP  - 6364-6372
EP  - 6364-6372
PB  - Microbiology Society
SN  - 14665026
N1  - CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat. Místo toho uplatnit EF17_043/0009632.
KW  - Hymenobacter
KW  - regoliths
KW  - taxonomy
KW  - Antarctica
UR  - https://doi.org/10.1099/ijsem.0.004540
L2  - https://doi.org/10.1099/ijsem.0.004540
N2  - A group of four psychrotrophic bacterial strains was isolated on James Ross Island (Antarctica) in 2013. All isolates, originating from different soil samples, were collected from the ice-free northern part of the island. They were rod-shaped, Gram-stain-negative, and produced moderately slimy red-pink pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, MALDI-TOF MS, rep-PCR analyses, chemotaxonomic methods and extensive biotyping was used to clarify the taxonomic position of these isolates. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates belonged to the genus Hymenobacter. The closest relative was Hymenobacter humicola CCM 8763T, exhibiting 98.3 and 98.9% 16S rRNA pairwise similarity with the reference isolates P5342T and P5252T, respectively. Average nucleotide identity, digital DNA–DNA hybridization and core gene distances calculated from the whole-genome sequencing data confirmed that P5252T and P5342T represent two distinct Hymenobacter species. The menaquinone systems of both strains contained MK-7 as the major respiratory quinone. The predominant polar lipids for both strains were phosphatidylethanolamine and one unidentified glycolipid. The major components in the cellular fatty acid composition were summed feature 3, summed feature 4 (anteiso-C17:1 B/iso-C17:1 I), anteiso-C15:0 and iso-C15 : 0 for all isolates. Based on the obtained results, two novel species are proposed, for which the names Hymenobacter terrestris sp. nov. (type strain P5252T=CCM 8765T=LMG 31495T) and Hymenobacter lapidiphilus sp. nov. (type strain P5342T=CCM 8764T=LMG 30613T) are suggested.
ER  -

SEDLÁČEK, Ivo, Roman PANTŮČEK, Michal ZEMAN, Pavla HOLOCHOVÁ, Ondrej ŠEDO, Eva STAŇKOVÁ, Pavel ŠVEC, Stanislava KRÁLOVÁ, Petra VÍDEŇSKÁ, Lenka MICENKOVÁ, Sah URVASHI, Suresh KORPOLE a Rup LAL. Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica. \textit{International Journal of Systematic and Evolutionary Microbiology}. London: Microbiology Society, 2020, roč.~70, č.~12, s.~6364-6372. ISSN~1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijsem.0.004540.

Podrobný výpis o publikaci