Neurobehavioral effects of cyanobacterial biomass field
extracts on zebrafish embryos and potential role of retinoids

J 2020

Neurobehavioral effects of cyanobacterial biomass field extracts on zebrafish embryos and potential role of retinoids

PÍPAL, Marek; Jessica LEGRADI; Marie SMUTNÁ; Tereza KOCI; Jana PRIEBOJOVÁ et al.

Základní údaje

Originální název

Neurobehavioral effects of cyanobacterial biomass field extracts on zebrafish embryos and potential role of retinoids

Autoři

PÍPAL, Marek; Jessica LEGRADI; Marie SMUTNÁ; Tereza KOCI; Jana PRIEBOJOVÁ; Lucie BLÁHOVÁ; Martin KRAUSS a Klára HILSCHEROVÁ

Vydání

Aquatic toxicology, Amsterdam, Elsevier Science BV, 2020, 0166-445X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30108 Toxicology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.964

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/20:00114601

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Cyanobacteria; Locomotor response; Zebrafish; Retinoids; ATRA; Biomarkers

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 6. 2025 09:25, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Cyanobacteria are known for their ability to produce and release mixtures of up to thousands of compounds into the environment. Recently, the production of novel metabolites, retinoids, was reported for some cyanobacterial species along with teratogenic effects of samples containing these compounds. Retinoids are natural endogenous substances derived from vitamin A that play a crucial role in early vertebrate development. Disruption of retinoid signalling-especially during the early development of the nervous system- might lead to major malfunctions and malformations. In this study, the toxicity of cyanobacterial biomass samples from the field containing retinoids was characterized by in vivo and in vitro bioassays with a focus on the potential hazards towards nervous system development and function. Additionally, in order to identify the compounds responsible for the observed in vitro and in vivo effects the complex cyanobacterial extracts were fractionated (C18 column, water-methanol gradient) and the twelve obtained fractions were tested in bioassays. In all bioassays, all-trans retinoic acid (ATRA) was tested along with the environmental samples as a positive control. Retinoid-like activity (mediated via the retinoic acid receptor, RAR) was measured in the transgenic cell line p19/A15. The in vitro assay showed retinoid-like activity by specific interaction with RAR for the biomass samples. Neurotoxic effects of selected samples were studied on zebrafish (Danio rerio) embryos using the light/dark transition test (Viewpoint, ZebraLab system) with 120 hpf larvae. In the behavioural assay, the cyanobacterial extracts caused significant hyperactivity in zebrafish at 120 hpf after acute exposure (3 h prior to the measurement) at concentrations below the teratogenicity LOEC (0.2 g dw L-1). Similar effect was observed after exposure to fractions of the extracts with detected retinoid-like activity and additive effect was observed after combining the fractions. However, the effect on behaviour was not observed after exposure to ATRA only. To provide additional insight into the behavioural effects and describe the underlying mechanism gene expression of selected biomarkers was measured. We evaluated an array of 28 genes related to general toxicity, neurodevelopment, retinoid and thyroid signalling. We detected several affected genes, most notably, the Cyp26 enzymes that control endogenous ATRA concentration, which documents an effect on retinoid signalling.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

GA18-15199S, projekt VaV

Název: Bioaktivní látky ze sinic ovlivňující signálování jaderných receptorů a vývoj obratlovců

Investor: Grantová agentura ČR, Bioaktivní látky ze sinic ovlivňující signálování jaderných receptorů a vývoj obratlovců

90121, velká výzkumná infrastruktura

Název: RECETOX RI

Citovat

PÍPAL, Marek; Jessica LEGRADI; Marie SMUTNÁ; Tereza KOCI; Jana PRIEBOJOVÁ; Lucie BLÁHOVÁ; Martin KRAUSS a Klára HILSCHEROVÁ. Neurobehavioral effects of cyanobacterial biomass field extracts on zebrafish embryos and potential role of retinoids. Aquatic toxicology. Amsterdam: Elsevier Science BV, 2020, roč. 228, November 2020, s. 1-12. ISSN 0166-445X. Dostupné z: https://doi.org/10.1016/j.aquatox.2020.105613.

@article{1715398,
   author = {Pípal, Marek and Legradi, Jessica and Smutná, Marie and Koci, Tereza and Priebojová, Jana and Bláhová, Lucie and Krauss, Martin and Hilscherová, Klára},
   article_location = {Amsterdam},
   article_number = {November 2020},
   doi = {https://doi.org/10.1016/j.aquatox.2020.105613},
   keywords = {Cyanobacteria; Locomotor response; Zebrafish; Retinoids; ATRA; Biomarkers},
   language = {eng},
   issn = {0166-445X},
   journal = {Aquatic toxicology},
   note = {CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat.},
   title = {Neurobehavioral effects of cyanobacterial biomass field extracts on zebrafish embryos and potential role of retinoids},
   url = {https://www.sciencedirect.com/science/article/pii/S0166445X20303635?via%3Dihub},
   volume = {228},
   year = {2020}
}

TY  - JOUR
ID  - 1715398
AU  - Pípal, Marek - Legradi, Jessica - Smutná, Marie - Koci, Tereza - Priebojová, Jana - Bláhová, Lucie - Krauss, Martin - Hilscherová, Klára
PY  - 2020
TI  - Neurobehavioral effects of cyanobacterial biomass field extracts on zebrafish embryos and potential role of retinoids
JF  - Aquatic toxicology
VL  - 228
IS  - November 2020
SP  - 1-12
EP  - 1-12
PB  - Elsevier Science BV
SN  - 0166445X
N1  - CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat.
KW  - Cyanobacteria
KW  - Locomotor response
KW  - Zebrafish
KW  - Retinoids
KW  - ATRA
KW  - Biomarkers
UR  - https://www.sciencedirect.com/science/article/pii/S0166445X20303635?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0166445X20303635?via%3Dihub
N2  - Cyanobacteria are known for their ability to produce and release mixtures of up to thousands of compounds into the environment. Recently, the production of novel metabolites, retinoids, was reported for some cyanobacterial species along with teratogenic effects of samples containing these compounds. Retinoids are natural endogenous substances derived from vitamin A that play a crucial role in early vertebrate development. Disruption of retinoid signalling-especially during the early development of the nervous system- might lead to major malfunctions and malformations. In this study, the toxicity of cyanobacterial biomass samples from the field containing retinoids was characterized by in vivo and in vitro bioassays with a focus on the potential hazards towards nervous system development and function. Additionally, in order to identify the compounds responsible for the observed in vitro and in vivo effects the complex cyanobacterial extracts were fractionated (C18 column, water-methanol gradient) and the twelve obtained fractions were tested in bioassays. In all bioassays, all-trans retinoic acid (ATRA) was tested along with the environmental samples as a positive control. Retinoid-like activity (mediated via the retinoic acid receptor, RAR) was measured in the transgenic cell line p19/A15. The in vitro assay showed retinoid-like activity by specific interaction with RAR for the biomass samples. Neurotoxic effects of selected samples were studied on zebrafish (Danio rerio) embryos using the light/dark transition test (Viewpoint, ZebraLab system) with 120 hpf larvae. In the behavioural assay, the cyanobacterial extracts caused significant hyperactivity in zebrafish at 120 hpf after acute exposure (3 h prior to the measurement) at concentrations below the teratogenicity LOEC (0.2 g dw L-1). Similar effect was observed after exposure to fractions of the extracts with detected retinoid-like activity and additive effect was observed after combining the fractions. However, the effect on behaviour was not observed after exposure to ATRA only. To provide additional insight into the behavioural effects and describe the underlying mechanism gene expression of selected biomarkers was measured. We evaluated an array of 28 genes related to general toxicity, neurodevelopment, retinoid and thyroid signalling. We detected several affected genes, most notably, the Cyp26 enzymes that control endogenous ATRA concentration, which documents an effect on retinoid signalling.
ER  -

PÍPAL, Marek; Jessica LEGRADI; Marie SMUTNÁ; Tereza KOCI; Jana PRIEBOJOVÁ; Lucie BLÁHOVÁ; Martin KRAUSS a Klára HILSCHEROVÁ. Neurobehavioral effects of cyanobacterial biomass field extracts on zebrafish embryos and potential role of retinoids. \textit{Aquatic toxicology}. Amsterdam: Elsevier Science BV, 2020, roč.~228, November 2020, s.~1-12. ISSN~0166-445X. Dostupné z: https://doi.org/10.1016/j.aquatox.2020.105613.

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