N-Formylated Peptide Induces Increased Expression of Both
Formyl Peptide Receptor 2 (Fpr2) and Toll-Like Receptor 9
(TLR9) in Schwannoma Cells-An In Vitro Model for Early
Inflammatory Profiling of Schwann Cells

J 2020

N-Formylated Peptide Induces Increased Expression of Both Formyl Peptide Receptor 2 (Fpr2) and Toll-Like Receptor 9 (TLR9) in Schwannoma Cells-An In Vitro Model for Early Inflammatory Profiling of Schwann Cells

KORIMOVÁ, Andrea a Petr DUBOVÝ

Základní údaje

Originální název

N-Formylated Peptide Induces Increased Expression of Both Formyl Peptide Receptor 2 (Fpr2) and Toll-Like Receptor 9 (TLR9) in Schwannoma Cells-An In Vitro Model for Early Inflammatory Profiling of Schwann Cells

Autoři

KORIMOVÁ, Andrea (703 Slovensko, domácí) a Petr DUBOVÝ (203 Česká republika, garant, domácí)

Vydání

Cells, Basel, MDPI, 2020, 2073-4409

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.600

Kód RIV

RIV/00216224:14110/20:00117695

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/cells9122661

UT WoS

000601699100001

EID Scopus

2-s2.0-85098532779

Klíčová slova anglicky

Wallerian degeneration; mitochondria; disintegration; damage-associated molecular patterns; cytokines; chemokines; receptors

Štítky

14110514, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 1. 2021 10:34, Mgr. Tereza Miškechová

Anotace

V originále

Following nerve injury, disintegrated axonal mitochondria distal to the injury site release mitochondrial formylated peptides and DNA that can induce activation and inflammatory profiling of Schwann cells via formyl peptide receptor 2 (Fpr2) and toll-like receptor 9 (TLR9), respectively. We studied RT4 schwannoma cells to investigate the regulation of Fpr2 and TLR9 after stimulation with fMLF as a prototypical formylated peptide. RT4 cells were treated with fMLF at various concentrations and times with and without pretreatment with inhibitors (chloroquine for activated TLR9, PBP10 for Fpr2). Western blots of Fpr2, TLR9, p-p38, p-NF kappa B, and IL-6 were compared in relation to inflammatory profiling of RT4 cells and chemokine receptors (CCR2, CXCR4) as potential co-receptors of Fpr2. fMLF stimulation upregulated Fpr2 in RT4 cells at low concentrations (10 nM and 100 nM) but higher concentrations were required (10 mu M and 50 mu M) when the cells were pretreated with an activated TLR9 inhibitor. Moreover, the higher concentrations of fMLF could modulate TLR9 and inflammatory markers. Upregulation of Fpr2 triggered by 10 nM and 100 nM fMLF coincided with higher levels of chemokine receptors (CCR2, CXCR4) and PKC beta. Treating RT4 cells with fMLF, as an in vitro model of Schwann cells, uncovered Schwann cells' complex responses to molecular patterns of release from injured axonal mitochondria.

Návaznosti

MUNI/A/0975/2019, interní kód MU

Název: Studium změn ve strukturách nervové soustavy po poškození

Investor: Masarykova univerzita, Studium změn ve strukturách nervové soustavy po poškození, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

KORIMOVÁ, Andrea a Petr DUBOVÝ. N-Formylated Peptide Induces Increased Expression of Both Formyl Peptide Receptor 2 (Fpr2) and Toll-Like Receptor 9 (TLR9) in Schwannoma Cells-An In Vitro Model for Early Inflammatory Profiling of Schwann Cells. Cells. Basel: MDPI, 2020, roč. 9, č. 12, s. 1-14. ISSN 2073-4409. Dostupné z: https://dx.doi.org/10.3390/cells9122661.

@article{1725956,
   author = {Korimová, Andrea and Dubový, Petr},
   article_location = {Basel},
   article_number = {12},
   doi = {http://dx.doi.org/10.3390/cells9122661},
   keywords = {Wallerian degeneration; mitochondria; disintegration; damage-associated molecular patterns; cytokines; chemokines; receptors},
   language = {eng},
   issn = {2073-4409},
   journal = {Cells},
   title = {N-Formylated Peptide Induces Increased Expression of Both Formyl Peptide Receptor 2 (Fpr2) and Toll-Like Receptor 9 (TLR9) in Schwannoma Cells-An In Vitro Model for Early Inflammatory Profiling of Schwann Cells},
   url = {https://www.mdpi.com/2073-4409/9/12/2661},
   volume = {9},
   year = {2020}
}

TY  - JOUR
ID  - 1725956
AU  - Korimová, Andrea - Dubový, Petr
PY  - 2020
TI  - N-Formylated Peptide Induces Increased Expression of Both Formyl Peptide Receptor 2 (Fpr2) and Toll-Like Receptor 9 (TLR9) in Schwannoma Cells-An In Vitro Model for Early Inflammatory Profiling of Schwann Cells
JF  - Cells
VL  - 9
IS  - 12
SP  - 1-14
EP  - 1-14
PB  - MDPI
SN  - 20734409
KW  - Wallerian degeneration
KW  - mitochondria
KW  - disintegration
KW  - damage-associated molecular patterns
KW  - cytokines
KW  - chemokines
KW  - receptors
UR  - https://www.mdpi.com/2073-4409/9/12/2661
L2  - https://www.mdpi.com/2073-4409/9/12/2661
N2  - Following nerve injury, disintegrated axonal mitochondria distal to the injury site release mitochondrial formylated peptides and DNA that can induce activation and inflammatory profiling of Schwann cells via formyl peptide receptor 2 (Fpr2) and toll-like receptor 9 (TLR9), respectively. We studied RT4 schwannoma cells to investigate the regulation of Fpr2 and TLR9 after stimulation with fMLF as a prototypical formylated peptide. RT4 cells were treated with fMLF at various concentrations and times with and without pretreatment with inhibitors (chloroquine for activated TLR9, PBP10 for Fpr2). Western blots of Fpr2, TLR9, p-p38, p-NF kappa B, and IL-6 were compared in relation to inflammatory profiling of RT4 cells and chemokine receptors (CCR2, CXCR4) as potential co-receptors of Fpr2. fMLF stimulation upregulated Fpr2 in RT4 cells at low concentrations (10 nM and 100 nM) but higher concentrations were required (10 mu M and 50 mu M) when the cells were pretreated with an activated TLR9 inhibitor. Moreover, the higher concentrations of fMLF could modulate TLR9 and inflammatory markers. Upregulation of Fpr2 triggered by 10 nM and 100 nM fMLF coincided with higher levels of chemokine receptors (CCR2, CXCR4) and PKC beta. Treating RT4 cells with fMLF, as an in vitro model of Schwann cells, uncovered Schwann cells' complex responses to molecular patterns of release from injured axonal mitochondria.
ER  -

KORIMOVÁ, Andrea a Petr DUBOVÝ. N-Formylated Peptide Induces Increased Expression of Both Formyl Peptide Receptor 2 (Fpr2) and Toll-Like Receptor 9 (TLR9) in Schwannoma Cells-An In Vitro Model for Early Inflammatory Profiling of Schwann Cells. \textit{Cells}. Basel: MDPI, 2020, roč.~9, č.~12, s.~1-14. ISSN~2073-4409. Dostupné z: https://dx.doi.org/10.3390/cells9122661.

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