High fungal substrate specificity limits the utility of
environmental DNA to detect fungal diversity in bogs

J 2021

High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs

VAŠUTOVÁ, Martina; Martin JIROUŠEK a Michal HÁJEK

Základní údaje

Originální název

High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs

Autoři

VAŠUTOVÁ, Martina; Martin JIROUŠEK a Michal HÁJEK

Vydání

Ecological Indicators, Amsterdam, Elsevier, 2021, 1470-160X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.263

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00118817

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Biodiversity; eDNA; Fruitbody; Fungi; Peatland

Štítky

14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 2. 2021 10:26, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Fungi are a highly diverse group of organisms and play a significant role in decomposition and carbon cycling in boreal ecosystems. To determine how fungal communities are structured in peat bogs and how to obtain representative samples for monitoring fungal community changes, we separately sampled and sequenced (ITS2, Illumina MiSeq) peat, mixed litter and litter from individual dominant plant species in five permanent plots in raised bogs in the Jizerské Hory Mountains (Czech Republic). In total, we detected 68–103 OTUs per plot. The fungal assemblages were mostly influenced by substrate identity, whereas the effect of the site was minimal. Only a few identified OTUs behave like generalists. The most specific fungi were found in dead parts of Calluna vulgaris, Trichophorum cespitosum and Drosera rotundifolia. The high substrate heterogeneity means that sequencing soil core samples does not provide an exhaustive inventory of the fungal diversity in bogs. Poor correspondence was observed between the recorded OTUs and either visually inspected fruitbodies or literature reports on the fungal taxa associated with the analysed substrates.

Návaznosti

GX19-28491X, projekt VaV

Název: Centrum pro evropské vegetační syntézy (CEVS) (Akronym: CEVS)

Investor: Grantová agentura ČR, Centrum pro evropské vegetační syntézy (CEVS)

Citovat

VAŠUTOVÁ, Martina; Martin JIROUŠEK a Michal HÁJEK. High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs. Ecological Indicators. Amsterdam: Elsevier, 2021, roč. 121, February, s. 1-10. ISSN 1470-160X. Dostupné z: https://doi.org/10.1016/j.ecolind.2020.107009.

@article{1725979,
   author = {Vašutová, Martina and Jiroušek, Martin and Hájek, Michal},
   article_location = {Amsterdam},
   article_number = {February},
   doi = {https://doi.org/10.1016/j.ecolind.2020.107009},
   keywords = {Biodiversity; eDNA; Fruitbody; Fungi; Peatland},
   language = {eng},
   issn = {1470-160X},
   journal = {Ecological Indicators},
   title = {High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs},
   url = {https://doi.org/10.1016/j.ecolind.2020.107009},
   volume = {121},
   year = {2021}
}

TY  - JOUR
ID  - 1725979
AU  - Vašutová, Martina - Jiroušek, Martin - Hájek, Michal
PY  - 2021
TI  - High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs
JF  - Ecological Indicators
VL  - 121
IS  - February
SP  - 1-10
EP  - 1-10
PB  - Elsevier
SN  - 1470160X
KW  - Biodiversity
KW  - eDNA
KW  - Fruitbody
KW  - Fungi
KW  - Peatland
UR  - https://doi.org/10.1016/j.ecolind.2020.107009
L2  - https://doi.org/10.1016/j.ecolind.2020.107009
N2  - Fungi are a highly diverse group of organisms and play a significant role in decomposition and carbon cycling in boreal ecosystems. To determine how fungal communities are structured in peat bogs and how to obtain representative samples for monitoring fungal community changes, we separately sampled and sequenced (ITS2, Illumina MiSeq) peat, mixed litter and litter from individual dominant plant species in five permanent plots in raised bogs in the Jizerské Hory Mountains (Czech Republic). In total, we detected 68–103 OTUs per plot. The fungal assemblages were mostly influenced by substrate identity, whereas the effect of the site was minimal. Only a few identified OTUs behave like generalists. The most specific fungi were found in dead parts of Calluna vulgaris, Trichophorum cespitosum and Drosera rotundifolia. The high substrate heterogeneity means that sequencing soil core samples does not provide an exhaustive inventory of the fungal diversity in bogs. Poor correspondence was observed between the recorded OTUs and either visually inspected fruitbodies or literature reports on the fungal taxa associated with the analysed substrates.
ER  -

VAŠUTOVÁ, Martina; Martin JIROUŠEK a Michal HÁJEK. High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs. \textit{Ecological Indicators}. Amsterdam: Elsevier, 2021, roč.~121, February, s.~1-10. ISSN~1470-160X. Dostupné z: https://doi.org/10.1016/j.ecolind.2020.107009.

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